Rpap3 (NM_001004243) Rat Tagged ORF Clone

CAT#: RR201292

Rpap3 (Myc-DDK-tagged ORF) - Rat RNA polymerase II associated protein 3 (Rpap3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rpap3
• Synonyms: MGC94954
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201292 representing NM_001004243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCGACAAGTAAGGCGGTTGAACTGCAGCTCCAGGTGAAGCACAATGCGGAGGAACTGCAGGATT
  TCATGAGGGACTTGGAGCACTGGGAAAAGACTATGAGGCAGAAGGATCTGGAGCTCAGAAGGCAGAGTGG
  CGTTCCCGAGGAGAATTTACCTCCTATTCGAAATGGGAGTTTTAGGAAAAAGAAGAAACGTAAAACTAAA
  GATTCTTCCAAAAAAACCAAAGAAGAAAACACAAAAAACAGAATAAAATCTTTCGATTATGATGCCTGGG
  CAAAACTTGATGTGGACAGTATTCTTGATGAGCTTGATAAAGAAGACAGCACCCACGATTCTGTGTCCCA
  GGAGTCAGAGTCGGACGAAGACGGTGTTCGTGTGGATTCCCAGAAGGCTCTTGTTCTAAAAGAAAAGGGC
  AACAAGTACTTCAAGCAAGGGAAATATGACGAAGCCATTGAATGCTACACGAAAGGCATGGATGCCGATC
  CCTACAACCCTGTGTTGCCAACAAACAGAGCATCTGCCTACTTTAGACTGAAAAAATTTGCTGTTGCTGA
  GTCTGACTGTAATTTGGCAATTGCCTTAAGCAGGAGTTATACCAAGGCTTATGCCAGACGAGGTGCTGCT
  CGATTTGCTCTGCAGAAGTTAGAGGACGCTAGAAAAGATTATGTAAAAGTGTTAGAACTGGAACCAGATA
  ACTTCGAAGCAACGAATGAACTCAGGAAAATTGATCAGGCTTTAACATCGAAGGAAAATTCACATCCCAA
  AGACATTGCTGCCGTGATTAAACCAGCTGAAGGAGAGAGAAAAGCAAATGAAGATCAGCGGGGCAGGCAG
  AAGGCCATTGCAGAGAAAGACCTGGGCAATGGATTTTTCAAAGAGGGGAAGTACGAGCAAGCAATCGAAT
  GCTACACCCGTGGGATAGCAGCGGACAGCACCAATGCCCTGCTCCCCGCTAACAGAGCCATGGCCTACCT
  CAAGGTTCAGAAATATGAAGAAGCTGAAAGAGACTGCACACAGGCTATCTTACTAGATGGTTCATATTCA
  AAAGCTTTTGCCAGAAGAGGAACTGCAAGGACATTTTTGGGAAAGATCAATGAGGCCAAGCAAGATTTCG
  AAACTGTTTTACTTCTGGAACCTGGAAATAAACAGGCAGTGACCGAGCTTTCCAGAATTAAAAAGGAATT
  AATTGAGAAAGGACGCTGGGATGATGTCTTTCTTGACTCCACACAGAGGCACAATGTGGTAAAGCCTGTA
  GACAGTCCTCATCGGGGGTCACCTAAAGCACTCAAGAAGGTTTTTATTGAAGAAACTGGCAATTTGATAG
  AGTCTGTCGATGCACCAGAGAGCTCTGCCACCGTCCCCGAGAGTGACCGAGCAGCAGTGGCTGTGGACAC
  TGGACGCAAGAAGGATTTCAGCCAGGGCGACTCTGTGTCCTCAGGGGAGACACCAAGAGCTAAAGTGTTG
  AAAATAGAAGCAGTCGGCGACTCGTCAGCCCCACAGGCGCAGGTGGACGTGAAGCAGGGCGTGCGTCAGT
  CAGTGAGTGAGAAAACCTCTGTGAGGGTAGCGCAGACGCCGGGTCAGTTGGCCGCTGTAGTTCTGCCTCC
  AGTTCCTGCCAACTCCTTCCAGCTGGAGTCTGATTTCAGACAGTTGAGAAGTTCCCCCGAGATGCTGTAT
  CAGTATGTGAAGAAAATTGAACCATCTTTGTATCCGAAGTTGTTTCAGAAAAACCTAGATCCAGATGTAT
  TCAATCAAATCATTAAAATTTTACACGACTTTTACGTTGAGAGGGAAAAGCCAGCTCTCATCTTTGAAGT
  CTTAGAAAGGCTTTCTCAGCTAAGAAGGTTCGACATGGCGGTGATGTTTATGTCAGGAACTGAGAGAGAG
  CTCACAAAAGTATTATTCAATCACTTAGAAAAATCAGAATTGAAGGAGGATTCTGTTGAAGAACTCAAGA
  AAAGATACGGCGGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201292 representing NM_001004243
  Red=Cloning site Green=Tags(s)
  
  MTSTSKAVELQLQVKHNAEELQDFMRDLEHWEKTMRQKDLELRRQSGVPEENLPPIRNGSFRKKKKRKTK
  DSSKKTKEENTKNRIKSFDYDAWAKLDVDSILDELDKEDSTHDSVSQESESDEDGVRVDSQKALVLKEKG
  NKYFKQGKYDEAIECYTKGMDADPYNPVLPTNRASAYFRLKKFAVAESDCNLAIALSRSYTKAYARRGAA
  RFALQKLEDARKDYVKVLELEPDNFEATNELRKIDQALTSKENSHPKDIAAVIKPAEGERKANEDQRGRQ
  KAIAEKDLGNGFFKEGKYEQAIECYTRGIAADSTNALLPANRAMAYLKVQKYEEAERDCTQAILLDGSYS
  KAFARRGTARTFLGKINEAKQDFETVLLLEPGNKQAVTELSRIKKELIEKGRWDDVFLDSTQRHNVVKPV
  DSPHRGSPKALKKVFIEETGNLIESVDAPESSATVPESDRAAVAVDTGRKKDFSQGDSVSSGETPRAKVL
  KIEAVGDSSAPQAQVDVKQGVRQSVSEKTSVRVAQTPGQLAAVVLPPVPANSFQLESDFRQLRSSPEMLY
  QYVKKIEPSLYPKLFQKNLDPDVFNQIIKILHDFYVEREKPALIFEVLERLSQLRRFDMAVMFMSGTERE
  LTKVLFNHLEKSELKEDSVEELKKRYGGG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004243
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004243.1, NP_001004243.1
• RefSeq Size: 2220 bp
• RefSeq ORF: 1980 bp
• Locus ID: 300189
• Cytogenetics: 7q36
• MW: 74.6 kDa