Slc12a4 (NM_019229) Rat Tagged ORF Clone

CAT#: RR201621

Slc12a4 (Myc-DDK-tagged ORF) - Rat solute carrier family 12 (potassium/chloride transporters), member 4 (Slc12a4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc12a4
• Synonyms: Kcc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201621 representing NM_019229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCACTTCACCGTGGTGCCGGTGGACGGGCCGCGACGCGGCGACTATGACAACCTCGAGGGGCTCA
  GTTGGGTGGACTACGGGGAGCGCGCCGAGCGGGAAGACTCGGATGGACAGGGTAACCACAGAGAGAATAG
  TCCCTTCCTTAGCCCTTTGGACGCCTCCAGAGGAAATGACTACTATGACCGGAACCTGGCACTGTTTGAG
  GAGGAGCTGGACATCCGCCCAAAGGTATCATCTCTCCTGGGCAAGCTTGTCAGCTATACCAACCTCACCC
  AAGGAGCCAAGGAGCACGAGGAAGCTGAGAGTGGAGAAGGTGGCCGTCGGAGAGCCGCCAAGGCACCCAG
  CATGGGCACCCTTATGGGAGTGTACCTGCCCTGCCTGCAGAATATCTTCGGGGTCATCCTCTTCCTGCGG
  CTGACCTGGATGGTGGGCACAGCTGGCGTGCTGCAGGCTCTCCTCATTGTCCTCATCTGTTGCTGCTGTA
  CCCTGCTGACAGCCATCTCCATGAGTGCCATCGCCACCAATGGTGTGGTTCCAGCTGGTGGCTCTTACTT
  CATGATTTCCCGCTCTTTGGGACCAGAATTCGGAGGTGCTGTGGGCCTATGCTTCTACCTGGGGACCACA
  TTTGCAGCAGCCATGTATATCCTAGGAGCCATTGAGATCTTGCTGACCTACATTGCTCCACCAGCTGCCA
  TCTTTTACCCATCGGGCACACACGACATGTCAAGCGCCACCTTGAATAACATGCGGGTGTACGGAACCAT
  TTTCCTGACTTTCATGACCCTAGTGGTGTTTGTCGGTGTCAAGTATGTGAACAAGTTCGCCTCACTCTTC
  CTGGCCTGTGTGATCATCTCCATCCTCTCCATTTACGTGGGAGGCATCAAGTCCGCTTTTGACCCTCCTG
  TTTTTCCGGTGTGCATGCTGGGCAATAGGACTCTGTCTCGGGACCAGTTTGACATCTGTGCCAAGACAGT
  TGTGGTGGACAATGAGACAGTGGCCACCCGGCTGTGGACTTTCTTCTGCCACAGCCCCAACCTTACTGCT
  GACTCCTGTGACCCCTACTTCCTGCTCAACAATGTGACAGAGATTCCTGGCATACCTGGGGCAGCTGCTG
  GTGTGCTCCAGGAAAACCTGTGGAGTGCTTACCTGGAGAAGGGTGAGGTTGTGGAGAAGCATGGGCTGCC
  CTCCACAGATACCCTTGGCCTGAAGGAGAGCCTGTCCCTGTATGTGGTGGCCGACATCGCCACATCCTTC
  ACCGTGCTGGTTGGCATCTTTTTCCCTTCTGTAACAGGCATCATGGCTGGCTCAAACCGTTCCGGGGACC
  TCCGTGATGCTCAGAAGTCTATCCCTGTGGGGACCATTCTGGCTATTGTCACCACTTCACTCGTGTACTT
  CAGCAGTGTGATTCTCTTCGGTGCCTGCATCGAGGGTGTGGTGCTCCGGGACAAGTACGGTGATGGCGTC
  AGCAGGAACCTGGTGGTAGGCACCTTGGCCTGGCCTTCACCTTGGGTCATCGTGGTCGGCTCCTTCTTCT
  CAACATGTGGTGCCGGCCTCCAAAGTCTCACTGGGGCGCCACGTTTACTGCAAGCCATTGCCAAGGATAA
  CATCATCCCCTTCCTCCGGGTGTTTGGCCACGGGAAAGCCAATGGTGAGCCAACGTGGGCCCTCCTCCTG
  ACAGCGCTCATCGCTGAGCTGGGCATCCTCATCGCCTCCCTTGACATGGTGGCCCCCATTCTTTCCATGT
  TCTTTCTGATGTGTTACCTCTTTGTAAACTTGGCCTGTGCTGTGCAGACACTTCTGAGGACCCCCAACTG
  GCGGCCCCGGTTCAAGTACTATCACTGGGCGTTGTCTTTCCTGGGCATGAGTCTGTGCCTGGCTCTCATG
  TTTGTCTCCTCCTGGTACTACGCCCTAGTGGCCATGGTCATCGCAGGCATGATCTACAAGTACATCGAGT
  ACCAAGGGGCTGAGAAGGAGTGGGGTGATGGGATCCGAGGCCTGTCCCTGAGTGCCGCACGATATGCACT
  GCTGAGACTAGAGGAAGGGCCTCCTCACACGAAGAACTGGCGGCCTCAGCTCCTGGTGCTGCTGAAGTTA
