Ncl (NM_012749) Rat Tagged ORF Clone

CAT#: RR202375

Ncl (Myc-DDK-tagged ORF) - Rat nucleolin (Ncl), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncl
• Synonyms: MGC93572
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202375 representing NM_012749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAACTCGCAAAGGCCGGCAAAACCCACGGAGAGTCCAAGAAAATGGCTCCTCCTCCAAAGGAGG
  TGGAAGAAGATAGTGAGGATGAAGAAATGTCAGAAGATGAAGATGACAGCAGTGGAGAAGAGGAGGTTGT
  CATCCCTCAGAAGAAAGGCAAAAAGGCTACCACAACTCCAGCAAAGAAGGTGGTTGTTTCACAAACAAAA
  AAGGCTGCAGTTCCCACACCAGCTAAGAAAGCAGCTGTTACCCCAGGCAAAAAGGCAGCAGCCACACCAG
  CCAAGAAAGCTGTTACACCAGCCAAAGTAGTTCCAACACCTGGTAAAAAGGGAGCTGCACAAGCAAAAGC
  ATTGGTACCAACTCCTGGTAAAAAGGGAGCTGTCACTCCAGCCAAGGGGGCTAAGAATGGTAAGAATGCC
  AAGAAGGAAGACAGCGATGAGGATGAAGATGAAGAGGATGAAGATGACAGCGATGAGGATGAAGATGAAG
  AGGATGAATTTGAGCCACCGGTAGTAAAAGGAGTGAAACCAGCAAAAGCAGCTCCTGCTGCTCCTGCCTC
  AGAGGATGAGGATGAGGAAGATGATGATGATGAAGAAGATGATGATGATGATGATGAAGAGGAGGAGGAG
  GAAGATGACTCTGAGGAAGAAGTTATGGAGATCACACCAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTG
  TTCCTGTGAAAGCCAAGAGTGTGGCCGAGGAGGAGGAAGATGATGAGGATGATGAAGATGAAGAGGAGGA
  TGAAGATGAAGAAGATGAAGAGGACGATGAAGATGAGGATGAGGAAGAAGAGGAAGAACCTGTTAAAGCA
  GCACCTGGAAAACGGAAGAAGGAGATGACCAAGCAGAAAGAAGCCCCTGAAGCCAAGAAACAGAAAATAG
  AAGGCTCAGAACCAACTACACCTTTCAACCTGTTCATTGGAAACCTTAATCCAAACAAGTCTGTTGCTGA
  ATTAAAAGTTGCCATCAGTGAACTTTTTGCTAAAAATGATCTTGCTGCTGTGGATGTCAGAACTGGTACA
  AATAGGAAATTTGGTTATGTTGATTTTGAGTCTGCTGAAGACCTAGAAAAGGCCCTGGAGCTCACTGGTT
  TAAAAGTGTTTGGCAATGAAATTAAACTAGAAAAACCAAAAGGAAGAGATAGTAAGAAAGTTCGAGCTGC
  AAGAACACTTTTAGCCAAAAACCTCTCTTTCAACATCACTGAGGATGAATTAAAAGAAGTGTTTGAAGAT
  GCTGTGGAGATCAGATTAGTCAGCCAGGATGGGAGAAGTAAAGGGATTGCTTATATTGAATTTAAGTCTG
  AGGCTGATGCAGAGAAAAACTTGGAAGAAAAGCAGGGGGCAGAAATTGATGGACGGTCTGTTTCACTCTA
  CTACACTGGAGAGAAAGGACAAAGGCAAGAGAGAACTGGAAAGAATAGCACTTGGAGTGGTGAATCAAAG
  ACTTTGGTTTTAAGTAACCTTTCCTACAGTGCAACAGAAGAAACACTTCAGGAAGTATTCGAGAAAGCAA
  CCTTTATTAAAGTGCCCCAGAACCCACATGGCAAATCTAAAGGGTATGCATTTATAGAATTTGCTTCATT
  TGAAGATGCTAAAGAAGCTTTAAATTCCTGTAATAAAATGGAAATTGAGGGCAGAACAATCAGGCTGGAG
  TTGCAAGGACCCAGGGGATCACCTAATGCGAGAAGTCAGCCATCCAAAACTCTGTTTGTCAAAGGTCTGT
  CTGAGGATACCACTGAAGAGACCTTAAAAGAATCATTTGAGGGCTCTGTTCGTGCAAGAATAGTAACTGA
  TCGGGAAACTGGTTCTTCTAAAGGGTTTGGTTTTGTAGACTTTAATAGTGAGGAAGATGCCAAAGCTGCC
  AAGGAGGCCATGGAAGATGGAGAAATTGATGGAAACAAAGTTACCTTGGACTGGGCCAAACCTAAGGGTG
  AAGGTGGCTTTGGTGGTCGAGGTGGAGGCAGAGGAGGTTTCGGAGGCAGAGGTGGTGGCAGAGGCGGAAG
  AGGCGGATTTGGCGGAAGAGGCCGGGGAGGCTTTGGAGGCAGAGGAGGCTTCCGAGGCGGCAGAGGAGGC
  GGGGGAGACTTCAAGCCACAAGGAAAGAAGACGAAGTTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202375 representing NM_012749
  Red=Cloning site Green=Tags(s)
  
  MVKLAKAGKTHGESKKMAPPPKEVEEDSEDEEMSEDEDDSSGEEEVVIPQKKGKKATTTPAKKVVVSQTK
  KAAVPTPAKKAAVTPGKKAAATPAKKAVTPAKVVPTPGKKGAAQAKALVPTPGKKGAVTPAKGAKNGKNA
  KKEDSDEDEDEEDEDDSDEDEDEEDEFEPPVVKGVKPAKAAPAAPASEDEDEEDDDDEEDDDDDDEEEEE
  EDDSEEEVMEITPAKGKKTPAKVVPVKAKSVAEEEEDDEDDEDEEEDEDEEDEEDDEDEDEEEEEEPVKA
  APGKRKKEMTKQKEAPEAKKQKIEGSEPTTPFNLFIGNLNPNKSVAELKVAISELFAKNDLAAVDVRTGT
  NRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGRDSKKVRAARTLLAKNLSFNITEDELKEVFED
  AVEIRLVSQDGRSKGIAYIEFKSEADAEKNLEEKQGAEIDGRSVSLYYTGEKGQRQERTGKNSTWSGESK
  TLVLSNLSYSATEETLQEVFEKATFIKVPQNPHGKSKGYAFIEFASFEDAKEALNSCNKMEIEGRTIRLE
  LQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFEGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAA
  KEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGG
  GGDFKPQGKKTKFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012749
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012749.1, NM_012749.2, NP_036881.2
• RefSeq Size: 2501
• RefSeq ORF: 2145
• Locus ID: 25135
• MW: 77.3 kDa
• Gene Summary: nucleolar-specific protein; involved in cell growth [RGD, Feb 2006]