Ppp2r1b (NM_001025418) Rat Tagged ORF Clone

CAT#: RR202007

Ppp2r1b (Myc-DDK-tagged ORF) - Rat protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform (Ppp2r1b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp2r1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202007 representing NM_001025418
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCAGCAGGGCCAGGGACCGTTCCGGGGGCAGCGGGCGGAGATGGAGACGATTCACTCTACC
  CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAGGACGTGCAGCTCCGTCTCAACAGTATTAAGAAGTT
  ATCAACTATTGCTCTAGCACTCGGGGTAGAAAGGACTCGAACTGAACTGCTGCCTTTCCTTACAGATACA
  ATTTATGATGAAGATGAGGTGCTGTTAGCTCTTGCCGAGCAGCTGGGGAATTTCACTGGTTTGGTGGGAG
  GCCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAGTTTGGCTACGGTGGAGGAGACTGTCGTTCG
  AGACAAGGCTGTGGAGTCCCTGAGGCAGATCTCCCAGGAGCACACTCCCGTTGCCCTGGAAGCTCATTTT
  GTACCCCTGGTGAAGCGCTTAGCAAGTGGGGATTGGTTCACATCTCGCACATCTGCATGCGGTTTGTTCA
  GCGTTTGCTACCCCAGAGCTTCAAATGCTGTCAAGGCAGAGATCCGACAGCACTTCCGTTCCCTGTGCTC
  GGATGATACACCAATGGTGCGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA
  GACAGTGTTAAAACTGAAATTGTTCCATTATTCACGAATCTGGCTTCAGATGAACAGGATTCTGTGCGCC
  TGCTAGCTGTAGAAGCGTGTGTCAGTATTGCCCAGCTACTGTCTCAGGATGACCTGGAAGCCTTGGTAAT
  GCCTACACTTCGGCAAGCAGCAGAAGATAAATCTTGGCGGGTTCGCTACATGGTGGCTGACAAATTTTCA
  GAGCTCCAGAAAGCGGTGGGCCCTAAAATTGCACTCAGTGACCTCATCCCCGCCTTCCAGAGCCTCCTCA
  GAGACTGCGAGGCCGAAGTCCGAGCAGCCGCTGCCCACAAAGTAAGAGAGCTTTGTGAGAACCTGCCCAC
  TGAAGGCAGAGAGACCGTGATTATGAACCAAATCCTGCCCTATATAAAGGAATTAGTGTCTGATACCAAC
  CAACATGTCAAGTCAGCCCTAGCTTCTGTAATTATGGGATTATCTACAGTTTTGGGCAAAGAGAATACCA
  TTGAACATCTTCTGCCTCTATTTTTAGCTCAATTAAAGGATGAGTGTCCAGAAGTTCGTTTAAATATCAT
  CTCCAATTTGGATTGTGTGAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTCCCTGCCATT
  GTGGAACTGGCTGAGGACGCCAAGTGGAGGGTCCGCTTGGCGATCATTGAGTACATGCCGCTGCTGGCTG
  GCCAGCTGGGTGTGGAGTTTTTTGATGAAAAGCTGAATTCTTTGTGTATGGCCTGGCTTGTGGATCATGT
  CTATGCCATTCGGGAAGCAGCAACCAACAACCTCATGAAGTTAGTCCAGAAGTTTGGTACCGAGTGGGCC
  CAGAACACCATCGTCCCCAAAGTGTTGGTAATGGCAAATGATCCGAACTACTTGCACAGGATGACCACCT
  TATTCTGCATTAATGCCCTCTCTGAAGCCTGTGGTAAGGAAATAACCACTAAGCAAATGTTGCCTATCGT
  GTTGAAAATGGCTGGAGACCAGGTGGCAAATGTCCGCTTCAATGTGGCTAAGTCACTGCAGAAGATTGGA
  CCCATTCTAGACACCAACGCCTTGCAGGGGGAAGTGAAGCCGGTGCTGCAGAAATTAGGTCAGGATGAAG
  ACATGGATGTCAAATATTTTGCACAGGAAGCTATAAGTGTTCTTGCCTTGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202007 representing NM_001025418
  Red=Cloning site Green=Tags(s)
  
  MAGAAGPGTVPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRTELLPFLTDT
  IYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLESLATVEETVVRDKAVESLRQISQEHTPVALEAHF
  VPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQHFRSLCSDDTPMVRRAAASKLGEFAKVLEL
  DSVKTEIVPLFTNLASDEQDSVRLLAVEACVSIAQLLSQDDLEALVMPTLRQAAEDKSWRVRYMVADKFS
  ELQKAVGPKIALSDLIPAFQSLLRDCEAEVRAAAAHKVRELCENLPTEGRETVIMNQILPYIKELVSDTN
  QHVKSALASVIMGLSTVLGKENTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAI
  VELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWA
  QNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGKEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIG
  PILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVLALA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025418
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025418.1, NP_001020589.1
• RefSeq Size: 3207 bp
• RefSeq ORF: 1806 bp
• Locus ID: 315648
• Cytogenetics: 8q23
• MW: 66 kDa