Galt (NM_001013089) Rat Tagged ORF Clone

CAT#: RR202350

Galt (Myc-DDK-tagged ORF) - Rat galactose-1-phosphate uridylyltransferase (Galt), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galt
• Synonyms: MGC112778
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202350 representing NM_001013089
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCAAAGCGGAGCCGATCCTGAGCAGCGCCAGCAGGCTTCAGAGGCGGACGCCATGGCAGCGACCT
  TCCGGGCGAGCGAACACCAGCATATTCGCTACAACCCGCTCCAGGATGAGTGGGTGTTAGTGTCAGCCCA
  TCGCATGAAGCGGCCCTGGCAAGGACAAGTGGAGCCCCAGCTTCTGAAGACGGTCCCCCGCCATGACCCA
  CTCAACCCTCTGTGTCCCGGGGCCACACGAGCTAATGGGGAGGTGAATCCCCCCTATGATGGCACCTTCT
  TATTTGACAATGACTTCCCGGCTCTGCAGCCCGATGCTCCGGATCCAGGACCCAGTGACCACCCTCTCTT
  CCGAGTGGAGGCCGCCAGAGGAGTTTGTAAGGTCATGTGCTTCCACCCCTGGTCGGATGTGACGCTGCCA
  CTCATGTCTGTCCCTGAGATCCGAGCTGTCATCGATGCATGGGCCTCAGTCACAGAGGAACTGGGCGCCC
  AGTACCCTTGGGTGCAGATCTTTGAAAACAAAGGAGCCATGATGGGTTGCTCTAACCCCCATCCCCACTG
  CCAGGTTTGGGCTAGCAATTTCCTGCCAGATATTGCCCAGCGTGAAGAGCGATCCCAGCAGACTTATCAC
  AACCAGCATGGAAAGCCTTTATTATTGGAATATGGCCACCAAGAGCTCCTCAGGAAGGAACGTCTGGTCC
  TAACCAGTGAGTACTGGATAGTTCTGGTCCCCTTCTGGGCAGTGTGGCCTTTCCAGACATTACTGCTGCC
  CCGACGGCACGTGCAGCGGCTACCTGAGCTGACCCCTGCTGAGCGTGATGATCTAGCCTCCACCATGAAG
  AAGCTCTTGACCAAGTATGACAACCTGTTTGAGACGTCCTTTCCTTACTCCATGGGCTGGCACGGGGCTC
  CCATGGGATTAAAGACTGGAGCCACCTGTGACCACTGGCAGCTCCATGCTCACTATTACCCCCCACTCCT
  GCGATCGGCTACTGTTCGGAAATTCATGGTTGGCTATGAAATGCTTGCCCAGGCCCAGCGTGACCTCACT
  CCCGAACAGGCTGCAGAAAGATTAAGGGTGCTCCCTGAGGTACACTATTGCCTGACGCAGAAAGACAAGG
  AAACAGCAGCCACCGCTAAACAGCAGCCACCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202350 representing NM_001013089
  Red=Cloning site Green=Tags(s)
  
  MSQSGADPEQRQQASEADAMAATFRASEHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDP
  LNPLCPGATRANGEVNPPYDGTFLFDNDFPALQPDAPDPGPSDHPLFRVEAARGVCKVMCFHPWSDVTLP
  LMSVPEIRAVIDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASNFLPDIAQREERSQQTYH
  NQHGKPLLLEYGHQELLRKERLVLTSEYWIVLVPFWAVWPFQTLLLPRRHVQRLPELTPAERDDLASTMK
  KLLTKYDNLFETSFPYSMGWHGAPMGLKTGATCDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLT
  PEQAAERLRVLPEVHYCLTQKDKETAATAKQQPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013089
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1375 bp
• RefSeq ORF: 1140 bp
• Locus ID: 298003
• Cytogenetics: 5q22
• MW: 43.3 kDa