Slit3 (NM_031321) Rat Tagged ORF Clone

CAT#: RR201491

Slit3 (Myc-DDK-tagged ORF) - Rat slit homolog 3 (Drosophila) (Slit3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slit3
• Synonyms: Megf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201491 representing NM_031321
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCGGGCGGACCGGGGCCGGCGCCGCTGTGCGCGCCCGCCTGGCGCTGGCCTTGGCGCTGGCGA
  GCATCCTGAGCGGACCCCCCGCTGCTGCCTGCCCCACCAAGTGTACCTGCTCCGCCGCTAGCGTGGACTG
  CCACGGGCTGGGCCTGCGCGCCGTTCCCCGGGGCATCCCCCGCAACGCTGAGCGCCTTGACCTGGACAGA
  AATAACATCACCAGGATCACCAAAATGGACTTCACCGGCTTGAAGAATCTCCGAGTCTTGCATCTGGAAG
  ACAATCAGGTCAGCGTCATCGAGAGAGGCGCCTTCCAGGATCTGAAGCAGCTGGAGCGATTACGTCTGAA
  CAAGAACAAGCTCCAGGTCCTTCCAGAATTACTTTTCCAGAGCACACCGAAGCTCACCAGACTAGATCTG
  AGCGAAAACCAGATCCAGGGCATCCCGAGGAAGGCGTTCAGGGGCGTCACGGGCGTGAAGAACCTGCAAC
  TGGACAATAACCACATCAGCTGCATTGAAGATGGAGCCTTCCGAGCGCTGCGCGATTTGGAGATCCTCAC
  CCTTAACAACAACAACATCAGCCGTATCCTGGTCACCAGCTTCAACCACATGCCGAAGATCCGGACCCTG
  CGTCTCCACTCCAACCACCTGTACTGTGATTGTCACTTGGCCTGGCTCTCAGACTGGCTGCGACAGCGGC
  GGACCATTGGCCAGTTCACCCTCTGCATGGCGCCCGTGCACCTGAGAGGCTTCAGTGTAGCAGACGTGCA
  GAAGAAGGAGTATGTGTGTCCAGGTCCCCACTCAGAGGCTCCAGCCTGCAATGCCAACTCCCTCTCCTGC
  CCTTCTGCCTGCTCATGCAGTAATAACATTGTAGACTGCCGTGGGAAGGGACTGACTGAGATCCCTGCCA
  ACCTGCCGGAGGGCATCGTGGAAATACGCCTAGAACAGAACTCCATCAAATCCATTCCTGCAGGAGCTTT
  CATCCAGTACAAAAAACTGAAGCGAATAGACATCAGCAAGAATCAGATATCGGACATTGCTCCAGATGCC
  TTCCAGGGCCTGAAATCACTCACGTCGCTGGTGCTCTATGGGAACAAGATCACAGAGATTCCCAAAGGAC
  TGTTTGACGGGCTGGTGTCCCTGCAGCTGCTCCTCCTCAATGCCAACAAGATCAACTGCCTGCGGGTAAA
  CACCTTCCAGGACCTACAGAACCTCAATCTGCTCTCTCTGTATGACAACAAGTTGCAGACTATCAGCAAA
  GGGCTCTTTGCTCCGCTGCAGTCCATCCAGACCCTCCACTTAGCTCAAAACCCGTTTGTTTGCGACTGCC
  ACTTGAAGTGGTTGGCCGACTACCTCCAGGACAACCCCATTGAGACGAGCGGGGCCCGCTGCAGCAGCCC
  ACGCCGGCTGGCCAACAAGCGCATCAGCCAGATCAAAAGCAAGAAGTTCCGCTGCTCAGGCTCGGAGGAT
  TATCGCAACAGATTCAGCAGCGAGTGCTTCATGGACCTAGTGTGCCCCGAGAAGTGCCGTTGTGAGGGCA
  CCATTGTGGACTGCTCCAACCAGAAGCTCTCCCGCATCCCGAGCCACCTCCCTGAATATACCACTGACCT
  GCGACTGAACGACAATGACATCGCTGTGCTGGAGGCCACTGGGATCTTCAAGAAGTTGCCCAACCTGAGG
  AAAATAAACTTGAGCAATAATAGGATCAAGGAGGTGCGGGAGGGTGCGTTTGATGGAGCGGCAGGCGTGC
  AGGAACTGATGCTGACGGGGAACCAGCTGGAGACCATGCACGGACGCATGTTCCGGGGCCTCAGCGGCCT
  GAAGACACTGATGTTAAGGAGCAACCTGATCAGCTGCGTAAACAATGACACCTTCGCTGGCCTAAGCTCT
  GTGAGACTGCTGTCCCTCTATGACAATCGGATCACCACCATCAGCCCTGGAGCCTTCACCACGCTCGTCT
