Ddhd1 (NM_001033066) Rat Tagged ORF Clone

CAT#: RR201394

Ddhd1 (Myc-DDK-tagged ORF) - Rat DDHD domain containing 1 (Ddhd1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddhd1
• Synonyms: MGC125147
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201394 representing NM_001033066
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTACCCCGGCCGCGGCGCCCCACGGAGCCCCGAGCGTAACGGCCGCGGCGGCGGTGCCTGGGAGC
  TAGGCTCGGACGCGGCGGGCGTGTTCGGTGGCGGCGGCTGCTGCTTCGAGCACCAGCCGGGTGACGGCGT
  GCCGCTGTCACTGCTGCGCGCGGAGCCGCTGCACCTGGCACCGGGCGCGGACGACTTGAGCCACCTGGCT
  CTGGACCCGTGCCTCAGCGACGAGAACTACGACTTCAGCTCGGCCGAGTCGGGTTCGTCGCTGCGCTACT
  ACAGCGAGGGCGAGAGCGCGGGCGGCGGTAGCTCTTCTTCGCCTCCGCCGCCGCTGGTCGCTCCGAACTC
  GGGGGGCGGCGGGGCAGCGGGAGGGGGACCCGGTGACAGGAAGCGCTCCCGGCCGGGCGGCTGCGCCGCT
  CGGCACCGCTACGAGGTAGTGACGGAGCTGGGCCCCGAGGAGGTGCGCTGGTTCTACAAGGAAGACAAGA
  AGACCTGGAAGCCCTTCATCGGCTACGACTCTCTGCGCATTGAGCTCGCCTTCCGGAGCCTCCTGCAGAC
  GACGGGCTCTCGTGCCCGGTCGGCGGACCGCGATGGAGACCGGGTGTGCGGCCCAACCTCTAGCTTCAGG
  GAGGACGACGAGGACCGCGCCTGCGGCTTCTGCCCGCGCGCGGTGGGGCCTGAGCCGGAGATGGAGGAGC
  TGGTGACCATCGAGCCCGTGTGCGTGCGCGGCGGCCTCTACGAGGTGGATGTCACTCAAGGAGAGTGCTA
  CCCGGTATACTGGAACCAGGCCGATAAAATACCAGTGATGCGTGGACAGTGGTTTATCGATGGCACTTGG
  CAACCTCTGGAGGAAGAAGAGAGTAACTTAATTGAGCAAGAACATCTCAGTTGTTTTAGGGGTCAGCAGA
  TGCAAGAGAATTTTGATATTGAAATGTCCAAATCCATCGATGGAAAAGACGCTGTTCATAGTTTCAAACT
  GAGTCGGAACCACGTGGACTGGCACAGCATGGACGAGGTCTATCTCTACAGTGACGCAACAACTTCCAAA
  ATTGCAAGAACTGTTACCCAGAAACTGGGGTTTTCTAAAGCCTCCAGCAGTGGGACCAGACTTCACAGAG
  GCTACGTAGAAGAAGCCACACTAGAAGACAAGCCATCGCAAACTTCTCATATTGTGTTTGTTGTGCACGG
  CATTGGACAGAAGATGGACCAAGGAAGAATCATCAAAAACACAGCCATGATGAGAGAGGCTGCAAGGAAA
  ATAGAAGAAAAGCATTTTGCCAACCACGCAACACACGTTGAGTTTCTACCTGTGGAGTGGCGATCGAAGC
  TTACTCTTGATGGAGACACTGTTGACTCCATCACTCCTGACAAGGTGCGGGGCCTGAGGGACATGCTCAA
  CAGCAGCGCCATGGACATCATGTACTACACCAGCCCGCTCTACAGGGATGAGCTAGTTAAAGGCCTTCAG
  CAAGAGCTGAATCGATTATATTCCCTTTTCTGTTCCCGGAATCCAGACTTTGAAGAGAAAGGGGGTAAAG
  TCTCAATAGTGTCCCATTCCTTGGGGTGTGTAATCACTTATGACATCATGATGGGCTGGAATCCAGTTCG
  ACTTTATGAGCAGCTGCTGCAGAAGGAGGAGGAGCTGCCTGACGAGCGGTGGATGAGCTACGAAGAGCGG
  CATCTTCTTGATGAGCTCTATATCACTAAACGGCGGCTGAGGGAAATTGAAGATCGGCTGCATGGATTGA
  AGGCGCCATCCGGCTCACAAACACCTGCCTTAAAGTTTAAGGTTGAGAATTTCTTCTGTATGGGATCTCC
  GCTAGCAGTTTTTTTAGCATTACGCGGCATCCGACCAGGAAATACCGGAAGTCAAGACCACATTTTACCT
  AGAGAGATCTGTAACCGCTTACTAAATATTTTTCATCCTACAGACCCAGTGGCCTACAGATTAGAGCCAT
  TGATTCTGAAACACTACAGCAACATTTCTCCAGTCCAGATCCACTGGTACAACACTTCGAACCCTCTACC
  TTATGAGCATATGAAGCCAAACTTTCTCAACCCAGCAAAAGAGCCTACCTCAGCTTCAGAGAGTGAGAAC
  ATCGCAGCTATCCCGAGCCCTGTAACCTCCCCAGTCTTATCTCGGCGCCACTATGGGGAATCCATAACTA
  ACATTGGCAAAGCGAGCATACTGGGGGCTGCTAGCATTGGAAAGGGGCTTGGAGGAATGTTGTTCTCAAG
  GTTCGGACGGTCCTCCACACCACAGCCGTCGGAGCCATCTAAGGACTCAGCGGAAGAGGAGAAGAAGCCT
  GTCTCTTCACCCTCCACCACCACCGTGGCCACGCAGACCCTGCCGCACAGCAGCTCCGGCTTCCTCGACT
  CTGCATTGGAACTGGAGCACAGGATCGACTTCGAACTCAGAGAAGGCCTTGTGGAGAGCCGCTATTGGTC
  AGCCGTCACGTCGCACACTGCCTATTGGTCATCCTTGGATGTCGCTCTTTTTCTTTTAACATTCATGTAC
  AAGCACGAGCATGACAGCGAGGCAAAGACCAGCCTAGATTCAATCAAGCACGAGCATGACAGCGAGGCAA
  AGACCAGCCTAGATTCAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201394 representing NM_001033066
  Red=Cloning site Green=Tags(s)
  
