Tada2a (NM_001012141) Rat Tagged ORF Clone

CAT#: RR201809

Tada2a (Myc-DDK-tagged ORF) - Rat transcriptional adaptor 2 (ADA2 homolog, yeast)-like (Tada2l), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tada2a
• Synonyms: Tada2l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201809 representing NM_001012141
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGTTTGGGTTCCTTTAGCAATGATCCATCTGATAAGCCACCTTGCAGAGGCTGCTCCTCCTACC
  TCATGGAACCTTACATTAAATGTGCTGAATGTGGGCCTCCTCCTTTCTTCCTGTGCTTGCAGTGTTTCAC
  TAGAGGATTTGAGTACAAGAAACATCAGAGTGATCATACTTACGAAATAATGACCTCAGACTTCCCTGTT
  CTTGATCCCAGCTGGACAGCTCAGGAAGAAATGGCACTTTTAGAAGCTGTTATGGACTGTGGCTTTGGAA
  ATTGGCAGGATGTTGCTAATCAGATGTGCACCAAAACCAAGGAAGAGTGTGAGAAGCACTACATGAAACA
  TTTTATCAATAACCCTCTGTTTGCATCCACCCTGCTAAACCTGAAGCAAGCTGAGGCAGCCAAAACTGCC
  GACACAGCCATTCCATTTCACTCTGCAGATGACCCTCCCCGGCCTGCCTTTGATTCGTTGCTGTCTCGGG
  ACATGGCAGGATATATGCCAGCTCGGGCAGATTTCATTGAGGAGTTTGACAATTATGCAGAATGGGACTT
  AAGAGACATTGATTTTGTTGAAGACGACTCGGACATCTTACACGCTCTGAAGATGGCTGTGGTGGATATC
  TATCATTCCAGGTTAAAAGAGAGGCAAAGACGAAAAAAGATTATAAGAGATCACGGATTAGTTAACCTTA
  GAAAGTTCCGACTGATGGAGCGTCGGTATCCTAAGGAGGTCCAAGACCTTTATGAAACGATGAGGAGATT
  TGCAAGGATTGTGGGACCAGTGGAGCACGACAAGTTCATTGAAAGCCATGCATTGGAATTTGAACTCCGA
  AGGGAAATCAAGAGGCTCCAAGAATATAGGACAGCAGGCATTACCAATTTTTGTAGCGCCAGGACTTATG
  ACCACCTGAAGAAGACACGAGAGGAGGAGCGCCTTAAACGCACCATGCTCTCAGAAGTTCTCCAGTACAT
  CCAGGACAGCAGTGCGTGCCAGCAGTGGCTGCGCCGGCAGGCTGACATTGATTCTGGGCTGAGTCCTTCT
  GTTCTGATGGCTTCCAACTCAGGAAGACGCAGTGCACCACCCTTGAACCTCACGGGTCTCCCTGGAACAG
  AGAAACTCAATGAAAAAGAAAAGGAGCTCTGTCAGGTGGTAAGGTTGGTACCTGGAGCCTATTTGGAATA
  TAAGTCTGCTCTATTGAATGAGTGTCACAAGCAAGGAGGCTTGAGACTGGCACAAGCAAGAGCACTCATC
  AAGATAGACGTGAACAAAACCCGGAAGATCTATGACTTCCTCATCCGAGAAGGGTACATCACGAAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201809 representing NM_001012141
  Red=Cloning site Green=Tags(s)
  
  MDRLGSFSNDPSDKPPCRGCSSYLMEPYIKCAECGPPPFFLCLQCFTRGFEYKKHQSDHTYEIMTSDFPV
  LDPSWTAQEEMALLEAVMDCGFGNWQDVANQMCTKTKEECEKHYMKHFINNPLFASTLLNLKQAEAAKTA
  DTAIPFHSADDPPRPAFDSLLSRDMAGYMPARADFIEEFDNYAEWDLRDIDFVEDDSDILHALKMAVVDI
  YHSRLKERQRRKKIIRDHGLVNLRKFRLMERRYPKEVQDLYETMRRFARIVGPVEHDKFIESHALEFELR
  REIKRLQEYRTAGITNFCSARTYDHLKKTREEERLKRTMLSEVLQYIQDSSACQQWLRRQADIDSGLSPS
  VLMASNSGRRSAPPLNLTGLPGTEKLNEKEKELCQVVRLVPGAYLEYKSALLNECHKQGGLRLAQARALI
  KIDVNKTRKIYDFLIREGYITKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012141
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012141.1, NP_001012141.1
• RefSeq Size: 2124 bp
• RefSeq ORF: 1332 bp
• Locus ID: 360581
• Cytogenetics: 10q26
• MW: 51.4 kDa