  GACGAAGATCTTCATGTGAAGTACCCTCGGCTCCTCACCTTTGCCTCCCAACTTAAGGCTGGGAAAGGCC
  TGACAATCGTTGGCTCTGTCATCCAGGGCAGCTTTCTGGAGAGCTATGGGGAAGCCCAGGCTGCTGAGCA
  GACAATCAAGAACATGATGGAGATTGAGAAAGTAAAAGGCTTCTGCCAGGTAGTGGTGGCCAGCAAGGTT
  CGAGAGGGGCTGGCCCACCTCATCCAGTCTTGCGGCCTGGGTGGCATGAGACATAACTCCGTGGTGCTGG
  GCTGGCCCTATGGCTGGCGACAGAGTGAGGACCCACGTGCCTGGAAGACCTTTATCGACACTGTGCGCTG
  CACCACAGCTGCCCACCTGGCCCTGCTGGTGCCAAAGAACATAGCTTTCTACCCCAGCAACCACGAGCGC
  TACCTGGAGGGCCACATTGATGTGTGGTGGATCGTGCATGACGGAGGCATGCTGATGCTGCTGCCCTTCC
  TGCTGCGCCAGCATAAGGTTTGGAAGAAGTGCCGGATGCGCATTTTCACCGTGGCCCAGATGGACGACAA
  CAGCATCCAGATGAAGAAGGATCTGGCCATCTTCCTGTATCACCTCCGCCTGGAAGCTGAAGTGGAGGTG
  GTAGAGATGCACAACAGTGACATCTCGGCCTACACCTACGAGCGGACACTGATGATGGAGCAGCGGTCTC
  AAATGCTGCGACAGATGAGGCTGACCAAAACAGAGCGGGATCGAGAGGCCCAGCTGGTGAAGGACAGGCA
  CTCGGCTCTGAGGCTAGAGAGCCTCTACTCCGACGAGGAGGATGAGTCTGTGACAGGCGCTGACAAGATC
  CAGATGACATGGACCAGAGACAAGTACATGGCTGAACCCTGGGACCCCAGCCATGCCCCTGACAACTTCC
  GGGAGCTGGTGCACATTAAGCCGGACCAGTCCAATGTGCGGCGTATGCACACTGCTGTGAAGCTCAATGA
  AGTCATTGTCACACGCTCCCATGATGCCCGCCTGGTCCTACTGAACATGCCCGGCCCCCCTAAGAACAGT
  GAGGGTGATGAGAACTACATGGAATTCCTTGAAGTCCTAACCGAGGGCCTTGAACGGGTGTTGTTGGTGC
  GTGGTGGTGGCCGGGAAGTCATCACCATCTATTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201621 representing NM_019229
  Red=Cloning site Green=Tags(s)
  
  MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEREDSDGQGNHRENSPFLSPLDASRGNDYYDRNLALFE
  EELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLR
  LTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTT
  FAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLF
  LACVIISILSIYVGGIKSAFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTA
  DSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSF
  TVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGV
  SRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLL
  TALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALM
  FVSSWYYALVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKL
  DEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKV
  REGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHER
  YLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEV
  VEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEDESVTGADKI
  QMTWTRDKYMAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNS
  EGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019229
• ORF Size: 3255 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019229.1, NM_019229.2, NP_062102.1
• RefSeq Size: 3724
• RefSeq ORF: 3258
• Locus ID: 29501
• MW: 120.6 kDa
• Gene Summary: may act as a potassium-chloride cotransporter [RGD, Feb 2006]