Ccdc8 (NM_001009533) Rat Tagged ORF Clone

CAT#: RR201983

Ccdc8 (Myc-DDK-tagged ORF) - Rat similar to coiled-coil domain containing 8 (MGC72567), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201983 representing NM_001009533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGATTGGGGAGGATGTTGACTATCTGCTCATCCCCCGGGAGGTCCGGCTGGCTGGGGGCGTGT
  GGAGAGTCATCTCCAAGCCTGCCACTAAGGAGGCTGTGTTTCGGGAGCGGCTGATCCAGTTTCTGCAGGA
  GGAGGGCCGCACCCTGGAAGATGTGGCTCGTATCATCGAAAAGAGTACTCCACATCCACCCCAGCCCCCC
  AAAAAAGCCAAGGTGCCCAGAGTGAGGAGAGTCCCGCAGATGGTCACTCCTCCACTTCGGCTGGTTGTAG
  GCACTTACGACAGCAGCAATGGCAGCGATAGCGAGTTGAGTGACTTCGATACATCCAAAGTCAAAGGCAA
  CAGGGGCTCCGGGAAGACCAGGAAGGTGCGCAAAATGCCGGTCAGTTACTTGGGCAGCAAATTCCTGGGC
  AGCGATGAGAGCGAGGATGATCAGGAGCTGGTAGAGGCCTTCCTCCGCCGCGGGGAGAAGAAGCCCAGCG
  CGCCTCCTCCACGGCGCCGAGTCAATCTTCCAGTGCCCATGTTTGAGAATAACCTGGGGCCCCAGCCATC
  CAAGGGGGACAGATGGCGGGAGTATGTCAGCCAGGTGTCCTGGGGGAAGCTGAAACAGAGGGTGAAAGGC
  TGGGCGCCCAGATCAGGCTCCGAGGTGGGCCAGGCCCAGCAGTCCTCCATAGCTGAGAGGGCTGGAGAAA
  TGAGACACAGCCACACCAGCCCAGACCTGGATGATAGCTCCAGAAACACAGGAGACCTGAGCGACCAGAC
  ACTGATTACTAGACGCTGGAAGCCCAAGATCAAGTGGGTCTCCTTGAGACGATGCAGGAAGGAGCAGGTG
  CCACCCTCGGCTCACGGGACAGCTGAAGAGCCCCCAGAGGCTGCAGAAAACCAGGGAGCAGGGGCTGCAG
  CTGAACATGGGGTAGAGGCTGCAGCTAGCCAGAGGCCAGAGGCTGCAGCTAGTCCAAGAGCAGAGGCTGC
  AGCTAATCCAAGAGCAGAGGCTACAGCTAATCCAAGAGCAGAGGCTGCAGCTAATCCAAGAGCAGAGGCT
  GCAGCTAGTCCAAGAGCAGAGGCTGCAGCTAATCCAAGAGCAGAGGCTGCAGCTAATCCAAGAGCAGAGG
  CTACAGCTAATCCAAGAGCAGAGGCTGCAGCTAATCCAAGAGCAGAGGCTACAGCTAATCCAAGAGCAGA
  GGCTGCAGTTAATCCAAGAACAGAGGCTGCAGCTAATCCAAGAGCAGAGGCTGCAGTTAATCCAAGAGCA
  GAGGCTACAGCTAGTCCAGGGGCAGAGGCTGCAGCTAGTCCAAAGGCAGAGGCTGCAGCTAGTCCAAGGG
  CAGAGGCTGAAGTTAACCAGAAGACAGAGGCTACAGCTAGTCCAAGGGCAGAGACTGCAGCTAGTCCAAG
  GGTAGAGGCTGCAGCTAGTCTAAGGGTAGAGGCTGCAGCTAGTCCAAGGGCAGAGGCTACAGTTAGTCCA
  AGGGCAGAGGCTGTAGCTACTCCAAGAGCAGAGACTGCTGCTAGTGCAAGGATAGAAGCTGCAGCTAATC
  TCAGGGCAGGAGTTCTACCTGACCAGAGGGCAGAAGCCATAGATAGTCAGAGGGCAGAGGGCCCAGTTAA
  CCAAAGCACTGGTGCTACAGAAAATCAGAGGGTGGAGGTCCTAGCTGACCAGAGGGCAGGGGTCCTCCAT
  GACCAGAGGGAGGAGGCTGGACCTCAAGCTATACTGGAAGCCTCAGCTGATTCGGGATCTAGAGCCCGGA
  AACAGGTAAAGACAGTGAGGTTCCAGACCCCAGGACGCTTCTCCTGGTTTCACATGCGCAGGAAAGCTTT
  CTGGCACACTCCCAGGTTGCCAACCCTGCCCAAGAGAGGCCCCAGGGCAGGGGCGGGAGAGGCTAGGAGC
  CTCAGGGTGCTAAGGGCTGACACCAGAGCAGACATGGAACACAGAGAGCAAGAAGAACAGCTGCTCAGGG
  TGCTAAGGGCTGACACCAGAGCAGACATGGAACACAGAGAGCAAGAAGAACAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201983 representing NM_001009533
  Red=Cloning site Green=Tags(s)
  
  MLQIGEDVDYLLIPREVRLAGGVWRVISKPATKEAVFRERLIQFLQEEGRTLEDVARIIEKSTPHPPQPP
  KKAKVPRVRRVPQMVTPPLRLVVGTYDSSNGSDSELSDFDTSKVKGNRGSGKTRKVRKMPVSYLGSKFLG
  SDESEDDQELVEAFLRRGEKKPSAPPPRRRVNLPVPMFENNLGPQPSKGDRWREYVSQVSWGKLKQRVKG
  WAPRSGSEVGQAQQSSIAERAGEMRHSHTSPDLDDSSRNTGDLSDQTLITRRWKPKIKWVSLRRCRKEQV
  PPSAHGTAEEPPEAAENQGAGAAAEHGVEAAASQRPEAAASPRAEAAANPRAEATANPRAEAAANPRAEA
  AASPRAEAAANPRAEAAANPRAEATANPRAEAAANPRAEATANPRAEAAVNPRTEAAANPRAEAAVNPRA
  EATASPGAEAAASPKAEAAASPRAEAEVNQKTEATASPRAETAASPRVEAAASLRVEAAASPRAEATVSP
  RAEAVATPRAETAASARIEAAANLRAGVLPDQRAEAIDSQRAEGPVNQSTGATENQRVEVLADQRAGVLH
  DQREEAGPQAILEASADSGSRARKQVKTVRFQTPGRFSWFHMRRKAFWHTPRLPTLPKRGPRAGAGEARS
  LRVLRADTRADMEHREQEEQLLRVLRADTRADMEHREQEEQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009533
• ORF Size: 1953 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3408 bp
• RefSeq ORF: 1956 bp
• Locus ID: 494320
• Cytogenetics: 1q21
• MW: 70.1 kDa