Gclc (NM_012815) Rat Tagged ORF Clone

CAT#: RR201392

Gclc (Myc-DDK-tagged ORF) - Rat glutamate-cysteine ligase, catalytic subunit (Gclc), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gclc
• Synonyms: Glclc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201392 representing NM_012815
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCTGTCCCAAGGCTCGCCACTGAGCTGGGAAGAGACCCAGCGCCACGCCGACCACGTGCGGA
  GACACGGCATCCTCCAGTTCCTGCACATCTACCACGCAGTCAAGGACCGGCACAAGGACGTGCTCAAGTG
  GGGTGACGAGGTGGAGTACATGTTGGTGTCCTTTGATCATGAAAATAGGAAAGTCCAGTTGTTACTGAAT
  GGCGGCGATGTTCTTGAAACTCTGCAAGAGAAGGGGGAGAGGACAAACCCCAACCACCCAACCCTCTGGA
  GACCAGAGTATGGGAGTTACATGATTGAAGGGACACCTGGCCAGCCGTACGGAGGAACGATGTCCGAGTT
  CAACACAGTGGAGGACAACATGAGGAAACGCCGGAAGGAGGCTACTTCTGTATTAGGAGAACATCAGGCT
  CTTTGCACGATAACTTCATTTCCCAGGCTAGGCTGCCCTGGATTCACACTGCCAGAGCACAGACCCAACC
  CAGAGGAAGGAGGTGCATCTAAGTCCCTCTTCTTTCCAGACGAAGCCATAAACAAGCACCCCCGCTTTGG
  TACTCTAACAAGAAACATCCGGCATCGGAGAGGAGAAAAGGTTGTCATCAATGTGCCAATATTCAAGGAC
  AAGAACACACCATCTCCGTTTGTAGAAACATTTCCTGAGGATGAGGAGGCATCAAAGGCCTCTAAGCCAG
  ACCACATCTACATGGATGCCATGGGATTTGGGATGGGCAACTGCTGTCTTCAGGTGACATTCCAAGCCTG
  CAGTATATCTGAGGCAAGATACCTTTATGACCAGTTGGCCACTATCTGCCCAATTGTTATGGCTTTGAGT
  GCTGCATCGCCATTTTACCGAGGCTACGTGTCAGACATTGATTGTCGCTGGGGAGTGATTTCTGCATCTG
  TAGATGATAGAACACGGGAGGAGAGAGGACTGGAGCCCCTGAAGAACAATCGCTTTAAAATCAGTAAGTC
  TCGGTATGACTCAATAGATAGCTACCTGTCCAAGTGTGGAGAGAAGTACAATGACATCGACCTGACCATC
  GACACGGAGATCTACGAGCAGCTCTTAGAGGAAGGCATCGATCACCTTCTGGCACAGCACGTTGCTCATC
  TCTTTATTAGAGACCCACTGACCCTTTTTGAAGAGAAAATTCATCTGGATGATGCCAACGAGTCTGACCA
  TTTTGAGAATATTCAGTCCACAAACTGGCAGACAATGAGGTTTAAGCCTCCTCCTCCAAACTCAGATATT
  GGATGGAGAGTAGAGTTCCGACCAATGGAGGTACAGTTGACAGACTTTGAGAACTCTGCCTATGTGGTAT
  TTGTGGTACTGCTGACCAGGGTGATCCTCTCATACAAACTAGACTTCCTCATTCCACTGTCCAAGGTTGA
  CGAGAACATGAAAGTGGCACAGGAGCGAGATGCCGTCTTACAGGGGATGTTTTATTTCAGGAAAGACATT
  TGCAAAGGTGGCAACGCCGTGGTGGATGGGTGTAGCAAGGCCCAGACCAGCTCCGAGCCATCTGCAGAGG
  AGTACACGCTCATGAGCATAGACACCATCATCAATGGGAAGGAAGGCGTGTTTCCTGGACTCATCCCCAT
  TCTGAACTCCTACCTTGAAAACATGGAAGTCGACGTGGACACCCGATGCAGTATTCTGAACTACCTGAAG
  CTAATTAAGAAGAGAGCATCTGGAGAACTAATGACTGTTGCCAGGTGGATGAGAGAGTTTATTGCAAACC
  ATCCTGACTACAAGCAAGACAGTGTAATAACTGATGAGATCAACTATAGCCTCATTTTGAAATGCAATCA
  AATTGCAAATGAATTGTGTGAATGTCCAGAGTTACTTGGATCAGGCTTTAGAAAAGCGAAGTACAGTGGA
  GGTAAAAGCGACCCTTCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201392 representing NM_012815
  Red=Cloning site Green=Tags(s)
  
  MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENRKVQLLLN
  GGDVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSVLGEHQA
  LCTITSFPRLGCPGFTLPEHRPNPEEGGASKSLFFPDEAINKHPRFGTLTRNIRHRRGEKVVINVPIFKD
  KNTPSPFVETFPEDEEASKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALS
  AASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNRFKISKSRYDSIDSYLSKCGEKYNDIDLTI
  DTEIYEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDI
  GWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQERDAVLQGMFYFRKDI
  CKGGNAVVDGCSKAQTSSEPSAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLK
  LIKKRASGELMTVARWMREFIANHPDYKQDSVITDEINYSLILKCNQIANELCECPELLGSGFRKAKYSG
  GKSDPSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012815
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012815.1, NM_012815.2, NP_036947.1
• RefSeq Size: 3079
• RefSeq ORF: 1914
• Locus ID: 25283
• MW: 72.6 kDa
• Gene Summary: catalyzes the first step in the synthesis of glutathione [RGD, Feb 2006]