  CCCTGTCCACCATAAACCTCTTGTCTAACCCTTTCAACTGCAACTGTCACATGGCCTGGCTCGGCCGGTG
  GCTGAGGAAACGCCGCATCGTAAGCGGGAACCCCAGATGTCAGAAGCCCTTTTTCCTCAAGGAGATTCCT
  ATCCAAGATGTGGCCATCCAGGACTTCACCTGTGAAGGCAATGAAGAGAACAGCTGTCAGCTGAGTCCAC
  GCTGCCCCGAGCAGTGTACCTGTGTGGAGACAGTGGTGCGATGCAGCAACAGGGGTCTCCACACCCTCCC
  CAAGGGCATGCCAAAGGACGTGACTGAACTGTACCTGGAAGGAAATCATTTAACGGCGGTGCCCAAAGAA
  TTGTCCACCTTCCGACAGCTGACACTAATTGACCTGAGCAACAACAGCATCAGTATGCTGACCAATCACA
  CCTTCAGCAACATGTCCCACCTCTCCACACTGATCCTGAGCTACAACCGGCTGAGATGCATCCCGGTCCA
  TGCCTTCAACGGGCTAAGGTCACTCCGAGTGCTAACCCTCCATGGCAATGACATTTCCAGTGTTCCTGAA
  GGCTCCTTCAATGATCTGACGTCCCTCTCCCACCTGGCCCTGGGAATCAACCCTCTCCACTGTGACTGCA
  GTCTGCGTTGGTTATCAGAGTGGATAAAGGCTGGGTACAAGGAGCCTGGCATTGCCAGATGCAGTAGCCC
  CGAGTCCATGGCAGACAGACTCCTGCTCACCACCCCCACCCACAGGTTCCAGTGCAAAGGGCCAGTGGAC
  ATTAACATCGTGGCCAAGTGCAACGCCTGCCTCTCTAGCCCATGCAAGAACAACGGCACTTGCAGCCAGG
  ATCCGGTGGAGCAGTACCGCTGTACCTGCCCTTACAGCTACAAGGGCAAGGACTGCACCGTGCCCATCAA
  CACCTGCGTCCAGAACCCCTGTCAGCACGGAGGCACCTGTCACCTCAGTGAGAGCCACAGAGATGGGTTC
  AGCTGCTCCTGCCCCCTGGGCTTTGAGGGACAGCGGTGTGAGATCAACCCAGATGACTGTGAGGATAACG
  ACTGTGAAAACAGCGCCACCTGTGTGGACGGGATCAACAACTACGCATGCGTGTGCCCGCCGAACTACAC
  AGGGGAGCTGTGTGATGAGGTGATTGACTACTGCGTGCCCGAGATGAACCTCTGTCAGCACGAGGCCAAG
  TGCATCTCCCTGGACAAAGGATTCAGGTGCGAATGTGTCCCTGGCTACAGTGGAAAGCTGTGCGAGACAG
  ACAACGATGACTGTGTGGCTCACAAGTGTCGCCACGGAGCCCAGTGTGTGGACGCGGTCAATGGCTACAC
  GTGCATCTGCCCCCAGGGCTTCAGCGGGCTCTTCTGTGAGCACCCCCCACCCATGGTTCTGCTACAGACC
  AGCCCCTGTGACCAGTACGAGTGCCAGAATGGGGCGCAGTGCATCGTGGTACAGCAGGAGCCCACTTGCC
  GCTGTCCCCCAGGCTTCGCTGGGCCCAGGTGTGAGAAGCTCATCACTGTGAATTTCGTGGGCAAGGACTC
  CTATGTGGAACTGGCCTCTGCCAAGGTCCGGCCCCAGGCCAACATCTCCCTGCAGGTGGCCACTGACAAG
  GACAACGGCATCCTTCTTTACAAGGGAGACAACGACCCCCTGGCACTGGAGCTGTACCAGGGTCACGTGA
  GGCTGGTGTATGACAGCCTGAGCTCCCCTCCGACCACGGTGTACAGTGTGGAGACGGTGAATGATGGCCA
  GTTTCACAGTGTGGAGCTGGTGATGCTAAACCAGACCCTGAACCTGGTGGTAGACAAAGGAGCCCCCAAG
  AGCCTGGGGAAGCTCCAGAAGCAGCCAGCAGTGGGCATCAACAGTCCCCTCTATCTTGGAGGCATCCCCA
  CGTCTACAGGCCTCTCAGCCTTACGCCAGGGTGCAGACAGGCCGCTGGGCGGCTTCCACGGCTGTATACA
  CGAAGTGCGCATCAACAACGAGTTGCAGGATTTCAAAGCCCTCCCACCCCAGTCCCTGGGGGTCTCTCCC
  GGCTGCAAATCCTGCACTGTGTGTCGTCACGGCCTGTGTCGTTCCGTGGAGAAGGACAGCGTAGTGTGTG
  AGTGCCATCCGGGATGGACTGGTCCACTGTGTGATCAGGAAGCCCAGGACCCCTGCCTTGGTCACAGCTG
  CAGCCATGGGACATGCGTGGCGACTGGCAACTCATATGTGTGCAAGTGTGCCGAGGGCTATGAGGGACCT
  TTGTGTGACCAGAAGAATGACTCTGCCAATGCCTGCTCAGCCTTCAAGTGCCACCACGGGCAGTGCCACA
