Rnaseh2b (NM_001007007) Rat Tagged ORF Clone

CAT#: RR202752

Rnaseh2b (Myc-DDK-tagged ORF) - Rat ribonuclease H2, subunit B (Rnaseh2b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnaseh2b
• Synonyms: MGC94780
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202752 representing NM_001007007
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGAGGTCGGGACCGCGGGGACTTGGCTGCTAGGCAGCTAGTGTTCCTACTTCCAGAATATTTAA
  AAGATGCCTCGAGGAAGTCGAAGAGCGGCCTCCTGTTTGTAAAGCTGGTCAACCCGCACTCAGGGGAAGG
  AGCCACTTACTTAATTGATGCGTGTCTACAGAAGCTGTTTGAAATAAAAGTTTTCAAGGAAAAACACCAT
  TCTTGGTTTATAAATCAGTCAGTTCAATCAGGAGGCCTTCTCCACTTTGCCACACCCATGGACCCTTTAT
  TTCTGCTCCTTCACTATCTCATAAAGGCTGGCAAAGAGGGGAAGTATCAGCCCTTGGATCAGGTTGTAGT
  GGATGACAAGTTTCCAGATTGCACCTTGCTGCTGAGATTTCCTGAGCTTGAGAAGTCACTTCGGCATGTG
  ACAGAGGAAAAAGAAGTGAACAGTAAGAAGTACTATAAGTACAGCACGGAGAAGACATTGAAGTGGCTGG
  AGAAGAAGGTCAACCAAACCGTGGCAGCACTGAAAGCCAATCATGTTAATGTTGGAGCCCGGGTACAGTC
  TTCTGCATATTTCTCTGGTGTTCAGGTTTCCAGGGACAAGGAAGAAGACTATGTTCGATATGCCCATGGT
  CTGATCTCTGATTACATCCCTAAAGAATTAAGTGACGATTTATCCAAGCTCTTGAAGCTTCCAGAACCTC
  CAGCCTCCCTGCCAAACCCTCCAGCAAAGAAACTAAAGTTAGCAGATGAGCCTGTGGAAGCGAAAGAAGA
  TTACACTAAGTTTAACACTAAGGACTTGAAGAACGGCAAGAAAAATAGCAAAATGACTGCAGCTCAGAAG
  GCTTTGGCTAAAGTTGACAAGAGCGGAATGAAAAGTATTGATGCTTTTTTTGGCGCAAAAAATAAAAAAA
  CTGGAAAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202752 representing NM_001007007
  Red=Cloning site Green=Tags(s)
  
  MAGGRDRGDLAARQLVFLLPEYLKDASRKSKSGLLFVKLVNPHSGEGATYLIDACLQKLFEIKVFKEKHH
  SWFINQSVQSGGLLHFATPMDPLFLLLHYLIKAGKEGKYQPLDQVVVDDKFPDCTLLLRFPELEKSLRHV
  TEEKEVNSKKYYKYSTEKTLKWLEKKVNQTVAALKANHVNVGARVQSSAYFSGVQVSRDKEEDYVRYAHG
  LISDYIPKELSDDLSKLLKLPEPPASLPNPPAKKLKLADEPVEAKEDYTKFNTKDLKNGKKNSKMTAAQK
  ALAKVDKSGMKSIDAFFGAKNKKTGKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007007
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007007.1, NP_001007008.1
• RefSeq Size: 1450 bp
• RefSeq ORF: 924 bp
• Locus ID: 361056
• Cytogenetics: 15p12
• MW: 34.6 kDa