Bcar1 (NM_012931) Rat Tagged ORF Clone

CAT#: RR201266

Bcar1 (Myc-DDK-tagged ORF) - Rat breast cancer anti-estrogen resistance 1 (Bcar1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcar1
• Synonyms: Cas; Crkas; P130CAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201266 representing NM_012931
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTACCTGGTGAGTGTGGGCGCGGGGCCGGCCAGGCGGGCGGGCGGTCTGGAGGACGTTTCCTGGG
  GGCCCCGAGTGTCCCGCCGGCCGCAGAGTTACCGGGCAGCTCGGCACGTAAACGAGTCCCTCCCTCGGTC
  CGCCTTTCGAGTCCCCGCAGCTCACGGAGCTAGCGTGACGCCCTCCGCAGCCCTTGGGTCCGGGTTGCCT
  GAGACTCAGCCCGAGGCGGTATGCAGAGGGACTGAGAAACCGAGATTTGCTGAGGGCTGCAAACCGGCGG
  CGAGCCGCGACAAGAACGTGCTGGCCAAAGCCCTCTATGACAACGTAGCTGAGTCTCCGGATGAACTCTC
  CTTCCGAAAGGGTGACATCATGACAGTGCTCGAGCGGGACACTCAGGGCCTGGATGGCTGGTGGCTCTGC
  TCACTGCATGGGCGCCAGGGCATTGTGCCTGGTAACCGCCTCAAGATTCTGGTTGGCATGTATGACAAGA
  AGCCAGCAGCACCTGGACCAGGCCCACCTGCCACCCCACCCCAGCCCCAGCCCAGCCTTCCCCAGGGCGT
  CCATACTCCAGTGCCTCCAGCTTCCCAGTACAGTCCCATGCTCCCCACTGCATACCAACCCCAACCTGAC
  AATGTGTACCTGGTACCCACTCCCAGCAAGACTCAGCAAGGTCTCTACCAAGCCCCTGGGCCCAACCCAC
  AGTTCCAGTCACCCCCAGCCAAACAAACATCCACATTCTCAAAGCAAACACCTCATCACTCATTTCCCAG
  CCCTGCCACAGACCTTTACCAGGTGCCTCCAGGGCCTGGAAGTCCAGCCCAGGACATTTACCAGGTGCCA
  CCTTCCGCTGGCACAGGGCATGACATCTACCAAGTCCCTCCATCTTTGGACACCCGCAGCTGGGAGGGAA
  CGAAGCCACCGGCAAAGGTGGTGGTACCTACTCGAGTGGGACAGGGCTATGTGTACGAGGCCTCCCAGGC
  AGAACAGGATGAGTACGACACCCCGCGCCACCTGCTAGCTCCAGGTTCCCAGGACATCTATGATGTGCCC
  CCTGTTCGAGGACTGCTTCCCAACCAGTATGGCCAGGAGGTATATGACACCCCGCCTATGGCAGTCAAAG
  GCCCCAATGGCCGAGACCCGTTGTTGGATGTGTATGACGTGCCCCCTAGTGTGGAGAAAGGCCTGCCGCC
  GTCCAACCATCATTCGGTGTATGATGTTCCTCCTTCTGTGAGCAAGGATGTGCCTGATGGCCCACTGCTG
  CGTGAGGAAACATATGATGTACCCCCTGCCTTCGCCAAACCCAAGCCTTTTGATCCGACCCGACATCCAT
  TAATCCTCGCTGCACCTCCTCCGGACTCCCCACCAGCTGAGGATGTGTATGATGTGCCACCCCCTGCTCC
  TGACCTCTATGATGTGCCCCCTGGTTTGCGGCGGCCTGGCCCTGGAACACTGTATGACGTGCCTCGAGAG
  CGGGTGCTCCCTCCTGAGGTGGCTGATGGGAGTGTAATTGATGATGGTGTGTATGCTGTGCCCCCACCAG
  CCGAGCGAGAGGCGCCAACAGATGGCAAGCGCCTGTCGGCCTCTAGCACGGGCAGCACACGCAGCAGCCA
  ATCGGCGTCTTCCTTGGAGGTAGTGGTGCCAGGTCGGGAGCCCCTGGAGCTGGAAGTTGCTGTGGAGACC
  CTGGCTCGGCTACAGCAGGGTGTGAGCACCACCGTAGCCCACCTTCTGGACCTGGTGGGCAGTGCCAGTG
  GCCCCGGGGGCTGGCGTAGTACCTCTGAGCCCCAGGAGCCTCCCGTGCAGGACCTGAAAGCTGCAGTGGC
  CGCAGTTCATGGGGCAGTACATGAGCTCCTGGAGTTTGCCCGCAGTGCAGTGAGCAGTGCCACTCACACT
  TCTGACCGCACTTTGCACGCCAAGCTTAGCCGGCAGCTACAGAAGATGGAGGATGTGTACCAGACACTGG
  TGGTCCATGGTCAGGTCCTTGACAGTGGCCGGGGAGGTCCAGGATTCACTCTGGACGACCTGGACCGCCT
  GGTGGCCTGCTCACGGGCTGTGCCCGAGGATGCCAAGCAGCTGGCCTCCTTTTTGCATGGTAATGCCTCG
  CTGCTTTTCAGACGGACCAAAGCCCCTGGCCCCGGGCCTGAGGGCAGCAGCTCCCTGCACCTCAACCCCA
  CCGATAAAGCCAGCAGCATCCAGTCACGCCCTCTACCCTCACCTCCAAAGTTCACCTCCCAGGACTCTCC
  GGATGGCCAGTATGAGAACAGTGAAGGGGGTTGGATGGAGGACTATGACTACGTTCATCTGCAGGGGAAG
  GAGGAATTTGAGAAGACCCAGAAGGAACTGCTGGAAAAGGGCAACATCGTGCGACAGGGAAAAGGCCAAC
  TGGAGCTGCAGCAGCTGAAACAGTTTGAGCGACTGGAGCAGGAGGTGTCTCGTCCCATAGACCATGACCT
  GGCCAACTGGACACCAGCCCAGCCCCTTGTGCCGGGGCGGACAGGGGGACTGGGGCCTTCAGACCGACAG
  CTGCTGCTCTTCTACTTGGAGCAGTGTGAGGCCAACCTGACCACACTGACAGATGCGGTGGACGCCTTCT
  TCACCGCAGTGGCCACCAACCAGCCACCCAAGATCTTTGTGGCACACAGCAAGTTTGTCATTCTTAGTGC
  ACACAAGCTTGTGTTCATTGGGGACACACTGTCACGGCAGGCAAAGGCAGCTGATGTGCGAAGCAAAGTG
  ACCCACTACAGCAATCTGCTGTGTGACCTCCTGCGTGGCATCGTGGCCACCACCAAGGCCGCTGCCCTGC
  AGTACCCATCCCCATCTGCTGCCCAGGACATGGTAGACAGGGTCAAGGAGCTAGGCCACAGCACTCAGCA
  GTTCCGCCGTGTCCTGGGCCAGCTGGCTGCCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201266 representing NM_012931
  Red=Cloning site Green=Tags(s)
  
