Wif1 (NM_053738) Rat Tagged ORF Clone

CAT#: RR201224

Wif1 (Myc-DDK-tagged ORF) - Rat Wnt inhibitory factor 1 (Wif1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wif1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201224 representing NM_053738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGAGAAGAGCCTTCCCTGCTTTCGTGCTCCGGCTCTGGAGCATCCTACCTTGCCTGCTCCTGC
  TACGAGCGGATGCAGGGCAGCCGCCAGAGGAGAGCTTGTACCTGTGGATCGACGCCCATCAGGCCAGAGT
  ACTCATAGGATTTGAAGAAGATATTCTGATTGTCTCGGAGGGGAAAATGGCCCCCTTTACACATGATTTC
  AGGAAAGCCCAACAAAGAATGCCAGCCATTCCCGTCAATATCCACTCCATGAATTTTACCTGGCAAGCTT
  CAGGGCAGGCAGAGTACTTCTATGAGTTCCTGTCGCTGCGCTCGCTGGATAAAGGCATCATGGCAGACCC
  AACTGTCAATGTCCCTCGGCTGGGAACAGTGCCTCACAAGGCATCAGTTGTTCAAGTTGGTTTCCCGTGT
  CTCGGCAAACAGGATGGGGTGGCAGCATTTGAAGTGAATGTGATTGTCATGAATTCTGAAGGCAACCCCA
  TCCTTCGGACCCCTCAAAATGCTATCTTCTTTAAAACATGTCAACAAGCTGAGTGCCCAGGAGGGTGTCG
  AAATGGAGGCTTTTGTAACGAAAGGCGGGTCTGCGAGTGTCCCGATGGGTTCTATGGACCTCACTGTGAG
  AAAGCCCTCTGCATACCTCGATGTATGAACGGTGGTCTGTGTGTCACTCCTGGCTTCTGCATCTGCCCGC
  CTGGATTCTACGGTGTCAACTGTGACAAAGCAAACTGCTCGGCCACCTGCTTTAATGGAGGGACCTGTTT
  TTACCCAGGAAAATGTATTTGCCCTCCAGGACTTGAGGGAGAGCAGTGTGAACTCAGCAAGTGCCCCCAA
  CCCTGCCGAAACGGAGGTAAATGCATTGGTAAAAGCAAGTCTGTCTGCGAGCCTGGCTGCGGTGCCCATG
  GAACCTGCCACGAACCCAACAAATGCCAGTGTCGAGAGGGCTGGCATGGGAGACACTGCAATAAAAGGTA
  CGGAGCCAGCCTCATGCATGCCCCGAGGCCAGCAGGCGCCGGGCTGGAGCGGCACACGCCTTCACTTAAA
  AAGGCTGAGGGGCGGAGGGATCCACCTGAATCCAATTACATCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201224 representing NM_053738
  Red=Cloning site Green=Tags(s)
  
  MARRRAFPAFVLRLWSILPCLLLLRADAGQPPEESLYLWIDAHQARVLIGFEEDILIVSEGKMAPFTHDF
  RKAQQRMPAIPVNIHSMNFTWQASGQAEYFYEFLSLRSLDKGIMADPTVNVPRLGTVPHKASVVQVGFPC
  LGKQDGVAAFEVNVIVMNSEGNPILRTPQNAIFFKTCQQAECPGGCRNGGFCNERRVCECPDGFYGPHCE
  KALCIPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSATCFNGGTCFYPGKCICPPGLEGEQCELSKCPQ
  PCRNGGKCIGKSKSVCEPGCGAHGTCHEPNKCQCREGWHGRHCNKRYGASLMHAPRPAGAGLERHTPSLK
  KAEGRRDPPESNYIW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053738
• ORF Size: 1095 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053738.1, NP_446190.1
• RefSeq Size: 2182
• RefSeq ORF: 1098
• Locus ID: 114557
• MW: 40.5 kDa
• Gene Summary: human homolog has decreased mRNA expression in some prostate cancers and other cancers; may act as a secreted inhibitor of the Wnt signaling pathway [RGD, Feb 2006]