Ankzf1 (NM_001014219) Rat Tagged ORF Clone

CAT#: RR202381

  • TrueORF®

Ankzf1 (Myc-DDK-tagged ORF) - Rat ankyrin repeat and zinc finger domain containing 1 (Ankzf1), (10 ug)


  "NM_001014219" in other vectors (3)

Reconstitution Protocol

USD 970.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Ankzf1
Synonyms RGD1359242
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR202381 representing NM_001014219
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCCAGCTCCACGTGCAGCCCCAGTCCCTGCCTCGGTTTCCCTGTTGACCCTCAACGGGGAAGCTC
CACTTGTTCGGGGCCTGAGCCTGGTGAGCCAAGCTCCCGGGGAGGCTCTGGCCTGGGCGCCGCGTACTTC
CTGTCCAGGAGAGAACACAAGCTCAGGGAGAAAAGTATCTCCATGTTCTCTGGATATTTCAGATAAATTA
TTTTGTTCAGCCTGTGACCAGGTCTTCCAGAACCACCAGGAACAGAGAGAACATTATAAACTTGATTGGC
ATCGCTTTAACCTAAAACAACGTCTCAAGAATAAGCCTCTCCTATCTGCTTCAGACTTTGAAAGGCAAAG
CTCCAGAGGAGATCTTTCCAGCATCTCAGGATCAGAAGACTCAGACTCAGCCAGTGAAGAGGACTTGCTG
ACACTGGATGAGGAGAGGGCTGAATCTGAAAAACCCAACCGACCCCCTGGATTCTACCCACATCGGGTCC
TTTTCAGAAACGCCCAGGGCCAGTTTCTTTATGCCTATCGTTGTGTCCTAGGCCCTCACCAGATTCCTCC
AGAAAAGGCAGAATTGCTGCTACAAAACCTGCAAAATGGAGGTCCCAGACACTACGTGGTGCTCATGGCT
GCAGCTGGGCATTTTGCTGGTGCCATTTTCCAAGGAAGAGAAGTGGTGGCACACAAAACCTTTCACCGTT
ACACTGTGCGGGCCAAGCGGGGCACAGCCCAGGGCCTTCAGGATGCCCAGGGTAGGGCATCCCGCTCTGC
TGGAGCCAACTTGAGGCGTTACAATGAAGCCATGCTCTACAAGGATGTTCGTGACCTGCTGGCAGGGCCA
ACCTGGTCCAAAGCACTAGGAGAGGCAGAAACAATACTGCTGCATGCCCCACGCTCTGGCCGGTCCTTGT
TCTTTGGAGGCCAAGGGGCACCCCTACAAAGGAGTGATTTCCGACTTTGGGATATCCCTCTCACCACCCG
CAGACCCACTTTTGGAGAGCTTCAGCGTGTACTCCATAAGCTGACCACCTTGCAGGTGTATGATGAAGAC
CCTCGAGAAATGGTCAGATTTCACTCTCCTGAGACACACTGCAAACCAGTGAGAGAAGAGAGGAAAAGGG
ATACTGAGAAAGAAAAAACAAAGGTCCCTAGTGATGCAAATAAGGCACTTGGGCAGGATGAAGAACCACT
CAAACAAGGTTCCGAGTCTCAGGAAGAAGATGGCTCCGAAGTAGAGTTGGAGCTAGTAGAGCTGACACTG
GGGACCCTGGATCTTCGTGAATTTGAAGTATTGCCCAAGCGGAGGAAGAAGAGGAAAAAGAAGGAGAGAA
GTCAAAAGCAGCAATGTGGGGCACATGGGCCCCTTCTTCAGCAACCTCAAGATGAGCCATTCTCACAGCC
AACCCAAGTGGTTGCAACCCCATTGGACACTTTGGTCGATGAGGCCAAAGCCCCTGGTCAACCAGAGCTC
TGGGATATGCTTCTAGCTGCTTGTCGTGCTGGGGAAGTTGAGGTGCTAAAGCTGCAGCTAGCCACTGGGC
CTGTAGACCCTGGAGTTATGTCCCTACTCAGTGCCCCTTTGGGCTCTGGTGGCTTTACTCTCCTGCATGC
AGCAGCCGCAGCTGGAAGAGGCTTAGTAGTTCGTTTGCTGTTGGAGGCAGGTGCTGACCCCACTGTGCAT
GACTCTAGGGCTCGGCCACCTTATACTGTAGCAGCTGACAGATCAACGCGTAATGAATTCCGGAGGTTCA
TGGAGAAGAATCTTGATGCTTATGATTACAGCAAGGCTCGGGTGCCAGGGCCATTGACTCAAGAAATGGA
GGCCCGGCAGGCTACAAGGAAAAAGGAGCAGAAGGCAGCTCGACGGCAGCGGGAGCAACAGCAGCGGAAG
CAGAGGGAACAGGAAGAACAGGAGCAAGAGGAGCGAAGGCGGTTTGCTGCCCTCAGTGACCGAGAGAAGA
GAGCTCTGGCTGCAGAGCGCCGACTTGCTGCCCAGTTGGGAGCCCCTAGCCCTCCAGTTCCTGACTCTGC
AGTTGTCAATGCTGGGCGCTGCTGGAGTTGTGGTGTGTCCCTCCAAGGCCTCATTCCCTTTCACTACCTC
GATTTCTCTTTCTGCTCCACACGTTGCCTTCGAGACCATCGCAGTCAGGCAGGGAGGCCGTCATCA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RR202381 representing NM_001014219
Red=Cloning site Green=Tags(s)

MLPAPRAAPVPASVSLLTLNGEAPLVRGLSLVSQAPGEALAWAPRTSCPGENTSSGRKVSPCSLDISDKL
FCSACDQVFQNHQEQREHYKLDWHRFNLKQRLKNKPLLSASDFERQSSRGDLSSISGSEDSDSASEEDLL
TLDEERAESEKPNRPPGFYPHRVLFRNAQGQFLYAYRCVLGPHQIPPEKAELLLQNLQNGGPRHYVVLMA
AAGHFAGAIFQGREVVAHKTFHRYTVRAKRGTAQGLQDAQGRASRSAGANLRRYNEAMLYKDVRDLLAGP
TWSKALGEAETILLHAPRSGRSLFFGGQGAPLQRSDFRLWDIPLTTRRPTFGELQRVLHKLTTLQVYDED
PREMVRFHSPETHCKPVREERKRDTEKEKTKVPSDANKALGQDEEPLKQGSESQEEDGSEVELELVELTL
GTLDLREFEVLPKRRKKRKKKERSQKQQCGAHGPLLQQPQDEPFSQPTQVVATPLDTLVDEAKAPGQPEL
WDMLLAACRAGEVEVLKLQLATGPVDPGVMSLLSAPLGSGGFTLLHAAAAAGRGLVVRLLLEAGADPTVH
DSRARPPYTVAADRSTRNEFRRFMEKNLDAYDYSKARVPGPLTQEMEARQATRKKEQKAARRQREQQQRK
QREQEEQEQEERRRFAALSDREKRALAAERRLAAQLGAPSPPVPDSAVVNAGRCWSCGVSLQGLIPFHYL
DFSFCSTRCLRDHRSQAGRPSS

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001014219
ORF Size 2166 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001014219.1, NP_001014241.1
RefSeq Size 2702 bp
RefSeq ORF 2169 bp
Locus ID 363255
Cytogenetics 9q33
MW 80.6 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.