Actn4 (NM_031675) Rat Tagged ORF Clone

CAT#: RR202011

Actn4 (Myc-DDK-tagged ORF) - Rat actinin alpha 4 (Actn4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actn4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202011 representing NM_031675
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACTATCACGCGGCGAACCAGGCGTACCAGTACGGCCCAAGCAGCGGCGGCAATGGCACGGGCG
  GCGGCGGAGGCATGGGCGACTACATGGCCCAGGAGGATGACTGGGACCGGGACCTGCTGTTGGACCCGGC
  CTGGGAGAAGCAGCAGCGCAAGACCTTCACAGCCTGGTGCAACTCCCATCTGCGGAAGGCTGGCACTCAG
  ATCGAGAACATCGATGAGGATTTCCGGGATGGGCTCAAACTTATGCTTCTGCTGGAGGTCATTTCAGGGG
  AGCGGTTGCCTAAGCCAGAGCGGGGGAAGATGCGAGTGCACAAGATCAACAATGTAAACAAAGCCCTGGA
  CTTCATCGCCAGCAAGGGAGTCAAGCTGGTGTCCATTGGGGCAGAAGAGATCGTGGATGGCAATGCAAAG
  ATGACCCTGGGAATGATCTGGACCATCATCCTCAGGTTCGCCATCCAAGACATCTCTGTGGAAGAGACCT
  CTGCCAAGGAAGGACTCCTCCTCTGGTGCCAGAGAAAGACAGCCCCGTATAAAAACGTCAACGTCCAGAA
  CTTCCATATCAGCTGGAAGGATGGTCTGGCCTTCAACGCGCTCATCCACCGGCACAGACCTGAGCTGATT
  GAGTATGACAAACTGAGGAAGGATGATCCAGTCACCAACCTAAACAATGCATTTGAAGTGGCTGAGAAAT
  ACCTTGATATCCCCAAGATGTTGGATGCTGAGGACATCGTGAACACAGCCCGGCCCGACGAGAAGGCCAT
  AATGACATATGTGTCCAGCTTCTACCATGCCTTTTCAGGAGCGCAGAAGGCTGAGACTGCTGCCAACCGG
  ATCTGCAAGGTGCTGGCTGTGAACCAGGAAAATGAGCACCTGATGGAAGATTACGAGCGCCTGGCCAGTG
  ATCTTCTAGAGTGGATCCGGCGCACCATCCCCTGGCTGGAAGACCGTGTGCCTCAGAAGACCATCCAGGA
  GATGCAGCAGAAGCTGGAGGACTTCCGAGACTATAGGCGTGTGCACAAGCCCCCCAAGGTACAGGAGAAG
  TGTCAGCTGGAGATCAACTTCAACACCCTGCAGACCAAGCTGCGGCTGAGCAACCGGCCTGCCTTCATGC
  CCTCCGAGGGCAGGATGGTCTCGGACATCAACAATGGGTGGCAACATCTGGAGCAGGCTGAGAAGGGCTA
  TGAAGAATGGCTCTTGAATGAAATCCGTAGGCTGGAACGACTTGATCACCTGGCAGAGAAATTCCGGCAG
  AAGGCTTCCATCCATGAGGCCTGGACCGATGGGAAGGAGGCCATGCTGAAGCACCGGGACTATGAGACAG
  CCACCCTGTCAGACATCAAAGCTCTGATCCGAAAGCATGAGGCCTTTGAGAGTGACCTGGCGGCGCACCA
  GGACCGCGTGGAGCAGATCGCGGCTATTGCCCAGGAGCTCAACGAACTGGACTACTATGACTCCCACAAC
  GTCAACACACGGTGCCAGAAGATCTGTGACCAGTGGGATAACCTGGGCTCTCTGACCCACAGTCGCAGGG
  AAGCCTTGGAGAAAACAGAGAAACAGCTGGAGACCATCGACCAGCTACACTTGGAGTATGCTAAGCGGGC
  CGCGCCCTTCAACAACTGGATGGAGAGTGCCATGGAGGACCTGCAGGACATGTTCATCGTCCACACCATC
  GAGGAGATTGAGGGCCTGATCTCAGCCCACGACCAGTTCAAGTCCACCCTGCCAGATGCCGACAGGGAGC
  GTGAGGCCATCCTGGCCATCCACAAAGAGGCCCAAAGGATCGCTGAGAGCAATCACATCAAGCTGTCGGG
  CAGCAACCCCTACACCAGCGTTACACCCCAGATCATCAACTCCAAATGGGAGAAGGTGCAGCAGCTGGTG
  CCGAAGCGGGACCACGCACTCCTGGAAGAGCAGAGCAAGCAGCAATCCAATGAGCACCTTCGCCGACAGT
  TTGCCAGCCAAGCCAATATGGTGGGGCCCTGGATCCAGACCAAGATGGAGGAGATTGGGCGCATCTCCAT
  CGAGATGAACGGGACTCTGGAAGACCAGCTGAGTCACTTGAAGCAGTACGAGCGCAGCATTGTGGACTAC
  AAGCCCAACCTGGACTTGCTGGAGCAGCAGCACCAGCTCATCCAGGAGGCCCTCATCTTCGACAACAAGC
  ACACCAACTACACAATGGAGCACCTCCGTGTGGGCTGGGAGCAGCTGCTCACCACCATTGCCCGCACCAT
  CAACGAGGTAGAGAATCAGATCCTCACCCGAGATGCCAAGGGCATCAGCCAGGAGCAAATGCAGGAGTTC
  CGAGCCTCCTTCAACCACTTTGACAAGGACCATGGCGGGGCACTGGGGCCTGAGGAATTCAAGGCCTGCC
  TCATCAGCCTGGGCTACGACGTGGAGAATGACCGGCAGGGTGATGCTGAGTTCAACCGGATCATGAGTGT
  GGTCGACCCCAACCATAGTGGCCTCGTGACCTTCCAAGCCTTCATTGACTTCATGTCAAGGGAGACCACA
  GACACAGACACCGCCGATCAGGTCATCGCCTCCTTCAAGGTCCTGGCCGGGGACAAGAACTTCATCACTG
  CTGAGGAACTTCGGAGAGAGCTGCCCCCTGACCAGGCTGAGTACTGCATCGCCCGCATGGCGCCCTACCA
  GGGCCCTGATGCTGCTCCAGGCGCCCTTGACTACAAGTCCTTCTCTACAGCCCTCTATGGGGAGAGCGAC
  CTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202011 representing NM_031675
  Red=Cloning site Green=Tags(s)
  
  MVDYHAANQAYQYGPSSGGNGTGGGGGMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQ
  IENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAK
  MTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELI
  EYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANR
  ICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEK
  CQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQ
  KASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHN
  VNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTI
  EEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLV
  PKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDY
  KPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEF
  RASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETT
  DTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESD
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031675
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031675.2, NP_113863.2
• RefSeq Size: 3885
• RefSeq ORF: 2736
• Locus ID: 63836
• MW: 104.9 kDa
• Gene Summary: alpha-actinin cytoskeletal protein that anchors various actin-containing microfilaments to cellular structures; anchors V myosins to cellular structures via the RING finger-containing protein BERP [RGD, Feb 2006]