Phlpp1 (NM_021657) Rat Tagged ORF Clone

CAT#: RR202102

Phlpp1 (Myc-DDK-tagged ORF) - Rat PH domain and leucine rich repeat protein phosphatase (Phlpp), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phlpp1
• Synonyms: Phlpp; Plekhe1; Scop
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202102 representing NM_021657
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCCGCCGCAGCCCCGGCACAGCGACTCGCGGACCCCACGGGTGAGGACCGAGCCCCGGCGG
  CGGCGGCGGCGGCCGAGGGCGGACGGAGTCCGGATTCTGTGCTGAGCGCGGCGGCCCCGAGCGGCGGGAA
  CGGCGGCGCGGCGCGAGAGGAGGCCCCCTGCGAGGCGCCGCCGGGGCCGCTGCCCGGCCGCGCGGGAGGC
  ACCGGACGCAGGCGTCGGCGCGGGGTGCCCCAGCCCGCGGCCGGCGGGGCCGCCCCGGTGACCGCGGCCG
  GCGGCGGCGCCAACTCGCTGCTGCTGAGGAGAGGGCGGCTGAAGAGGAATCTGTCGGCCGCCGCTTCGTC
  GTCCTCATCGCCGTCCTCGGCGTCTTCGGCCGCTGGCGGCCTCCCGGCGTCCTGCTCGGCCTCGGCGTCG
  CTGTGCACCCGGAGTCTGGACAGGAAGACGCTGCTCCAGAAGCACCGGCAGCTGCTTCAGCTGCAGCCGT
  CGGACCGGGACTGGGTGAGGCACCAGCTCCAGCGGGGCTGTGTGCACGTCTTTGACCGCCACATGGCCTC
  GTCCTACCTGCGCCCAGTGCTCTGCACGCTGGACACCACAGCCGCCGAGGTGGCCGCCCGGCTTCTGCAG
  CTGGGCCACAAAGGCGGCGGGGTGGTGAAGGTGCTGGGCCACGGGCCACCTCCGGCCGCTGCCCCGGCCG
  CCAGCGATCAGACCCCGGCCACAGAGCTCGGAAGAGACGTGGAGCCACCGCCCTCGAGCAGCACGGTCGG
  TGCTGTCCGGGGCCCTGCGCGCGCGCCTCCTGCCGATCTGCCGCTGCCCGGGGGCGCCTGGACGCGCTGT
  GCACCTCGGGTCAACCCTGCGCCCTCGGACTCCAGCCCCGGAGAGCTGTTCGCGGGCGGCCCCTGTTCCC
  CGTCCCGCGCCCCGCGCCCGGCCTCCGACACCGAGAGCTTCAGCCTGAGCCCCAGCGCCGAGAGCGTGTC
  AGACCGGCTGGACCCCTATAGCAGCGGCGGCGGCTCCTCCTCTTCGTCCGAGGAACTAGAAGCTGACCCG
  GCCACGGTCTTGACGGGGCCTTCCGGGCCGCCCCACCACCCAGTCCGCTCCTCACAGCCTCGACCGCCTT
  CCCCGAAGACCTCGGCGTTACTGCAGCCGAAAGCCCCGACGGGCGTAGACGGCACTGGCCTAGTGGTGGG
  CGAAGGTCCCGGAGACGACAAGGCGGTGGCAGCTGCAGCTCCAGGCGTTCCTCTGTGGACTCCAGGGAGG
  ATCCGGGAGACCGTGCAAAAGACGTCTTCTCCGCCCTCCCTGTATGTACAGCTCCATGGGGAGACTACCC
  GGCGCCTGGAGGCGGACGAGAAGCCATTGCAGATCCAAAATGACTACCTCTTCCAACTGGGATTTGGGGA
  GCTGTGGAGGGTGCAGGAGGAAGGCATGGATTCGGAGATTGGCTGCCTCATCCGATTCTATGCAGGAAAG
  CCTCATAGCACTGGGAGCTCTGAGCGGATTCAGCTCTCGGGAATGTACAATGTCCGAAAAGGCAAAATGC
  AGTTGCCAGTGAACAGATGGACAAGACGCCAGGTCATTCTGTGTGGGACGTGCTTGATAGTGTCGTCTGT
  GAAAGACAGCTCGAGTGGGAAGATGCACGTTCTGCCTCTCATTGGTGGAAAAGTGGAAGAAGTGAAAAAG
  CACCAGCACTGTCTAGCGTTCAGCTCTTCCGGGCCCCAGAGCCAGACTTACTACATCTGCTTCGATACTT
  TCACGGAGTACTTACGATGGCTGCGTCAAGTCTCCAAGGTTGCATCACAACGCATAAGCTCAGTAGACCT
  CTCATGTTGTAGCCTTGAGCATCTGCCTGCCAACCTCTTTTACAGCCAAGACCTTACTCATCTCAATTTA
  AAACAGAACTTCCTAAGGCAGAACCCCAGCCTCCCAGCTGCCAGAGGACTTGGTGAACTGCAGAGATTCA
  CCAAATTGAAGAGCCTTAACCTTTCCAATAACCACCTAGGAGCCTTTCCGTCAGCAGTCTGCAGCATCCC
  AACCCTGGCAGAGCTGAATGTGTCTTGCAATGCCCTGCAAGAAGTCCCAGCAGCTGTTGGAGCTATGCAG
  AACTTACAGACGTTCTTGCTGGATGGAAATTTTCTCCAGTCCCTTCCTGCTGAGTTGGAGAACATGCACC
  AGCTCAGCTATTTGGGTCTTTCCTTTAATGAATTCACTGACATTCCAGAGGTATTGGAGAAGCTGACTGC
