Ppp2cb (NM_017040) Rat Tagged ORF Clone

CAT#: RR202664

Ppp2cb (Myc-DDK-tagged ORF) - Rat protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (Ppp2cb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp2cb
• Synonyms: Pp2a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202664 representing NM_017040
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTGGAGCAGCTGAACGAGTGTAAGCAGCTGA
  ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCTAAGGAAATTTTAACAAAAGAATCAAATGTACAAGA
  GGTTCGCTGTCCTGTTACCGTCTGTGGAGATGTGCATGGCCAATTCCATGACCTTATGGAACTCTTCAGA
  ATTGGTGGAAAATCACCAGACACCAACTATCTATTCATGGGTGACTATGTAGACAGAGGATATTATTCTG
  TGGAGACCGTGACTCTTCTTGTAGCATTAAAGGTGCGCTATCCAGAGCGTATCACAATATTGCGAGGAAA
  TCATGAAAGCCGGCAGATCACACAAGTATATGGCTTTTATGATGAATGCCTACGAAAGTATGGGAACGCC
  AACGTGTGGAAATACTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT
  TCTGCCTCCACGGTGGCCTCTCTCCATCCATAGATACACTGGATCACATAAGAGCCCTGGATCGCTTACA
  GGAAGTTCCACATGAGGGCCCAATGTGTGATCTCTTATGGTCAGATCCAGATGACCGTGGTGGCTGGGGC
  ATTTCTCCACGTGGTGCTGGCTACACATTTGGACAAGACATTTCTGAAACATTTAACCATGCCAACGGCC
  TCACACTGGTGTCCCGTGCTCACCAGCTTGTAATGGAAGGATATAATTGGTGCCATGATCGGAATGTGGT
  CACCATTTTTAGTGCACCCAATTACTGCTACCGCTGTGGGAACCAGGCTGCTATCATGGAATTAGACGAC
  ACTTTAAAATACTCTTTTCTTCAGTTTGACCCAGCACCTCGTCGTGGAGAGCCTCATGTGACCCGGCGCA
  CCCCAGACTACTTCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202664 representing NM_017040
  Red=Cloning site Green=Tags(s)
  
  MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFR
  IGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNA
  NVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWG
  ISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDD
  TLKYSFLQFDPAPRRGEPHVTRRTPDYFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017040
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017040.1, NP_058736.1
• RefSeq Size: 1843
• RefSeq ORF: 930
• Locus ID: 24673
• MW: 35.6 kDa
• Gene Summary: catalytic subunit of multimeric serine/threonine phosphatase; involved in insulin signal transduction; negatively regulates the Ras/MAP kinase pathway by binding to Shc, inhibiting tyrosine phosphorylation [RGD, Feb 2006]