  MNYPGRGAPRSPERNGRGGGAWELGSDAAGVFGGGGCCFEHQPGDGVPLSLLRAEPLHLAPGADDLSHLA
  LDPCLSDENYDFSSAESGSSLRYYSEGESAGGGSSSSPPPPLVAPNSGGGGAAGGGPGDRKRSRPGGCAA
  RHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRSLLQTTGSRARSADRDGDRVCGPTSSFR
  EDDEDRACGFCPRAVGPEPEMEELVTIEPVCVRGGLYEVDVTQGECYPVYWNQADKIPVMRGQWFIDGTW
  QPLEEEESNLIEQEHLSCFRGQQMQENFDIEMSKSIDGKDAVHSFKLSRNHVDWHSMDEVYLYSDATTSK
  IARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTSHIVFVVHGIGQKMDQGRIIKNTAMMREAARK
  IEEKHFANHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQ
  QELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMMGWNPVRLYEQLLQKEEELPDERWMSYEER
  HLLDELYITKRRLREIEDRLHGLKAPSGSQTPALKFKVENFFCMGSPLAVFLALRGIRPGNTGSQDHILP
  REICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPNFLNPAKEPTSASESEN
  IAAIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRSSTPQPSEPSKDSAEEEKKP
  VSSPSTTTVATQTLPHSSSGFLDSALELEHRIDFELREGLVESRYWSAVTSHTAYWSSLDVALFLLTFMY
  KHEHDSEAKTSLDSIKHEHDSEAKTSLDSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033066
• ORF Size: 2565 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3432 bp
• RefSeq ORF: 2568 bp
• Locus ID: 305816
• Cytogenetics: 15p14
• MW: 95.2 kDa