  TGTGGATAAACTGTGCATGGCTGGGAACTGTATGGAGACCCTTAGACTACAGGCCTTAAGAAGGATGCCT
  CATATTAAACACGTGGACCTAAGACTGAACATACTCAGAAAGCTTATAACAGATGAAGTGGACTTTCTGC
  AACATGTCACTCAGCTTGACCTGCGAGACAATAAACTTGGTGATCTAGATGCTATGATCTTCAACAACAT
  AGAAGTTCTGCACTGCGAGAGGAATCAGCTGGTGACACTGAACATTTGTGGCTATTTCCTAAAAGCACTC
  TATGCTTCTTCTAACGAACTCGTTCAACTTGATGTCTACCCAGTTCCAAATTATCTATCGTACATGGATG
  TCTCAAGAAACTGCCTAGAAAGTGTGCCTGAGTGGGTATGTGAAAGCCGGAAGTTAGAAGTTTTGGATAT
  TGGCCATAATCAAATATGTGAACTTCCTGCCCGCTTATTTTGTAATAGTAGTCTCCGGAAATTGCTGGCA
  GGACACAACCGGTTGGCAAGGCTTCCTGAAAGGCTGGAGAGAACATCAGTGGAGGTCTTGGACGTACAGC
  ATAACCAGATCATTGAGCTCCCACCAAACCTTCTCATGAAGGCTGACAGCCTGAGATTCCTCAATGCGTC
  CGCAAACAAACTGGAAACCCTGCCTCCAGCCACACTTTCTGAGGAGACAAGCAGTATATTACAGGAGTTG
  TACTTGACAAACAACAGCCTCACTGATAAGTGTGTGCCCTTGTTAACAGGGCACCCCCGCCTGAAGATCC
  TACACATGGCTTATAACCGGCTTCAGAGCTTCCCAGCAAGTAAAATGGCGAAACTGGAGGAGCTTGAAGA
  AATCGATATCAGTGGGAATAAGCTGAAAGCCATCCCAACAACCATCATGAACTGCAGGCGCATGCACACG
  GTGATTGCTCACTCGAACTGCATCGAAGTCTTTCCTGAAGTCATGCAGCTCCCAGAAGTCAAGTGTGTAG
  ATCTGAGCTGTAATGAGCTGAGTGAAATCACCCTACCAGAAAACCTGCCACCTAAACTGCAGGAACTAGA
  TCTGACCGGAAACCCACGCCTTGCCCTGGACCACAAAAGCTTGGAGCTGCTCAATAACATCCGCTGTTTC
  AAGATTGACCAGCCGTCAGCTGGAGATGCATCTGGAGCCCCAGCAGTATGGAGTCATGGTTACACTGAAG
  CATCGGGAGTAAAAAACAAGCTGTGTGTCGCAGCCCTGTCTGTGAATAACTTCCGTGACAACCGAGAGGC
  TCTCTACGGTGTGTTTGATGGAGACCGGAATGTGGAAGTGCCCTACCTTCTCCAGTGCACCATGAGTGAC
  ATCTTAGCTGAGGAGCTTCAGAAAACAAAAAATGAAGAAGAATACATGGTCAATACATTTATAGTCATGC
  AAAGGAAACTGGGAACGGCTGGGCAGAAACTCGGTGGTGCTGCTGTCCTGTGTCACATCAGGCATGACCC
  TGTGGACCTGGGAGGATCCTTTACCCTGACCTCTGCTAATGTTGGCAAGTGCCAAACGGTTCTCTGTCGA
  AACGGGAAGCCGCTGTCTCTGTCCAGGTCCTATACCATGAGCTGTGAAGAAGAGCGGAAGAGGATTAAGC
  AGCACAAAGCCATCATCACTGAGGATGGCAAGGTCAATGGAGTGACAGAGTCCACACGCATCCTGGGCTA
  CACCTTCCTTCACCCCAGTGTGGTGCCTCGCCCCCACGTACAGTCGGTGCTTCTGACTCCACAGGATGAG
  TTTTTCATCCTGGGCAGCAAAGGGCTGTGGGACAGCCTGTCCATCGAAGAGGCTGTGGAAGCCGTGCGCA
  ACGTTCCAGATGCTCTAGCCGCCGCCAAGAAGCTATGCACCCTGGCCCAGAGCTACGGCTGCCACGACAG
  CATCAGTGCTGTGGTGGTGCAACTCAGTGTCACGGAAGACAGCTTCTGCTGCTGTGAGCTCAGTGTGGGA
  GGAAGCATGCCACCGCCCAGTCCCGGAATCTTCCCGCCCTCCGTCAGCATGGTGATCAAGGACCGGCCCT
  CAGATGGGCTGGGCGTGCCGTCCTCCAGCAGCGGCATGGCATCTGAGATCAGCAGTGAATTGTCTACCTC
  TGAGATGAGTAGTGAGGTGGGCTCCACAGCTTCTGACGAGCCGCCGTCGGGAGCCCTGAGTGAGAGCAGC
  CCCGCCTACCCCAGCGAGCAGCGCTGCATGCTCCACCCGGTCTGCCTGTCCAACTCCTTCCAGCGCCAGC
  TGTCCAGCGCCACTTTCTCCAGTGCGTTCTCTGACAACGGCCTTGACAGTGATGACGAGGAACCCATTGA
  GGGCGTGTTCAGCAACGGCAGCCGGGTGGAGGTGGAAGTGGACATCCATTGCAGCCGGGCCAAGGAGAAG
  GAGAGACAGCAGCACCTGCTTCAGGTACCAGCTGAAGCCAGTGATGAGGGCATTGTCATCAGTGCCAATG
  AGGATGAGTCAGGTCTGTCCAAGAAGACAGACATTTCTGCTGTGGGGACCATTGGGCGACGCAGGGCTAA
  CGGCTCTGTACCTCCCCAGGAAAGGAGCCATAATGTAATAGAGGTTGCCACAGATGCACCTCTCCGGAAG
