Akt1 (NM_033230) Rat Tagged ORF Clone

CAT#: RR201042

  • TrueORF®

Akt1 (Myc-DDK-tagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 1 (Akt1), (10 ug)


  "NM_033230" in other vectors (3)

Reconstitution Protocol

USD 650.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Akt1
Synonyms Akt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR201042 representing NM_033230
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGACGTAGCCATTGTGAAGGAGGGCTGGCTGCACAAACGAGGGGAATATATTAAAACCTGGCGGC
CACGCTACTTCCTCCTCAAGAATGATGGCACCTTTATTGGCTACAAGGAACGGCCTCAGGATGTGGAGCA
GCGCGAGTCCCCACTCAACAACTTCTCAGTGGCACAATGTCAGCTGATGAAGACAGAGCGGCCGAGGCCC
AACACCTTCATCATCCGCTGCCTGCAGTGGACCACGGTCATTGAGCGCACCTTCCATGTGGAAACGCCTG
AGGAGCGGGAAGAGTGGACCACCGCCATTCAGACTGTGGCTGATGGACTCAAACGGCAGGAGGAGGAGAC
GATGGACTTCCGGTCAGGTTCACCCAGTGACAACTCAGGTGCTGAGGAGATGGAGGTGGCCCTGGCCAAG
CCCAAGCACCGTGTGACCATGAACGAGTTTGAGTACCTGAAGCTACTGGGCAAGGGCACCTTTGGGAAGG
TGATCCTGGTGAAGGAGAAGGCCACAGGTCGCTACTATGCCATGAAGATCCTCAAGAAGGAGGTCATCGT
TGCCAAGGATGAGGTTGCCCACACGCTTACTGAGAACCGTGTCCTGCAGAACTCTAGGCATCCCTTCCTT
ACAGCCCTCAAGTACTCATTCCAGACCCACGACCGCCTCTGCTTTGTCATGGAGTACGCCAATGGAGGCG
AGCTCTTCTTCCACCTGTCTCGTGAGCGCGTGTTTTCAGAGGACCGGGCCCGCTTCTACGGTGCGGAGAT
TGTGTCCGCCCTGGACTACTTGCACTCCGAGAAGAACGTGGTGTACCGGGACCTCAAGCTGGAGAACCTC
ATGCTGGACAAGGACGGGCACATCAAGATAACGGACTTCGGGCTGTGCAAGGAGGGTATCAAGGACGGTG
CCACCATGAAGACGTTCTGCGGGACACCCGAGTACCTGGCCCCTGAGGTGCTGGAGGACAACGACTATGG
CCGTGCAGTGGACTGGTGGGGGCTGGGCGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCCTTCTAC
AACCAGGACCATGAGAAGCTGTTCGAGCTCATCCTAATGGAGGAGATCCGCTTCCCACGCACACTCGGGC
CGGAGGCCAAGTCCCTGCTCTCGGGGCTGCTCAAGAAGGACCCTACACAGAGGCTCGGTGGGGGCTCCGA
GGACGCCAAGGAGATCATGCAGCACCGCTTCTTTGCCAACATCGTGTGGCAAGATGTGTATGAGAAGAAG
CTGAGCCCACCTTTCAAGCCCCAGGTCACCTCTGAGACCGACACCAGGTATTTTGATGAGGAGTTCACAG
CTCAGATGATCACCATCACACCGCCTGATCAAGATGACAGCATGGAGTGTGTGGACAGTGAACGGAGGCC
GCACTTTCCCCAGTTCTCCTACTCAGCCAGTGGCACAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR201042 representing NM_033230
Red=Cloning site Green=Tags(s)

MNDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVEQRESPLNNFSVAQCQLMKTERPRP
NTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKRQEEETMDFRSGSPSDNSGAEEMEVALAK
PKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFL
TALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENL
MLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFY
NQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPTQRLGGGSEDAKEIMQHRFFANIVWQDVYEKK
LSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033230
ORF Size 1440 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_033230.1, NM_033230.2, NP_150233.1
RefSeq Size 1613
RefSeq ORF 1443
Locus ID 24185
MW 55.7 kDa
Gene Summary inhibits JUN kinase activation and mediates inhibition of apoptosis [RGD, Feb 2006]

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