Uqcrc1 (NM_001004250) Rat Tagged ORF Clone

CAT#: RR201407

Uqcrc1 (Myc-DDK-tagged ORF) - Rat ubiquinol-cytochrome c reductase core protein I (Uqcrc1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uqcrc1
• Synonyms: MGC93712
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201407 representing NM_001004250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCCGCAGTCTGCCGAGCGGCCTGCTCTGGGACGCAAGCGCTACTTCGCACCTGCCGCTCGC
  CGGCCCTGCTGAGGCTACCTGCCTTGCGGGGTACCGCAACCTTCGTCCAGGCCCTCCAGAGCGTGCCGGA
  GACACAGGTCAGCGTATTGGACAACGGGCTGCGTGTGGCCTCGGAGCAGTCCTCGCATCCTACCTGCACG
  GTGGGAGTGTGGATTGACGTTGGCAGTCGCTATGAGACTGAGAAGAACAATGGAGCAGGCTACTTTTTGG
  AACATCTGGCTTTCAAGGGAACAAAGAACCGTCCTGGCAATGCCTTAGAGAAGGAGGTAGAGAGTATTGG
  CGCCCATCTTAATGCCTACAGCACTCGGGAGCACACTGCTTACCTCATCAAGGCACTGTCCAAGGACCTG
  CCAAAAGTTGTGGAGCTCCTGGCAGATATTGTGCAGAACATTAGTCTGGAAGACTCGCAGATCGAGAAGG
  AGCGAGATGTGATCCTGCGGGAGATGCAGGAAAACGATGCCTCTATGCAGAATGTAGTCTTCGATTACCT
  GCATGCAACAGCATTCCAGGGCACACCTCTAGCCCAGGCTGTGGAGGGGCCCAGTGAGAACGTCAGGAGG
  CTGTCGCGCACAGACTTGACTGACTACCTCAGCAGGCACTACAAAGCTCCCCGAATGGTGCTGGCTGCCG
  CTGGAGGGGTGAAGCACCAGCAGCTGCTAGACCTTGCCCAGGATCACTTCAGCAGTGTCTCCCAGGTGTA
  TGAAGAAGACGCTGTACCTAGTATAACTCCATGTCGCTTCACTGGCAGTGAGATCCGACATCGGGATGAT
  GCCCTGCCCTTGGCCCATGTGGCCATTGCAGTAGAGGGACCTGGCTGGGCTAACCCAGACAACGTGGCTC
  TCCAAGTGGCCAATGCCATCATAGGCCACTATGACTGCACTTACGGTGGTGGAGTGCACCTGTCCAGCCC
  GCTGGCTTCCGTTGCTGTAGCTAACAAGCTTTGCCAGAGTTTCCAGACCTTCAACATCTCCTACTCTGAG
  ACCGGGTTGCTGGGCGCACACTTTGTCTGTGATGCCATGAGCATCGATGACATGATCTTCTTCCTGCAAG
  GCCAGTGGATGCGCCTGTGCACCAGCGCTACAGAGAGTGAGGTGACTCGGGGCAAAAACATCCTTAGGAA
  TGCCTTGATATCTCATTTGGATGGCACCACCCCTGTGTGTGAAGACATTGGTCGCAGCCTCCTGACTTAT
  GGCCGCCGCATCCCTCTGGCTGAGTGGGAGAGCCGGATTGAGGAGGTGGATGCCCAGATGGTTCGAGAGG
  TTTGCTCCAAGTACTTTTATGACCAATGTCCAGCAGTGGCTGGATATGGCCCCATCGAACAGCTCTCAGA
  CTACAACCGGATTCGCAGTGGCATGTTCTGGCTGCGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201407 representing NM_001004250
  Red=Cloning site Green=Tags(s)
  
  MAASAVCRAACSGTQALLRTCRSPALLRLPALRGTATFVQALQSVPETQVSVLDNGLRVASEQSSHPTCT
  VGVWIDVGSRYETEKNNGAGYFLEHLAFKGTKNRPGNALEKEVESIGAHLNAYSTREHTAYLIKALSKDL
  PKVVELLADIVQNISLEDSQIEKERDVILREMQENDASMQNVVFDYLHATAFQGTPLAQAVEGPSENVRR
  LSRTDLTDYLSRHYKAPRMVLAAAGGVKHQQLLDLAQDHFSSVSQVYEEDAVPSITPCRFTGSEIRHRDD
  ALPLAHVAIAVEGPGWANPDNVALQVANAIIGHYDCTYGGGVHLSSPLASVAVANKLCQSFQTFNISYSE
  TGLLGAHFVCDAMSIDDMIFFLQGQWMRLCTSATESEVTRGKNILRNALISHLDGTTPVCEDIGRSLLTY
  GRRIPLAEWESRIEEVDAQMVREVCSKYFYDQCPAVAGYGPIEQLSDYNRIRSGMFWLRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004250
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004250.1, NM_001004250.2, NP_001004250.1
• RefSeq Size: 1597 bp
• RefSeq ORF: 1443 bp
• Locus ID: 301011
• Cytogenetics: 8q32
• MW: 53.3 kDa
• Gene Summary: human homolog catalyzes the transfer of electrons from coenzyme QH2 to ferricytochrome c in mitochondrial electron transport [RGD, Feb 2006]