Polr1b (NM_031773) Rat Tagged ORF Clone

CAT#: RR202655

Polr1b (Myc-DDK-tagged ORF) - Rat polymerase (RNA) I polypeptide B (Polr1b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polr1b
• Synonyms: RPA2; Rpo1-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202655 representing NM_031773
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTCGACAGCCGGTGGCGGAACCTGCCCAGCGGGCCCAGCCTAAAGCATTTAACCGACCCCTCGT
  ACGCGGTTCCTCCGGAGCAGCAAAAGGCGGCGTTGCAGGACCTGACGCGGGCGCACGTGGACTCCTTCAA
  CTACGCAGTGCTGGAGGGGCTGAGCCACGCGGTGCAGGCCATACCTCCCTTCGAATTTGCTTTCAAAGAT
  GAGCGCATATCTCTTACTATTGTGGACGCTGCCATCAGTCCACCGGCAGTGCCCAAAGGGACCATCTGCA
  AAGAGCTCAACATTTATCCAGCTGAGTGCCGTGGCCGGAGGAGCACGTACCGAGGGAAGCTGACGGCTGA
  TATCAGCTGGGCCGTGAATGGAGTCCCCAAAGGCATCATTAAACAATTTCTTGGCWATGTTCCCATCATG
  GTGAAGTCCAAGCTTTGCAACTTATACAACCTTCCTCCTCAAGTCCTCATTGAGCACCACGAGGAGGCAG
  AGGAAATGGGCGGTTATTTTATAATCAACGGCATTGAGAAAGTCATCCGCATGTTGATTATGCCTCGGAG
  AAATTTTCCCATCGCAATGATAAGACCGAAATGGAAAAGCAGAGGGCTCGGCTACACTCAGTTCGGGGTT
  TCCATTCACTGTGTGAGAGAGGAGCACTCTGCTGTCAATATGAACCTTCACTATGTGGAGAACGGCACGG
  TCATGTTAAACTTTATTTACCGCAAAGAGCTGTTTTTCCTTCCTTTGGGATTTGCACTTAAGGCACTTGT
  GAGCTTTTCTGACTATCAGATTTTCCAGGAGCTCATCAAAGGCAAAGAGGAGGACTCTTTCTTTAAGAAT
  TCTGTTTCTCAGATGCTGAGGATTGTAATGGAGGAGGGTTGTCACACACAGAAGCAGGTCCTCGACTATC
  TGGGCGAACGCTTCAGAGTAAAGCTCAGTCTTCCCGATTGGTACCCTAATGCGGAAGCTGCCGAGTTCCT
  GTTTAACCAGTGTATCTGCATCCACTTGAAATCCAACACTGACAAGTTTTACCTGCTCTGTCTCATGACC
  CGGAAGCTCTTTGCTTTAGCCAGAGGAGAGTGCATGGAGGACAATCCTGACAGTTTAGTGAATCAAGAAG
  TCCTTACCCCTGGGCAGCTCTTCCTGATGTTTCTGAAGGAAAAGATGGAGAATTGGCTACTGTCTATTAA
  AATAGCTTTAGATAAAAGGGCTCAGAAGACCAATGTTTCCATAAACAATGAAAATTTGATGAAGATTTTT
  AGTATGGGAACAGAGCTAACAAGACCATTTGAATATCTTCTTGCTACTGGAAATCTGCGTTCTAAAACAG
  GTCTTGGCTTCATGCAGGATTCTGGCCTGTGTGTTGTGGCTGACAAGCTGAACTTCATTCGCTATCTCTC
  CCATTTCCGCTGTGTGCACAGAGGGGCTGACTTTGCCAAGATGAGGACCACCACCGTGCGCAAGCTGCTG
  CCAGAATCCTGGGGCTTCCTCTGCCCTGTGCACACCCCAGACGGGGCACCGTGTGGGCTGCTGAACCACC
  TGACTGCTGTGTGTGAGGTTGTTACCAAGTTTGTGTACACAGCATCTATTCCAGCCTTGCTCTGTGGCTT
  AGGAGTCACTCCTGTTGATGCAGCACCATGTCGACCGTATAGTGACTGCTACCCTGTCCTGCTGGATGGC
  GTCATGGTGGGCTGGGTGGATAAGGAGCTGGCTCCTGAAGTGGCAGACACTCTCCGTCGATTTAAGGTGT
  TGAGAGAAAGGAGATGTTCCTCCCTGGATGGAGGTGGCCCTGATTCCCATGACAGGAAAGCCAAGCCTGT
  ACCCAGGGCTGTTCCTCTTCACCACTCCCTGCAGGCTGGTGAGGCCTGTGCAGAACCTGGAGCTGGGCAA
  AGAAGAGCTCGTTGGAACTATGGAGCAGCTCTTCATGAACATTGCCATCTTCGAGGACGAGGTTTTTGGT
  GGAGTTTCCACACACCAGGAGCTCTTCCCTCACAGCCTGCTGAGGTGATCGCCAACTTCATCCCCTTCTC
  TGATCACAACCAGAGTCCTCGGAACATGTACCAGTGCCAGATGGGTAAGCAGACCATGGGCTTCCCGCTG
  CTCACCTACCAAGACCGATCAGATAATAAACTCTATCGTCTCCAGACACCCCAGAGCCCTCTAGTGAGAC
  CGTGCATGTATGATCATTATGACATGGACAACTATCCCATCGGGACAAACGCCATTGTGGCTGTGATCTC
  CTACACTGGCTATGATATGGAGGACGCCATGATTGTAAACAAGGCCTCCTGGGAACGAGGCTTTGCTCAT
  GGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCTGAGAAATTTAAGCAAGGGGATGATAGTCTGGTAT
  TTGGGGTCAAACCTGGTGACCCACGGGTTATGCAGAAGCTGGACAATGATGGCTTGCCATTCATAGGAGC
  AAAGCTGGAGTTTGGTGATCCTTACTACGGCTACCTAAACCTTAACACCGGAGAAGGCTTCGTGGTTTAC
  TATAAGAGTAAAGAAAACTGTGTTGTGGACAACATCAAAGTGTGCAGTAATGACACAGGAAGTGGGAAGT
  TCAAGTGCGTCTGCGTCACCGTCCGAGTCCCCCGGAACCCAACTATTGGAGATAAGTTTGCCAGCCGTCA
  CGGACAGAAGGGCATTTTGAGCAGATTGTGGCCAGCTGAGGACATGCCTTTCACAGAGAGTGGGATGATG
  CCGGACATTCTGTTTAATCCTCATGGGTTTCCCTCCCGTATGACCATAGGTATGTTAATCGAGAGTATGG
  CTGGGAAGTCAGCAGCTTTGCATGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCCGAGGAGAACTC
  TGCCCTAGAGTACTTTGGTGAGATGTTAAAGGCTGCCGGCTACAACTTCTATGGCACGGAGAGATTGTAC
  AGCGGCATCAGCGGGATGGAGCTGGAGGCTGACATTTTCATTGGTGTGGTTTATTACCAGCGCCTACGAC
  ACATGGTGTCAGACAAATTTCAAGTCAGAACAACTGGAGCCAGGGACAAAGTCACCAACCAGCCCATTGG
  AGGCAGGAACGTCCAGGGTGGGATCCGATTTGGGGAGATGGAGCGGGATGCTCTGTTGGCGCACGGCACA
  TCTTTCCTTCTGCATGACCGCCTCTTCAACTGCTCCGACCGCTCTGTGGCCCACGTATGCGTGAAGTGTG
  GCAGTTTGCTTTCTCCGCTGCTCGAGAAGCCTCCCCCATCTTGGTCTGCGATGCGTAACAGAAAATACAA
  CTGCACCGTCTGCGGCCGCAGTGACTCCATCGACACTGTCTCTGTGCCGTATGTTTTCCGGTACTTTGTA
  GCTGAGCTGGCTGCCATGAACATCAAAGTGAAACTGGACGTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202655 representing NM_031773
  Red=Cloning site Green=Tags(s)
  
