Bag5 (NM_001008526) Rat Tagged ORF Clone

CAT#: RR201099

Bag5 (Myc-DDK-tagged ORF) - Rat BCL2-associated athanogene 5 (Bag5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bag5
• Synonyms: MGC95040
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201099 representing NM_001008526
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATGGGAAACCAACACCCTTCTATTAGTAGGCTTCAGGAGATCCAACGGGAAGTAAAGAGTATAG
  AGCAGCAAGTGGTTGGCTTCAGTGGCCTGTCGGATGACAAGAATTACAAGAGACTGGAGAGGATTCTGAC
  GAAACAACTTTTTGAAATCGACTCTGTGGATACAGAAGGGAAAGGAGACATCCAGCAAGCTAGGAAGAGG
  GCAGCACAGGACACAGAACGCCTTCTCAAAGAGTTGGAGCAGAACGCGAACCACCCACACCGGATTGAAA
  TCAAGAATATCTTTCAAGAAGCTCAGGCCCTTGTGAAAGAAAAGACTGTGCCCTTTTACAGTGGAAGCAA
  CTGTGTGACTAGCGAGTTTGAGGAAGCCATCCAGGACATCATTCTGAGGCTGACACATGTTAAAACAGGA
  GGGAAGATCTCCTTGCGGAAAGCAAGGTACCACACACTAACCAAGATCTGTGCGGTACAAGAGATTATTG
  AAGACTGTGTGAGGAAACAGCCTTCCCTGCCACTTTCCGAGGATGTGCATCCTTCTGTCGCCAAGATCAA
  TTCTGTGATGTGTGAGGTGAACAAGGCCAGAGGTACTCTGATTGCACTTCTGATGGGCGTGGACAGTTCT
  GAGACTTGCAGGCACTTATCATGCGTGCTGTCAGGACTGATGGCTGATTTAGATGCTCTAGATGTGTGTG
  GGCGTACAGAGATCAGAAATTATCGGAGGGAGGTGGTGGAAGATATCAACAAATTACTAAAATATCTAGA
  TTTAGAAGAGGAAGCTGACAATACACATGCATTTGACCTGGGACAGAACCATTCCATTATAAAGATAGAA
  AATGTCCTCAAGAGGATGAGAGAAATGAAAAATGAACTTCTCCAGGCACAGAGCCCTCCTGAATTGTACC
  TGAGGTCCAAGACAGAGCTGCAGGGCTTGATCGGGCAGCTGGACGAAGTGAGTCTGGAGAAAAACCCCTG
  CATCAGAGAGGCCAGGAGAAGAGCAGTGATTGAGGTGCAGACCCTCATCACGTACCTGGACCTGAAGGAG
  GCCCTCGAGAAGAGGAAGCTGTTCCCGTGTGAGGAGACCCCCCCACATAAGGCTGTGTGGAATGTCCTTG
  GAAACCTGTCAGAGATCCAGGGTGAAGTCCTCTCCTTTGGTGGGAACCGAACTGATAAGAACTACATTCG
  TCTGGAGGAGCTACTGACCAAACAGTTGCTGACTCTGGATGCTGTTGACCCACTGGGAGAAGAGAAGTGT
  AAGGCTGCCAGGAAGCAGGCCGTGAAGCTGGCCCAGAACATCCTCAGCTACCTTGACATGAAGTCTGATG
  AGTGGGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201099 representing NM_001008526
  Red=Cloning site Green=Tags(s)
  
  MDMGNQHPSISRLQEIQREVKSIEQQVVGFSGLSDDKNYKRLERILTKQLFEIDSVDTEGKGDIQQARKR
  AAQDTERLLKELEQNANHPHRIEIKNIFQEAQALVKEKTVPFYSGSNCVTSEFEEAIQDIILRLTHVKTG
  GKISLRKARYHTLTKICAVQEIIEDCVRKQPSLPLSEDVHPSVAKINSVMCEVNKARGTLIALLMGVDSS
  ETCRHLSCVLSGLMADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADNTHAFDLGQNHSIIKIE
  NVLKRMREMKNELLQAQSPPELYLRSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYLDLKE
  ALEKRKLFPCEETPPHKAVWNVLGNLSEIQGEVLSFGGNRTDKNYIRLEELLTKQLLTLDAVDPLGEEKC
  KAARKQAVKLAQNILSYLDMKSDEWEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008526
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008526.1, NM_001008526.2, NP_001008526.1
• RefSeq Size: 2040 bp
• RefSeq ORF: 1344 bp
• Locus ID: 366734
• Cytogenetics: 6q32
• MW: 51 kDa