Dusp6 (NM_053883) Rat Tagged ORF Clone

CAT#: RR201091

Dusp6 (Myc-DDK-tagged ORF) - Rat dual specificity phosphatase 6 (Dusp6), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dusp6
• Synonyms: Mkp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201091 representing NM_053883
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGATACGCTCAGACCCGTGCCCTTCGCGTCGGAAATGGCGATCAGCAAGACGGTGGCGTGGCTCA
  ACGAGCAGCTGGAGCTGGGCAACGAACAGCTGCTGCTGATGGACTGCCGACCGCAGGAGCTGTACGAGTC
  GTCGCACATCGAATCTGCCATCAACGTGGCCATCCCGGGCATCATGCTGCGGCGTCTGCAGAAGGGCAAC
  CTGCCGGTGCGCGCGCTATTCACGCGCTGCGAGGACCGGGACCGCTTTACCAGGCGCTGCGGCACCGACA
  CCGTGGTGCTCTACGACGAGAACAGCAGCGACTGGAATGAGAACACAGGTGGAGAGTCGGTCCTCGGGCT
  GCTGCTCAAGAAACTCAAAGACGAGGGCTGCCGGGCGTTCTACCTTGAAGGTGGCTTCAGTAAGTTCCAG
  GCCGAGTTCGCCCTGCACTGCGAGACCAATCTAGACGGCTCGTGCAGCAGCAGCTCCCCGCCCTTGCCAG
  TGCTGGGACTCGGGGGCCTGAGGATCAGCTCCGACTCTTCCTCGGACATTGAGTCTGACCTTGACCGAGA
  CCCCAATAGTGCAACGGACTCCGATGGCAGCCCGCTGTCCAACAGCCAGCCTTCCTTCCCGGTGGAGATT
  TTGCCCTTCCTTTACCTGGGCTGTGCCAAGGACTCTACTAACTTGGACGTGTTGGAAGAGTTTGGCATCA
  AGTACATCTTGAACGTCACCCCCAATCTGCCCAATCTGTTTGAGAATGCAGGGGAGTTCAAGTACAAGCA
  AATTCCTATCTCTGATCACTGGAGCCAAAACCTGTCCCAGTTTTTCCCTGAGGCCATTTCTTTCATAGAT
  GAAGCCCGAGGCAAAAACTGTGGTGTCCTGGTGCATTGCTTGGCGGGCATCAGCCGCTCCGTCACGGTGA
  CAGTGGCTTACCTTATGCAGAAGCTCAACTTGTCCATGAACGATGCTTATGACATTGTCAAAATGAAGAA
  GTCCAACATCTCTCCCAACTTCAACTTCATGGGCCAGCTGCTTGACTTTGAAAGGACCCTGGGACTCAGC
  AGCCCCTGTGACAATCGTGTCCCCGCACAGCAGCTCTACTTCACCGCGCCCTCCAACCAGAATGTCTACC
  AAGTGGACTCCCTGCAATCTACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201091 representing NM_053883
  Red=Cloning site Green=Tags(s)
  
  MIDTLRPVPFASEMAISKTVAWLNEQLELGNEQLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGN
  LPVRALFTRCEDRDRFTRRCGTDTVVLYDENSSDWNENTGGESVLGLLLKKLKDEGCRAFYLEGGFSKFQ
  AEFALHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEI
  LPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFID
  EARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLS
  SPCDNRVPAQQLYFTAPSNQNVYQVDSLQST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053883
• ORF Size: 1143 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053883.1, NM_053883.2, NP_446335.1
• RefSeq Size: 2781
• RefSeq ORF: 1146
• Locus ID: 116663
• MW: 42.3 kDa
• Gene Summary: protein tyrosine phosphatase involved in regulation of mitogen-activated protein kinase (MAPK) [RGD, Feb 2006]