  TCTCAGATCGAGGGGAGCCCTATTGCCTGTGCCAGCCTGGCTTCAGTGGCAATCACTGTGAGCAAGAGAA
  TCCATGTTTGGGAGAGATAGTCCGTGAAGCCATCCGCCGCCAGAAAGATTATGCCTCCTGTGCCACAGCA
  TCCAAGGTGCCCATCATGGTATGCCGCGGGGGCTGCGGGAGCCAATGCTGCCAGCCGATTCGGAGCAAGC
  GGCGGAAATACGTCTTCCAGTGCACTGACGGCTCCTCGTTCGTGGAAGAGGTGGAGAGACACTTGGAATG
  TGGCTGTCGTGAGTGTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201491 representing NM_031321
  Red=Cloning site Green=Tags(s)
  
  MAPGRTGAGAAVRARLALALALASILSGPPAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDR
  NNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDL
  SENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTL
  RLHSNHLYCDCHLAWLSDWLRQRRTIGQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSC
  PSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDA
  FQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISK
  GLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSED
  YRNRFSSECFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLR
  KINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSS
  VRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIP
  IQDVAIQDFTCEGNEENSCQLSPRCPEQCTCVETVVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAVPKE
  LSTFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPE
  GSFNDLTSLSHLALGINPLHCDCSLRWLSEWIKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPVD
  INIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCQHGGTCHLSESHRDGF
  SCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACVCPPNYTGELCDEVIDYCVPEMNLCQHEAK
  CISLDKGFRCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDAVNGYTCICPQGFSGLFCEHPPPMVLLQT
  SPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDK
  DNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPK
  SLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSP
  GCKSCTVCRHGLCRSVEKDSVVCECHPGWTGPLCDQEAQDPCLGHSCSHGTCVATGNSYVCKCAEGYEGP
  LCDQKNDSANACSAFKCHHGQCHISDRGEPYCLCQPGFSGNHCEQENPCLGEIVREAIRRQKDYASCATA
  SKVPIMVCRGGCGSQCCQPIRSKRRKYVFQCTDGSSFVEEVERHLECGCRECS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031321
• ORF Size: 4569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031321.1, NP_112611.1
• RefSeq Size: 5210
• RefSeq ORF: 4572
• Locus ID: 83467
• MW: 167.8 kDa
• Gene Summary: localized to mitochondria; has less conservation with Drosophila slit than family members slit1 and slit2; may play a role in neurogenesis [RGD, Feb 2006]