Rps6kb2 (NM_001010962) Rat Tagged ORF Clone

CAT#: RR202057

Rps6kb2 (Myc-DDK-tagged ORF) - Rat ribosomal protein S6 kinase, polypeptide 2 (Rps6kb2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rps6kb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202057 representing NM_001010962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGTGTTTGATTTAGACTTGGAGACCGAGGAAGGAAGTGAGGGCGAGGGCGAGCCGGAGTTCA
  ACCCTGCGGACGTGTGTCCCCTTGGCGAGTTAAGGGCTGCTGGCCTGGAGACAGTGGGACACTATGAGGA
  GGTGGAACTGACAGAGAGCAGCGTCAACCTGGGTCCTGAGCGCATCGGACCCCACTGCTTTGAGCTACTG
  AGTGTTCTGGGCAAGGGGGGTTATGGCAAGGTGTTCCAGGTGAGAAAAGTGCAAGGCACCAACTTGGGCA
  AAATATATGCCATGAAAGTCTTGAGAAAGGCCAAGATTGTATGCAGTGCCAAGGACACAGCACATACCCG
  GGCTGAGCGGAACATTCTAGAATCGGTGAAGCACCCCTTCATTGTAGAACTGGCCTATGCTTTCCAGACA
  GGTGGCAAACTCTACCTCATCCTGGAGTGCCTCAGTGGTGGTGAGCTCTTCACACATCTTGAGCGAGAAG
  GCATCTTCCTGGAAGACACAGCCTGCTTCTACCTGGCAGAGATCACACTAGCCCTGGGCCATCTCCATTC
  CCAAGGCATCATCTACCGGGATCTCAAGCCCGAGAACATCATGCTCAACAGCCAGGGCCATATCAAACTG
  ACAGACTTTGGACTCTGCAAAGAGTCCATTCATGAGGGTGCTATCACTCACACCTTCTGTGGCACCATTG
  AGTACATGGCCCCAGAGATTCTAGTGCGCATCGGTCACAACCGGGCAGTGGACTGGTGGAGCCTGGGAGC
  CCTGATGTACGACATGCTCACTGGATCGCCGCCCTTCACCGCAGAGAACCGGAAGAAAACTATGGATAAA
  ATCATTAAAGGGAAGCTGGTGCTGCCCCCCTACCTCACCCCGGATGCCCGGGACCTTGCCAAAAAGTTTC
  TGAAGCGGAATCCCACTCATCGAATTGGGGGTGGCCCAGGAGATGCTGCTGATGTGCAGAGGCACCCGTT
  TTTCCGGCACATCAATTGGGATGACCTCTTGGCCCATCGTGTAGACCCTCCCTTCAGACCGAGTCTGCAA
  TCAGAAGAGGACGTGAGCCAGTTTGATGTGCGATTCACACGGCAGACGCCAGTAGATAGTCCAGATGACA
  CAGCCCTCAGTGAGAGTGCCAACCAAGCCTTCCTGGGCTTCACGTATGTGGCACCTTCTGTACTGGACAG
  CATCAAAGAGGGCTTCTCCTTCCAGCCTAAGCTGCGCTCCCCCAGACGCCTTAACAGCAGTCCCCGCACC
  CCTATCAGCCCCCTCAAGTTCTCTCCCTTTGAGGGGTTCCGGCCCAGTCCTGGCCCACCAGAGCCCATGG
  AGCCATCTCTACCTCCACTCCTGCCACCACCATCATCACCACCACCCACAAGTACTGCCCCACTTCCCAT
  CCGTCCCCCCTCAGGAACCAAGAAGTCCAAGAAGGGACGGGGCCGCCCTGGGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202057 representing NM_001010962
  Red=Cloning site Green=Tags(s)
  
  MAAVFDLDLETEEGSEGEGEPEFNPADVCPLGELRAAGLETVGHYEEVELTESSVNLGPERIGPHCFELL
  SVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVCSAKDTAHTRAERNILESVKHPFIVELAYAFQT
  GGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSQGIIYRDLKPENIMLNSQGHIKL
  TDFGLCKESIHEGAITHTFCGTIEYMAPEILVRIGHNRAVDWWSLGALMYDMLTGSPPFTAENRKKTMDK
  IIKGKLVLPPYLTPDARDLAKKFLKRNPTHRIGGGPGDAADVQRHPFFRHINWDDLLAHRVDPPFRPSLQ
  SEEDVSQFDVRFTRQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSIKEGFSFQPKLRSPRRLNSSPRT
  PISPLKFSPFEGFRPSPGPPEPMEPSLPPLLPPPSSPPPTSTAPLPIRPPSGTKKSKKGRGRPGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010962
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010962.2, NP_001010962.2
• RefSeq Size: 1769 bp
• RefSeq ORF: 1458 bp
• Locus ID: 361696
• Cytogenetics: 1q43
• MW: 53.6 kDa