Phkb (NM_001014152) Rat Tagged ORF Clone

CAT#: RR202189

Phkb (Myc-DDK-tagged ORF) - Rat phosphorylase kinase, beta (Phkb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phkb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202189 representing NM_001014152
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGGAAGAGGGACTCATGTCTGAAGTAAGCTGGAAGCTCTTGGAACGACGAGCCCGGGCCAAGC
  GCTCAGGCTCAGTTTATGAACCTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTATGGGA
  TAAGTTGGATCATTATTACAGAATTGTCAAGTCAACGATGCTGACATATCAAAGCCCAACTACAGGTCTC
  TTTCCTACTAAAACTTGTGGTGGAGATCAGAAGTCCAAAGTGCATGAGAGCCTGTACTGTGCTGCGGGTG
  CTTGGGCCTTGGCTCTGGCGTACAGGCGCATTGATGATGACAAGGGAAGGACCCATGAGCTGGAGCACTC
  GGCCATAAAGTGTATGAGAGGAGTTCTCTACTGCTACATGCGGCAGGCTGATAAGGTCCAGCAGTTCAAG
  CAGGATCCCCGGCCCACGACGTGTCTACACTCTGTTTTCAATGTGCACACTGGGGATGAAGTGCTCTCCT
  ATGAAGAATATGGCCATCTTCAGATAAATGCAGTCTCACTCTTTCTCCTTTACCTGGTGGAAATGATTTC
  CTCAGGTCTCCAGATAATCTATAACACGGATGAGGTCTCTTTTATTCAAAACCTTGTGTTTTGTGTAGAG
  AGAGTTTACCGTGTTCCCGACTTCGGTGTCTGGGAAAGAGGAAGCAAATATAACAACGGCAGCACAGAAC
  TGCACTCGAGCTCTGTTGGTTTGGCAAAAGCAGCCTTAGAAGCGATTAATGGATTTAACCTCTTTGGAAA
  CCAGGGCTGTTCTTGGTCAGTTATATTCGTGGATCTCGATGCTCACAACCGCAACAGGCAGACGTTGTGT
  TCACTGTTACCCAGAGAATCACGCTCTCATAACACAGATGCTGCCCTGCTCCCGTGCATCACCTACCCCG
  CCTTTGCCCTGGACGATGAAGCTCTGTTCAGCCAGACACTCGATAAAGTGATAAGAAAATTAAAAGGGAA
  ATATGGATTTAAGCGTTTCTTGCGAGATGGGTACAGAACGCCCCTGGAAGACCCCAACAGACGCTACTAC
  AAACCAGCGGAGATTAAGCTGTTTGATGGCATCGAATGTGAATTTCCCATATTCTTCCTTTACCTGATGA
  TCGATGGTGTTTTTAGAGGCAATCTTGAACAAGTCAAGGAATATCAGGACCTGTTGACACCTTTGCTTCA
  CCAGACCACAGAAGGATATCCTGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAA
  AAAAGAAACCCTGGTAGTCAGAAGCGGTTTCCTAGTAACTGTGGCCGTGATGGGAAATTGTTTCTTTGGG
  GACAAGCTCTTTATATCATTGCAAAACTCCTAGCTGATGAGTTAATCAGTCCTAAAGACATTGACCCCGT
  CCATCGCTTTGTCCCACTTCAGAATCAACGTAACGTGAGCATGAGATTCTCTAATCAGGGCCCACTGGAA
  AATGACTTGGTGGTTCATGTGGCACTTGTAGCAGAAAGCCAACGCCTTCAGGTCTTCCTCAATACTTACG
  GGATTCAGACTCAAACGCCCCAGCAGGTGGAGCCTATTCAGATCTGGCCTCAGCAGGAGCTCGTGAAAGC
  TTATTTCCACCTGGGTATCAATGAAAAGTTGGGTCTGTCTGGAAGGCCAGATAGGCCCATTGGCTGCCTT
  GGTACTTCAAAGATTTACCGCATCCTAGGAAGGACCGTGGTTTGTTACCCTATCATCTTCGACCTCAGTG
  ATTTCTACATGTCTCAGGACGTTCTCCTGCTCATAGATGACATAAAGAATGCACTACAGTTCATTAAGCA
  GTATTGGAAAATGCATGGACGCCCACTTTTTCTTGTTCTCATCCGGGAAGACAACATAAGAGGTAGCAGA
  TTCAACCCCATATTAGACATGCTGGCAGCCTTTAAAAAGGGGATAATTGGAGGAGTCAAAGTTCATGTTG
  ACCGTTTACAGACATTGATATCTGGAGCTGTAGTAGAACAGCTTGACTTTCTGCGGATTAGCGACACAGA
  AAAGCTTCCAGAGTTTAAGAGCTTTGAAGAACTAGAATTTCCCAAGCATTCAAAAGTCAAACGACAGAGC
  AGTACTGCAGATGCTCCTGAACTGCGACAGGAGCCAGACATCACCATCACAGAGTGGAAAAACAAATCCA
  CCCACGAAATCCTTCAGAAGCTTAATGACTGCGGTTGTCTGGCTGGCCAAACCATCCTGCTGGGTATACT
  GCTCAAAAGAGAGGGCCCTAACTTCATCACGATGGAAGGAACTGTGTCTGATCACATTGAGAGGGTGTAC
  AGAAGAGCTGGCAGCAAAAAGCTTTGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAATAAAGTAGTGG
  ACAGCCTGGCCCCATCCATTACTAATGTTTTAGTGCAGGGCAAACAGGTAACCCTAGGTGCCTTTGGACA
  CGAAGAGGAGGTCATCTCTAATCCACTATCTCCAAGAGTGATTAAGAACATCATCTATTATAAGTGTAAC
  ACCCATGATGAGAGGGAGGCTGTCATTCAGCAAGAGCTGGTCATCCATATTGGCTGGATCATCTCCAACA
  ACCCCGAGCTGTTCAGTGGCATGCTGAAAATCAGAATTGGGTGGATCATCCATGCCATGGAGTACGAACT
  GCAGATCCGTGGCGGGGATAAGCCAGCTGTGGACTTGTACCAGCTCTCGCCCAGTGAGGTTAAGCAGCTG
  CTGCTCGACATCCTGCAGCCTCAGCAGAGTGGGAGATGTTGGCTGAATAGACGTCAGATTGATGGGTCAC
  TGAATAGAACACCCCCAGGATTCTACGACCGCGTGTGGCAGATCCTGGAGCGCACACCTAATGGTATCGT
  TGTAGCTGGCAAGCATTTGCCACAGCAACCAACCTTGTCAGATATGACCATGTATGAGATGAATTTTTCT
  CTCCTTGTTGAAGATATGTTGGGAAACATTGACCAGCCAAAGTATAGACAGATCATTGTAGAGTTACTAA
  TGGTTGTATCCATTGTGCTGGAAAGAAATCCTGAGCTAGAATTTCAAGACAAAGTGGATCTGGACAAACT
  GGTCAAAGAAGCGTTTCATGAGTTTCAAAAAGATGAGAGCCGGCTAAAAGAAATCGAAAAGCAAGTAAGT
  GGAGAGGCCCGTGTCCCACAGAACGCTTTCATTGTTTCCACTGTTGAAATCCTCTCTAATCTTCCCACTT
  TCCAGACAGATGGACAGAGCACAAAAGAGGATGCTCTACAAGCAGTCAGGCTTACTGAGGAATTAATATT
  GCTTTTTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202189 representing NM_001014152
  Red=Cloning site Green=Tags(s)
  
