Gpt2 (NM_001012057) Rat Tagged ORF Clone

CAT#: RR202515

Gpt2 (Myc-DDK-tagged ORF) - Rat glutamic pyruvate transaminase (alanine aminotransferase) 2 (Gpt2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpt2
• Synonyms: ALT2; Cc2-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202515 representing NM_001012057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGGCAGCGGTGCTGGTGCGGCGGGGCTCCTGCCCCCGCGCCTCGGGCCCCTGGGGCCGTAGTC
  ACAGCAGCGCTGCAGCCGAAGCCTCGGCGGCGCTCAAGGTGCGACCAGAGCGTAGCCCTCGAGACCGCAT
  CCTCACCCTGGAGTCCATGAACCCGCAGGTGAAGGCCGTGGAGTACGCTGTGCGGGGACCCATCGTGCTC
  AAGGCCGGCGAGATCGAGATGGAGCTGCAGCGGGGTATCAAAAAACCATTCACTGAGGTAATCCGAGCCA
  ACATTGGGGATGCCCATGCTATGGGCCAGCAGCCAATCACCTTCCTTCGTCAGGTGATGGCTCTCTGCAC
  CTACCCGAACCTGCTAAACAGCCCCAGCTTCCCGGAAGATGCTAAGAAGCGAGCGCGGCGGATCCTGCAG
  GCTTGCGGTGGAAACAGCCTGGGGTCTTACAGTGCGAGCCAGGGCGTTAACTGTATCCGAGAGGACGTGG
  CAGCCTTTATCACCAGGAGAGATGGTGTACCTGCAGACCCAGACAACATTTACCTGACTACTGGAGCGAG
  CGACGGTATTTCTACGATCCTGAAGCTCCTGGTCTCCGGCGGTGGCAAGTCACGGACCGGGGTGATGATC
  CCCATCCCACAGTACCCCTTGTACTCCGCGGTCATCTCCGAGCTCGACGCCATCCAGGTGAACTACTATC
  TGGATGAGGACAACTGCTGGGCTTTGAACGTGGACGAGCTCCGGCGGGCACTGCGGCAAGCTAAAGACCA
  CTGTGACCCTAAAGTTCTCTGCATCATCAACCCCGGAAACCCCACAGGCCAGGTACAAAGCAGAAAGTGC
  ATAGAAGATGTGATTCACTTTGCCTGGGAAGAGAAGCTTTTTCTCCTGGCTGACGAGGTATACCAGGACA
  ACGTGTACTCTCCGGACTGCAGATTCCACTCCTTTAAGAAAGTGCTTTACCAGATGGGGCCTGAGTACTC
  GAGTAATGTGGAGCTTGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAATGCGGCTACAGAGGA
  GGCTACATGGAGGTGATCAATTTGCACCCCGAGATCAAAGGCCAGCTGGTTAAGCTCCTCTCGGTCCGCC
  TCTGTCCGCCAGTGTCTGGACAGGCCGCGATGGACATTGTTGTGAATCCACCGGTGCCAGGAGAGGAGTC
  CTTTGAGCAATTCACCAGGGAAAAAGAATCCGTCCTTGGTAATCTGGCCAAAAAAGCAAAGCTGACAGAA
  GATCTGTTTAACCAAGTCCCGGGGATCCAGTGCAACCCCTTGCAAGGAGCCATGTATGCATTCCCTCGGA
  TTCTCATCCCTGCCAAGGCCGTGGAGGCAGCTCAGTCCCATAAAATGGCTCCAGATATGTTTTACTGCAT
  GAAACTCCTGGAAGAAACTGGCATCTGTGTCGTGCCTGGCAGTGGCTTTGGACAGCGAGAAGGCACCTAC
  CACTTCAGAATGACAATTCTCCCTCCAGTGGAGAAACTGAAGACAGTACTCCACAAGGTGAAAGATTTTC
  ACCTGAAGTTCCTGGAGAAGTACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202515 representing NM_001012057
  Red=Cloning site Green=Tags(s)
  
  MQRAAVLVRRGSCPRASGPWGRSHSSAAAEASAALKVRPERSPRDRILTLESMNPQVKAVEYAVRGPIVL
  KAGEIEMELQRGIKKPFTEVIRANIGDAHAMGQQPITFLRQVMALCTYPNLLNSPSFPEDAKKRARRILQ
  ACGGNSLGSYSASQGVNCIREDVAAFITRRDGVPADPDNIYLTTGASDGISTILKLLVSGGGKSRTGVMI
  PIPQYPLYSAVISELDAIQVNYYLDEDNCWALNVDELRRALRQAKDHCDPKVLCIINPGNPTGQVQSRKC
  IEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYQMGPEYSSNVELASFHSTSKGYMGECGYRG
  GYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVPGEESFEQFTREKESVLGNLAKKAKLTE
  DLFNQVPGIQCNPLQGAMYAFPRILIPAKAVEAAQSHKMAPDMFYCMKLLEETGICVVPGSGFGQREGTY
  HFRMTILPPVEKLKTVLHKVKDFHLKFLEKYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012057
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012057.1, NM_001012057.2, NP_001012057.1
• RefSeq Size: 1769 bp
• RefSeq ORF: 1569 bp
• Locus ID: 307759
• Cytogenetics: 19p11
• MW: 57.8 kDa