Cd96 (NM_001025032) Rat Tagged ORF Clone

CAT#: RR201209

Cd96 (Myc-DDK-tagged ORF) - Rat CD96 molecule (Cd96), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd96
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201209 representing NM_001025032
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGAAAATGGACATACTGTGTGGTTTACGCCATCATCCAGATACAGTTTTTCAGGGGAGCATGGG
  AAGAACTATTCAATGCTGGAGATAACAACGTGTATGCTCTGCCTGGTTCTGATGTCAACTTGACCTGCCA
  AACAATGGAGAAAGACTTAATGGTTCAGATGCAGTGGTCCAAGGTCACAGATGAGATTGATATGATTGTT
  GTTTATCATCCCCAATATGGCTTCCACTATATGCAGGGGGTTGCCTGTGAGTCACGAGTGGCTGCGGTAG
  AAACTCTGAAGGATGCAACGAAATGGACTCTGAACTTAAGGAATATCTCTTCCAGCCTCAGTGGAAAGTA
  TGAGTGCAGCTTCACTATGTATCCAACAGGCACCAAGACTATAGTCTACAACCTCATTGTGGAACCCTAT
  ACACAAGATGAACACAACCGTACAATAGAAATAGAGACAAATCGGACTCTGGAAATACCATGCTTTCAAA
  ATACCTCCTCAGAAATTTCACCTAGGTTCACCTTTTCATGGTTGGTGGAGAAAGATGGAGTGGAAGACGT
  TCTCTTCACCTACGATTACCACGTCAGCAATTCCACAGCATTTAAAGGCAGAGTTGGGCTGGGTGCAGAC
  TATGGACTCCGCCTCTCCCCAGTCCAAATCCAAGATGATGGCAGGACATTCTCTTGCTTTCTGAGAATCA
  GTCCTCTCAAAGTCTGGAAGACATCCACCACAGTCAAGGTTTTTGCTAAGCCAGAAATCCTCATGACTGT
  GGAAAACACCACCATGGATGTCTTAGGAGAGAGAGTATTTACCTGCCTTCTGAAGAATGTTTTCCCCAAA
  GCAAGTATTACCTGGTTAATAGATGGAAGACTTTTTCAAGGCAATGAAGAAGGAATATACATTACAAATG
  AAGAAAAAAATAGCAGTAGCGGATTTTGGGAACTGAAGTCGGTTCTAACAAGGATGCACAATAGAACATC
  CCAATCAAACAATATGACAGTTTGGTGTATGGCTCTGTCGCCAGGCCCTGGAAATAAAATGTGGAATACT
  TCATCACAACCCATCACATTTTCCTTGGATTCTGGGACTGCCCCAACAAAACGTCTACCCAATGTGACAG
  GTTCTACCCTGGGTGCACAAACCTTTCCAGATGCCGAAGTATCTCCTACAAGATATCTAGCTACATCTTC
  AATGACAATTGTAGATGAAAATGTGTTGACACCAGACCCAACTCCTCAAACCAGCAATTCCAGCATGACT
  ACTAAAGATGTCAACTATTCCCAGCCCTCCAGTGGGACAGATGCCAAGAACTCCTCAAGAGCTGCTTCTT
  CTTCTGATGGTGGATCTCGGCCTTTTCCTTCCACCTCTCCTCCCAAATGGCTCTCACTGCCTCATACTTC
  CACTGGACCCCAGGAACCAGACTCCGCAGTTTCATGGATACCAACAGATGCGTACACTTCAGGCTCTTCA
  GATGCAAGCTTAACTTCTCACGATGTCATCATCCGTACAACCAAAGAATTTCCAGATGTCCTCACAACTG
  CAAATGGAACTACTAAAATTAAACATGGCCACGTCACAGGAATCACAGTTAATAAGCCCAGAGATGGAAT
  GTCCTGGCCTGTGGCTGTCGCGACTTTGCTATTTTTCTGCATACTGTTGTTTGGGCTTGGAGTGAGAAAA
  TGGTGTCAGTATCAAAAGGAAATCATGGAAAGACCCCCACCTTTCAAGCCACCACCACCTCCCATCAAGT
  ACATGTGCATTCAGGAACCCACTGGACGTGGCATGCCTTGTCATGAGATGGAGGTCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201209 representing NM_001025032
  Red=Cloning site Green=Tags(s)
  
  MGRKWTYCVVYAIIQIQFFRGAWEELFNAGDNNVYALPGSDVNLTCQTMEKDLMVQMQWSKVTDEIDMIV
  VYHPQYGFHYMQGVACESRVAAVETLKDATKWTLNLRNISSSLSGKYECSFTMYPTGTKTIVYNLIVEPY
  TQDEHNRTIEIETNRTLEIPCFQNTSSEISPRFTFSWLVEKDGVEDVLFTYDYHVSNSTAFKGRVGLGAD
  YGLRLSPVQIQDDGRTFSCFLRISPLKVWKTSTTVKVFAKPEILMTVENTTMDVLGERVFTCLLKNVFPK
  ASITWLIDGRLFQGNEEGIYITNEEKNSSSGFWELKSVLTRMHNRTSQSNNMTVWCMALSPGPGNKMWNT
  SSQPITFSLDSGTAPTKRLPNVTGSTLGAQTFPDAEVSPTRYLATSSMTIVDENVLTPDPTPQTSNSSMT
  TKDVNYSQPSSGTDAKNSSRAASSSDGGSRPFPSTSPPKWLSLPHTSTGPQEPDSAVSWIPTDAYTSGSS
  DASLTSHDVIIRTTKEFPDVLTTANGTTKIKHGHVTGITVNKPRDGMSWPVAVATLLFFCILLFGLGVRK
  WCQYQKEIMERPPPFKPPPPPIKYMCIQEPTGRGMPCHEMEVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025032
• ORF Size: 1809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025032.1, NP_001020203.1
• RefSeq Size: 2079 bp
• RefSeq ORF: 1812 bp
• Locus ID: 498079
• Cytogenetics: 11q21
• MW: 67.2 kDa