Alpi (NM_022665) Rat Tagged ORF Clone

CAT#: RR202370

Alpi (Myc-DDK-tagged ORF) - Rat alkaline phosphatase, intestinal (Alpi), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alpi
• Synonyms: Alp1; S51097
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202370 representing NM_022665
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGAGACTGGGTGCTGCTGCTGCTTTTGGGTCTCAGGATACATCTGTCCTTTGGTGTCATCCCAG
  TGGAGGAGGAGAACCCAGTCTTCTGGAATCAAAAGGCGAAGGAGGCCCTGGATGTTGCCAAAAAGCTGCA
  GCCCATTCAGACATCGGCCAAGAACCTCATCCTCTTCCTGGGAGATGGGATGGGGGTACCCACAGTGACA
  GCCACCAGGATACTAAAGGGACAACTGGGTGGTCATCTAGGACCTGAGACACCCCTGGCCATGGACCACT
  TCCCATTCACGGCTCTGTCCAAGACATACAACGTGGACAGACAGGTCCCAGACAGTGCAGGCACAGCCAC
  CGCCTATCTCTGTGGGGTCAAAGCCAACTACAAGACCATCGGTGTGAGCGCGGCTGCTCGATTCAACCAG
  TGCAACAGCACATTTGGCAACGAGGTCTTCTCAGTGATGCACCGTGCAAAGAAAGCAGGGAAGTCTGTGG
  GCGTGGTGACCACGACCAGAGTGCAGCATGCCTCTCCAGCCGGCACCTACGCGCACACGGTTAACCGAGA
  CTGGTACTCGGATGCAGACATGCCTTCCTCCGCCCTGCAGGAGGGCTGCAAGGACATCGCCACACAGCTC
  ATTTCCAACATGGACATTGATGTGATCCTTGGTGGAGGCCGAAAGTTCATGTTTCCCAAGGGGACACCAG
  ACCCTGAATACCCAGGTGACTCTGACCAGTCTGGAGTCAGGCTGGATAGTCGGAACTTGGTGGAGGAGTG
  GCTGGCAAAATACCAGGGGACCCGGTATGTTTGGAATCGAGAGCAGCTCATGCAGGCCTCTCAGGACCCA
  GCAGTGACTCGCCTCATGGGCCTCTTTGAGCCGGCAGAAATGAAATATGATGTCAACCGAAACGCCTCAG
  CAGACCCCTCCCTGGCAGAGATGACAGAGGTGGCCGTGCGCCTGCTGAGCAGGAATCCACAAGGCTTCTA
  CCTCTTTGTAGAAGGAGGTCGTATTGACCAAGGCCACCATGCGGGCACGGCTTACCTGGCACTGACTGAG
  GCTGTCATGTTCGACTCCGCCATCGAAAAGGCCAGCCAGCTTACCAATGAGAAGGACACGCTAACGCTCA
  TCACTGCGGACCACTCCCATGTCTTTGCTTTTGGTGGCTATACACTTAGGGGGACCTCCATCTTTGGTCT
  GGCCCCACTCAATGCCCAGGATGGCAAATCCTACACCTCCATTCTCTATGGCAACGGCCCAGGTTATGTG
  CTTAATTCAGGCAACCGGCCCAATGTCACTGATGCCGAGAGTGGTGATGTCAACTACAAGCAGCAGGCAG
  CAGTACCCCTGTCGTCAGAGACCCACGGTGGGGAGGACGTGGCCATATTCGCGCGTGGCCCACAGGCGCA
  CCTGGTGCACGGAGTGCAGGAACAGAACTACATCGCGCACGTCATGGCCTTCGCAGGCTGCCTGGAGCCC
  TACACCGACTGCGGTCTGGCGCCCCCTGCAGACGAGAACAGACCAACCACCCCGGTCCAGAACAGTGCCA
  TCACTATGAACAATGTCCTTCTGTCCCTGCAGCTGCTGGTCAGCATGCTGCTGCTAGTCGGGACAGCCTT
  GGTGGTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202370 representing NM_022665
  Red=Cloning site Green=Tags(s)
  
  MQGDWVLLLLLGLRIHLSFGVIPVEEENPVFWNQKAKEALDVAKKLQPIQTSAKNLILFLGDGMGVPTVT
  ATRILKGQLGGHLGPETPLAMDHFPFTALSKTYNVDRQVPDSAGTATAYLCGVKANYKTIGVSAAARFNQ
  CNSTFGNEVFSVMHRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRDWYSDADMPSSALQEGCKDIATQL
  ISNMDIDVILGGGRKFMFPKGTPDPEYPGDSDQSGVRLDSRNLVEEWLAKYQGTRYVWNREQLMQASQDP
  AVTRLMGLFEPAEMKYDVNRNASADPSLAEMTEVAVRLLSRNPQGFYLFVEGGRIDQGHHAGTAYLALTE
  AVMFDSAIEKASQLTNEKDTLTLITADHSHVFAFGGYTLRGTSIFGLAPLNAQDGKSYTSILYGNGPGYV
  LNSGNRPNVTDAESGDVNYKQQAAVPLSSETHGGEDVAIFARGPQAHLVHGVQEQNYIAHVMAFAGCLEP
  YTDCGLAPPADENRPTTPVQNSAITMNNVLLSLQLLVSMLLLVGTALVVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022665
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022665.1, NM_022665.2, NM_022665.3, NP_073156.3
• RefSeq Size: 2226
• RefSeq ORF: 1623
• Locus ID: 24197
• MW: 58.4 kDa
• Gene Summary: catalyzes the conversion of an orthophosphoric monoester to an alcohol phosphate [RGD, Feb 2006]