Abcg5 (NM_053754) Rat Tagged ORF Clone

CAT#: RR201020

Abcg5 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family G (WHITE), member 5 (Abcg5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcg5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201020 representing NM_053754
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGCTGCCCTTTCTGAGTCCAGAGGGAGCCAGAGGGCCTCACAACAACAGAGGGTCTCAGAGCT
  CCCTGGAGGAAGGCTCAGTTACAGGCTCAGAGGCTCGGCACAGCTTAGGTGTCCTGAATGTGTCCTTCAG
  CGTCAGCAACCGTGTCGGGCCCTGGTGGAACATCAAATCATGCCAGCAGAAGTGGGACAGGAAAATCCTC
  AAAGATGTCTCCTTGTACATCGAGAGTGGCCAGACCATGTGCATCTTAGGTAGCTCAGGCTCAGGGAAAA
  CCACGCTGCTGGACGCCATCTCTGGGAGGCTGCGGCGCACAGGGACCTTGGAAGGGGAAGTGTTTGTGAA
  CGGCTGCGAGCTGCGCAGGGACCAGTTCCAAGACTGCGTCTCCTACCTCCTGCAGAGCGATGTCTTTCTG
  AGCAGCCTCACGGTGCGGGAGACGCTGAGATACACGGCGATGCTGGCTCTCCGCAGCAGCTCCGCGGACT
  TCTACGACAAGAAGGTAGAGGCAGTCCTGACAGAGCTGAGTCTGAGCCACGTGGCAGACCAAATGATCGG
  CAACTATAATTTTGGGGGGATTTCCAGTGGCGAGCGGCGCCGAGTGTCCATCGCAGCCCAACTCCTTCAG
  GACCCCAAGGTCATGATGCTTGACGAGCCAACCACAGGACTGGACTGCATGACTGCAAATCATATCGTCC
  TCCTCTTGGTCGAGCTGGCTCGCAGGAACCGCATTGTAATTGTCACCATCCACCAGCCTCGCTCTGAGCT
  CTTCCACCACTTCGACAAAATTGCCATTCTGACTTACGGAGAGTTGGTGTTCTGTGGCACGCCAGAGGAG
  ATGCTCGGCTTCTTCAATAACTGTGGTTACCCCTGTCCTGAACATTCCAATCCCTTTGATTTCTACATGG
  ACTTGACATCGGTGGACACCCAAAGCAGAGAGCGAGAGATAGAGACGTACAAGCGAGTCCAGATGCTGGA
  ATCTGCCTTCAGGCAATCGGACATCTGTCACAAAATCCTGGAGAACATTGAAAGAACAAGACACCTGAAA
  ACCCTACCCATGGTTCCTTTCAAAACGAAAAATCCTCCCGGAATGTTCTGCAAGCTCGGCGTTCTCCTGA
  GGAGAGTAACGAGAAACCTAATGAGGAATAAGCAGGTGGTGATTATGCGTCTTGTTCAGAATCTGATCAT
  GGGTCTGTTCCTCATTTTCTACCTTCTCCGAGTCCAGAACAACATGCTGAAGGGCGCTGTTCAGGACCGC
  GTAGGGCTGTTGTACCAGCTTGTGGGTGCCACCCCGTACACCGGCATGCTCAACGCTGTGAACCTCTTTC
  CCATGCTGAGAGCTGTCAGCGACCAGGAGAGTCAGGATGGCCTGTACCAGAAGTGGCAGATGCTGCTCGC
  CTATGTGCTGCATGCTCTCCCCTTCAGCATCGTTGCCACGGTGATTTTCAGCAGCGTGTGTTACTGGACT
  CTGGGCTTGTATCCCGAGGTCGCCAGATTTGGATACTTCTCTGCCGCTCTGTTGGCCCCTCACTTAATTG
  GAGAATTTCTGACACTTGTGCTGCTTGGTATGGTCCAAAACCCCAATATTGTCAACAGCATAGTGGCTCT
  GCTGAGTATTTCTGGGTTGCTCATTGGATCTGGATTTATCAGAAACATAGAAGAAATGCCCATTCCTTTA
  AAAATCCTGGGTTACTTTACCTTCCAAAAGTACTGTTGTGAGATTCTTGTGGTCAATGAGTTCTATGGCC
  TGAACTTCACTTGTGGTGGCTCCAACACTTCTGTGCCAAATAACCCAATGTGTTCCATGACCCAAGGGAT
  CCAATTCATTGAGAAAACCTGCCCAGGGGCCACGTCCAGATTCACGACAAACTTCCTGATCTTGTACTCG
  TTCATCCCGACTCTTGTCATCCTGGGGATGGTGGTCTTTAAAGTCCGGGACTACCTGATTAGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201020 representing NM_053754
  Red=Cloning site Green=Tags(s)
  
  MSELPFLSPEGARGPHNNRGSQSSLEEGSVTGSEARHSLGVLNVSFSVSNRVGPWWNIKSCQQKWDRKIL
  KDVSLYIESGQTMCILGSSGSGKTTLLDAISGRLRRTGTLEGEVFVNGCELRRDQFQDCVSYLLQSDVFL
  SSLTVRETLRYTAMLALRSSSADFYDKKVEAVLTELSLSHVADQMIGNYNFGGISSGERRRVSIAAQLLQ
  DPKVMMLDEPTTGLDCMTANHIVLLLVELARRNRIVIVTIHQPRSELFHHFDKIAILTYGELVFCGTPEE
  MLGFFNNCGYPCPEHSNPFDFYMDLTSVDTQSREREIETYKRVQMLESAFRQSDICHKILENIERTRHLK
  TLPMVPFKTKNPPGMFCKLGVLLRRVTRNLMRNKQVVIMRLVQNLIMGLFLIFYLLRVQNNMLKGAVQDR
  VGLLYQLVGATPYTGMLNAVNLFPMLRAVSDQESQDGLYQKWQMLLAYVLHALPFSIVATVIFSSVCYWT
  LGLYPEVARFGYFSAALLAPHLIGEFLTLVLLGMVQNPNIVNSIVALLSISGLLIGSGFIRNIEEMPIPL
  KILGYFTFQKYCCEILVVNEFYGLNFTCGGSNTSVPNNPMCSMTQGIQFIEKTCPGATSRFTTNFLILYS
  FIPTLVILGMVVFKVRDYLISR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053754
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053754.1, NM_053754.2, NP_446206.2
• RefSeq Size: 2470
• RefSeq ORF: 1959
• Locus ID: 114628
• MW: 73.4 kDa
• Gene Summary: ATP-transporter family member that may regulate absorption of dietary cholesterol; human homolog is mutated in sitosterolemia, a disease of hyperabsorption of cholesterol and other sterols [RGD, Feb 2006]