  MKYLVSVGAGPARRAGGLEDVSWGPRVSRRPQSYRAARHVNESLPRSAFRVPAAHGASVTPSAALGSGLP
  ETQPEAVCRGTEKPRFAEGCKPAASRDKNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLC
  SLHGRQGIVPGNRLKILVGMYDKKPAAPGPGPPATPPQPQPSLPQGVHTPVPPASQYSPMLPTAYQPQPD
  NVYLVPTPSKTQQGLYQAPGPNPQFQSPPAKQTSTFSKQTPHHSFPSPATDLYQVPPGPGSPAQDIYQVP
  PSAGTGHDIYQVPPSLDTRSWEGTKPPAKVVVPTRVGQGYVYEASQAEQDEYDTPRHLLAPGSQDIYDVP
  PVRGLLPNQYGQEVYDTPPMAVKGPNGRDPLLDVYDVPPSVEKGLPPSNHHSVYDVPPSVSKDVPDGPLL
  REETYDVPPAFAKPKPFDPTRHPLILAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRE
  RVLPPEVADGSVIDDGVYAVPPPAEREAPTDGKRLSASSTGSTRSSQSASSLEVVVPGREPLELEVAVET
  LARLQQGVSTTVAHLLDLVGSASGPGGWRSTSEPQEPPVQDLKAAVAAVHGAVHELLEFARSAVSSATHT
  SDRTLHAKLSRQLQKMEDVYQTLVVHGQVLDSGRGGPGFTLDDLDRLVACSRAVPEDAKQLASFLHGNAS
  LLFRRTKAPGPGPEGSSSLHLNPTDKASSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGK
  EEFEKTQKELLEKGNIVRQGKGQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLVPGRTGGLGPSDRQ
  LLLFYLEQCEANLTTLTDAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSKV
  THYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVDRVKELGHSTQQFRRVLGQLAAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012931
• ORF Size: 2904 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012931.1, NP_037063.1
• RefSeq Size: 3335
• RefSeq ORF: 2907
• Locus ID: 25414
• MW: 104.3 kDa
• Gene Summary: SH3 domain-containing protein that may assemble signals from SH2 domain-containing proteins; important for oncogenesis, actin cytoskeleton organization and cellular migration; tyrosine phosphorylated under multiple conditions [RGD, Feb 2006]