Sdha (NM_130428) Rat Tagged ORF Clone

CAT#: RR201347

Sdha (Myc-DDK-tagged ORF) - Rat succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (Sdha), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sdha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201347 representing NM_130428
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGTTGGCGCAGTTTCGAGACTTCTTCGCGGGCGGCGCTTGGCTCTAGCTGGGGCGACTCGTG
  GCTTTCACTTCTCTGTTGGTGAGAGCAAGAAGGCATCCGCTAAAGTTTCAGACGCGATTTCTACCCAGTA
  CCCCGTGGTGGACCATGAGTTTGATGCTGTGGTTGTAGGTGCAGGCGGGGCAGGCTTGCGAGCTGCATTC
  GGCCTTTCTGAGGCAGGCTTTAACACGGCATGCCTTACAAAGCTCTTTCCTACCCGCTCACATACTGTTG
  CAGCACAGGGAGGTATCAATGCTGCCCTGGGGAACATGGAAGAGGACAACTGGAGATGGCATTTCTATGA
  CACCGTGAAAGGCTCTGACTGGCTGGGGGATCAGGATGCCATCCATTACATGACAGAGCAAGCTCCTGCC
  TCCGTGGTTGAGCTAGAAAATTACGGTATGCCGTTTAGCAGGACTGAAGATGGGAGGATTTATCAGCGTG
  CATTTGGTGGACAGAGCCTCAAGTTCGGGAAAGGCGGGCAGGCCCATCGGTGTTGCTGTGTCGCTGATCG
  GACGGGCCACTCACTCTTACACACCTTGTATGGACGATCTCTGCGGTATGACACCAGTTATTTTGTGGAG
  TATTTCGCACTGGATCTTCTGATGGAAAATGGGGAGTGCCGTGGTGTCATTGCACTGTGCATAGAAGATG
  GGTCCATACACCGAATAAGAGCAAAGAACACTATTATTGCTACTGGGGGCTATGGGCGAACCTACTTCAG
  CTGTACTTCTGCCCACACCAGCACAGGGGACGGCACAGCCATGGTCACTCGGGCTGGTTTACCTTGCCAG
  GACTTAGAATTTGTTCAGTTCCACCCCACAGGTATCTATGGTGCTGGCTGCCTCATCACAGAAGGGTGCC
  GTGGAGAGGGAGGCATTCTCATCAACAGCCAAGGCGAAAGGTTCATGGAGAGATATGCCCCTGTTGCCAA
  GGACCTAGCATCAAGAGATGTTGTGTCTCGATCCATGACTCTCGAGATCCGTGAAGGAAGAGGCTGTGGC
  CCTGAGAAGGATCACGTCTACCTGCAGTTGCACCATCTGCCCCCTGAGCAGCTGGCCACGCGTCTGCCTG
  GGATCTCAGAGACGGCCATGATCTTCGCCGGCGTGGATGTCACCAAGGAGCCCATTCCAGTCCTTCCCAC
  TGTGCATTACAACATGGGCGGGATTCCCACTAACTACAAGGGACAGGTGCTGAAGCACGTGAACGGCCAG
  GATCAGATTGTGCCTGGTCTGTACGCCTGTGGGGAGGCTGCCTGCGCCTCAGTGCATGGTGCCAACCGGC
  TTGGAGCAAACTCTCTTTTGGACCTTGTCGTCTTTGGCCGAGCCTGTGCCCTGAGCATTGCAGAATCTTG
  CAGGCCTGGAGATAAAGTTCCTCCGATTAAGGCAAATGCTGGAGAAGAGTCGGTTATGAATCTTGACAAG
  TTGAGATTTGCTGATGGAAGTGTAAGAACATCAGAGCTGCGCCTCAGCATGCAGAAGTCGATGCAGAGCC
  ATGCCGCCGTGTTCCGTGTGGGAAGTGTGCTGCAAGAAGGCTGTGAAAAAGTCAGCCAGCTCTATGGAGA
  CCTACAGCATCTGAAGACGTTTGACAGGGGAATGGTCTGGAACACAGACCTGGTGGAGACGCTGGAGCTG
  CAGAATCTGATGCTGTGCGCACTGCAGACCATATATGGTGCGGAAGCACGGAAGGAGTCACGGGGAGCTC
  ATGCCAGGGAAGATTACAAGGTGCGGATTGATGAGTATGATTACTCCAAGCCCATCGAGGGCCAGCAGAA
  GAAGCCATTTGCGGAACACTGGAGGAAGCACACCCTCTCATATGTGGACACCAAGACTGGGAAGGTTACT
  TTGGATTACAGACCTGTTATTGACAAGACCTTGAATGAGGCTGACTGTGCTACTGTACCTCCTGCTATCC
  GTTCCTAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201347 representing NM_130428
  Red=Cloning site Green=Tags(s)
  
  MAGVGAVSRLLRGRRLALAGATRGFHFSVGESKKASAKVSDAISTQYPVVDHEFDAVVVGAGGAGLRAAF
  GLSEAGFNTACLTKLFPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIHYMTEQAPA
  SVVELENYGMPFSRTEDGRIYQRAFGGQSLKFGKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVE
  YFALDLLMENGECRGVIALCIEDGSIHRIRAKNTIIATGGYGRTYFSCTSAHTSTGDGTAMVTRAGLPCQ
  DLEFVQFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCG
  PEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLKHVNGQ
  DQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIAESCRPGDKVPPIKANAGEESVMNLDK
  LRFADGSVRTSELRLSMQKSMQSHAAVFRVGSVLQEGCEKVSQLYGDLQHLKTFDRGMVWNTDLVETLEL
  QNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIEGQQKKPFAEHWRKHTLSYVDTKTGKVT
  LDYRPVIDKTLNEADCATVPPAIRSY
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130428
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_130428.1, NP_569112.1
• RefSeq Size: 2277
• RefSeq ORF: 1971
• Locus ID: 157074
• MW: 71.6 kDa
• Gene Summary: subunit of the mitochondrial succinate hehydrogenase that is involved in the oxidation of succinate; mutation of human homolog is associated with Leigh syndrome [RGD, Feb 2006]