Dhtkd1 (NM_001025720) Rat Tagged ORF Clone

CAT#: RR201719

Dhtkd1 (Myc-DDK-tagged ORF) - Rat dehydrogenase E1 and transketolase domain containing 1 (Dhtkd1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhtkd1
• Synonyms: MGC116097
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201719 representing NM_001025720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCGGCTACTGTGGCAGCTGCAGGGAGAGCTCTCCGCCGAGCGGTCCCGCTTCTCAGGCGCAGTT
  ACCAGACGGAGAGGGGCGTCTATGGATACCGGCCTAGGAAAGCCGGGAGCGGCGAGCCCCGAGGCGACCG
  TGCACGCCCCTCAGTTGACCATGGCCTTGCCAGGCTGGTGACAGTGTATTGTGAGCATGGCCACAAAGCT
  GCCCAGATCAACCCTCTCTTCCCCGGACAAGCCCTCCTGGATACTGTGCCTGAAATCCAAGCTCTTGTGC
  AGACACTCCAGGGACCCTTCACTACCACAGGACTGTTAAACATGGGCAAAGAAGAGGCTTCTCTTGAGGA
  AGTGTTGGCCTATTTGAACCATATCTACTGTGGGCCAATTTCTATTGAAACCGCCCAGCTGCAGAGCCAG
  GAGGAGAAAGACTGGTTTGCCAGAAGGTTTGAGGAACTGAAAAAGGAGACATTTACCACAGAAGAGCGAA
  AACACCTGTCGAAACTACTGCTAGAATCTCAGGAGTTTGACCACTTTTTGGCCACCAAGTTTGCCACAGT
  GAAGAGATACGGAGGTGAAGGGGCAGAGAGCATGATGGGCTTTTTTCACGAGTTGCTGAAATTGTCAGCC
  TATGGCGGTATAACGGACATCATTATCGGGATGCCCCACAGAGGGAGGCTGAATCTGTTGACAGGCCTTC
  TGCAGTTACCTCCAGAGCTGATGTTCCGTAAAATGCGAGGCTTAAGTGAGTTTCCAGAAAACGTTGCTGC
  CATTGGTGACGTCCTGTCCCATCTGACCTCCTCAGTGGACCTAGACTTTGGGGCCCATCGACCCCTCCAT
  GTGACAATGCTACCCAACCCCTCACACCTGGAAGCTATTAACCCCGTGGCTGTAGGGAAAACTCGTGGCA
  GGCAGCAGTCTCAGGAAGACGGGGACTACTCACCAAATGGTTCTGCTCAGCCTGGGGACAAAGTCATATG
  CCTGCAGGTTCATGGCGACGCTTCCTTCTGCGGTCAAGGGATTGTTCTGGAGACGTTCACGCTCTCTAAT
  CTCCCACATTTCAGAATCGGTGGGAGCATCCATCTCATAGTCAATAACCAGTTGGGTTACACCACGCCTG
  CTGAGAGAGGAAGGTCGTCCTTATACAGCAGTGACATTGGAAAGCTAGTAGGCTGTGCCATCATCCATGT
  CAACGGAGACAGTCCAGAGGAAGTGGTCCGAGCGACACGACTTGCTTTTGAGTATCAGCGCCAGTTCCGC
  AAGGACGTGATTATTGATTTGTTATGCTATAGGCAGTGGGGTCACAATGAGCTGGACGAACCTTTCTTCA
  CCAACCCGGTCATGTACAAAATCATCAGAGCTCGAAAGAGCATTCCTGATACCTACGCAGAGCACCTCAT
  CGCCAGCGGACTCATGACGCAGGAGGAGGTCTCCGACATAAAAGCCTCCTACTACGCCAAGTTAAATGGC
  CACTTGGCCAATGTGGCCCACTACAGCCCCCCGGCCCCGCACCTGCAGGCCCGCTGGCAGGGCCTGGTGC
  AGCCAGCAGCCTGCGTCACCACCTGGGACACGGGCGTGCCGCTGGAGCTGCTACGGTTCGTTGGCGTGAA
  GTCCGTGGAGGTTCCAGAGGAGCTGCAGTTACACAGCCACCTGCTAAAGATGTATGTTCAGTCAAGAATG
  GAGAAGGTGAAGAATGGAACCAATCTTGACTGGGCCACCGCAGAAACACTTGCCTTGGGCTCCTTACTTG
  CTCAAGGTTTCAATGTTCGTCTGAGTGGCCAAGATGTTGGCCGTGGAACTTTCAGTCAAAGGCATGCAAT
  GGTGGTTTGCCAGAACACTGATGATGTCTACATTCCTCTGAACCACATGGACCCTAACCAGAAGGGCTTT
  CTGGAGGTTAGCAACAGCCCGCTGTCAGAAGAGGCTGTCTTGGGATTTGAATACGGGATGAGCATCGAAA
  GCCCAAAGTTGCTGCCCCTCTGGGAAGCTCAGTTTGGCGATTTCTTCAATGGAGCCCAGATCATCTTTGA
  CACATTCATCTCTGGAGGCGAGGCCAAGTGGCTCCTGCAGAGTGGCCTTGTCATCCTCCTTCCACACGGC
  TATGATGGAGCCGGGCCTGACCACTCATCCTGTCGGATAGAGCGCTTCTTGCAGATGTGCGACAGTGCGG
  AAGAAGGCGTGGACAGCGACACTGTGAACATGTTTGTGGTTCACCCGACTACGCCTGCTCAGTACTTCCA
  CTTGCTTAGAAGACAGATGATGCGGAACTTCAGAAAGCCTCTCATCGTTGCATCTCCCAAAATGTTACTC
  AGGTACCCTGTAGCTGTGTCAACGCTTGAGGAGATGGCGCCAGGAACAGCATTCAAACCTGTCATTGGTG
  ATTCGTCGGTAGACCCCAAAAATGTCAAAACCCTCATATTCTGCTCCGGCAAACATTTTTATGCCCTGCT
  GAAGCAAAGAGAGTCTCTGGGGGCTAAGAAGCGTGATTTTGCCATCATTCGACTAGAGGAACTCTGCCCT
  TTCCCGCTGGATTCATTACAACAAGAGATGGGCAAATACAAACACGTTCAAGATATCATCTGGAGTCAGG
  AGGAGCCTCAGAACATGGGTCCGTGGTCTTTTGTTTATCCAAGGTTTGAAAAGCAACTTGCTTGCAAGCT
  CCGTCTCGTAAGTCGACCCCCTTTGCCAGCGCCCGCGGTGGGAATTGGCACAGTGCACCAGCAGCAGCAT
  GAAGCCATCCTCTTCAAGACCTTCACTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201719 representing NM_001025720
  Red=Cloning site Green=Tags(s)
  