  MAGEEGLMSEVSWKLLERRARAKRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTMLTYQSPTTGL
  FPTKTCGGDQKSKVHESLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGVLYCYMRQADKVQQFK
  QDPRPTTCLHSVFNVHTGDEVLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVE
  RVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLC
  SLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLRDGYRTPLEDPNRRYY
  KPAEIKLFDGIECEFPIFFLYLMIDGVFRGNLEQVKEYQDLLTPLLHQTTEGYPVVPKYYYVPADFVEYE
  KRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVHRFVPLQNQRNVSMRFSNQGPLE
  NDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCL
  GTSKIYRILGRTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSR
  FNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFKSFEELEFPKHSKVKRQS
  STADAPELRQEPDITITEWKNKSTHEILQKLNDCGCLAGQTILLGILLKREGPNFITMEGTVSDHIERVY
  RRAGSKKLWSVVRRAASLLNKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCN
  THDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPAVDLYQLSPSEVKQL
  LLDILQPQQSGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMTMYEMNFS
  LLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQVS
  GEARVPQNAFIVSTVEILSNLPTFQTDGQSTKEDALQAVRLTEELILLFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014152
• ORF Size: 3300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014152.1, NP_001014174.1
• RefSeq Size: 3630 bp
• RefSeq ORF: 3303 bp
• Locus ID: 361377
• Cytogenetics: 19p11
• MW: 125.9 kDa
• Gene Summary: expression in regenerating muscle may be correlated with that of other phosphorylase kinase subunits [RGD, Feb 2006]