  CCGGGAGGCTATTTTGCAGCCCCTGCTCAACCAGATCCAGATGATCAGTTTATCATCCCCCCAGAGCTGG
  AAGAGGAAGTCAAAGAAATCATGAAACATCACCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
  GCAGCAGCAGCAGCCACCACCTCCACCCCAGCCGCCACAGGCACAGGCACAGGCACAGGCACAGGCACAG
  AGGCCCTTCCAGATGGATCACCTGCCGGACTGTTACGACACACCACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202102 representing NM_021657
  Red=Cloning site Green=Tags(s)
  
  MEPAAAAPAQRLADPTGEDRAPAAAAAAEGGRSPDSVLSAAAPSGGNGGAAREEAPCEAPPGPLPGRAGG
  TGRRRRRGVPQPAAGGAAPVTAAGGGANSLLLRRGRLKRNLSAAASSSSSPSSASSAAGGLPASCSASAS
  LCTRSLDRKTLLQKHRQLLQLQPSDRDWVRHQLQRGCVHVFDRHMASSYLRPVLCTLDTTAAEVAARLLQ
  LGHKGGGVVKVLGHGPPPAAAPAASDQTPATELGRDVEPPPSSSTVGAVRGPARAPPADLPLPGGAWTRC
  APRVNPAPSDSSPGELFAGGPCSPSRAPRPASDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADP
  ATVLTGPSGPPHHPVRSSQPRPPSPKTSALLQPKAPTGVDGTGLVVGEGPGDDKAVAAAAPGVPLWTPGR
  IRETVQKTSSPPSLYVQLHGETTRRLEADEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGK
  PHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSSSGKMHVLPLIGGKVEEVKK
  HQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNL
  KQNFLRQNPSLPAARGLGELQRFTKLKSLNLSNNHLGAFPSAVCSIPTLAELNVSCNALQEVPAAVGAMQ
  NLQTFLLDGNFLQSLPAELENMHQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMAGNCMETLRLQALRRMP
  HIKHVDLRLNILRKLITDEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNICGYFLKAL
  YASSNELVQLDVYPVPNYLSYMDVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLA
  GHNRLARLPERLERTSVEVLDVQHNQIIELPPNLLMKADSLRFLNASANKLETLPPATLSEETSSILQEL
  YLTNNSLTDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEEIDISGNKLKAIPTTIMNCRRMHT
  VIAHSNCIEVFPEVMQLPEVKCVDLSCNELSEITLPENLPPKLQELDLTGNPRLALDHKSLELLNNIRCF
  KIDQPSAGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSD
  ILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIRHDPVDLGGSFTLTSANVGKCQTVLCR
  NGKPLSLSRSYTMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDE
  FFILGSKGLWDSLSIEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSVG
  GSMPPPSPGIFPPSVSMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPSGALSESS
  PAYPSEQRCMLHPVCLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEK
  ERQQHLLQVPAEASDEGIVISANEDESGLSKKTDISAVGTIGRRRANGSVPPQERSHNVIEVATDAPLRK
  PGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQQQQQQQQQQPPPPPQPPQAQAQAQAQAQ
  RPFQMDHLPDCYDTPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021657
• ORF Size: 5088 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021657.1, NP_067689.1
• RefSeq Size: 6251
• RefSeq ORF: 5091
• Locus ID: 59265
• MW: 183.4 kDa
• Gene Summary: a circadian rhythm protein; may have a role in the intracellular signaling in the suprachiasmatic nucleus [RGD, Feb 2006]