  MDVDSRWRNLPSGPSLKHLTDPSYAVPPEQQKAALQDLTRAHVDSFNYAVLEGLSHAVQAIPPFEFAFKD
  ERISLTIVDAAISPPAVPKGTICKELNIYPAECRGRRSTYRGKLTADISWAVNGVPKGIIKQFLGXVPIM
  VKSKLCNLYNLPPQVLIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKSRGLGYTQFGV
  SIHCVREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFKN
  SVSQMLRIVMEEGCHTQKQVLDYLGERFRVKLSLPDWYPNAEAAEFLFNQCICIHLKSNTDKFYLLCLMT
  RKLFALARGECMEDNPDSLVNQEVLTPGQLFLMFLKEKMENWLLSIKIALDKRAQKTNVSINNENLMKIF
  SMGTELTRPFEYLLATGNLRSKTGLGFMQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRKLL
  PESWGFLCPVHTPDGAPCGLLNHLTAVCEVVTKFVYTASIPALLCGLGVTPVDAAPCRPYSDCYPVLLDG
  VMVGWVDKELAPEVADTLRRFKVLRERRCSSLDGGGPDSHDRKAKPVPRAVPLHHSLQAGEACAEPGAGQ
  RRARWNYGAALHEHCHLRGRGFWWSFHTPGALPSQPAEVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPL
  LTYQDRSDNKLYRLQTPQSPLVRPCMYDHYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAH
  GSVYKSEFIDLSEKFKQGDDSLVFGVKPGDPRVMQKLDNDGLPFIGAKLEFGDPYYGYLNLNTGEGFVVY
  YKSKENCVVDNIKVCSNDTGSGKFKCVCVTVRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMM
  PDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLY
  SGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPIGGRNVQGGIRFGEMERDALLAHGT
  SFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDSIDTVSVPYVFRYFV
  AELAAMNIKVKLDVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031773
• ORF Size: 3405 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031773.1, NP_113961.1
• RefSeq Size: 3769
• RefSeq ORF: 3408
• Locus ID: 83582
• MW: 127.7 kDa
• Gene Summary: mouse homolog is the second largest subunit of RNA polymerase I [RGD, Feb 2006]