  MASATVAAAGRALRRAVPLLRRSYQTERGVYGYRPRKAGSGEPRGDRARPSVDHGLARLVTVYCEHGHKA
  AQINPLFPGQALLDTVPEIQALVQTLQGPFTTTGLLNMGKEEASLEEVLAYLNHIYCGPISIETAQLQSQ
  EEKDWFARRFEELKKETFTTEERKHLSKLLLESQEFDHFLATKFATVKRYGGEGAESMMGFFHELLKLSA
  YGGITDIIIGMPHRGRLNLLTGLLQLPPELMFRKMRGLSEFPENVAAIGDVLSHLTSSVDLDFGAHRPLH
  VTMLPNPSHLEAINPVAVGKTRGRQQSQEDGDYSPNGSAQPGDKVICLQVHGDASFCGQGIVLETFTLSN
  LPHFRIGGSIHLIVNNQLGYTTPAERGRSSLYSSDIGKLVGCAIIHVNGDSPEEVVRATRLAFEYQRQFR
  KDVIIDLLCYRQWGHNELDEPFFTNPVMYKIIRARKSIPDTYAEHLIASGLMTQEEVSDIKASYYAKLNG
  HLANVAHYSPPAPHLQARWQGLVQPAACVTTWDTGVPLELLRFVGVKSVEVPEELQLHSHLLKMYVQSRM
  EKVKNGTNLDWATAETLALGSLLAQGFNVRLSGQDVGRGTFSQRHAMVVCQNTDDVYIPLNHMDPNQKGF
  LEVSNSPLSEEAVLGFEYGMSIESPKLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGLVILLPHG
  YDGAGPDHSSCRIERFLQMCDSAEEGVDSDTVNMFVVHPTTPAQYFHLLRRQMMRNFRKPLIVASPKMLL
  RYPVAVSTLEEMAPGTAFKPVIGDSSVDPKNVKTLIFCSGKHFYALLKQRESLGAKKRDFAIIRLEELCP
  FPLDSLQQEMGKYKHVQDIIWSQEEPQNMGPWSFVYPRFEKQLACKLRLVSRPPLPAPAVGIGTVHQQQH
  EAILFKTFTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025720
• ORF Size: 2760 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025720.1, NP_001020891.1
• RefSeq Size: 4280 bp
• RefSeq ORF: 2763 bp
• Locus ID: 361272
• Cytogenetics: 17q12.3
• MW: 102.6 kDa