Polr1a (NM_031772) Rat Tagged ORF Clone

CAT#: RR202782

Polr1a (Myc-DDK-tagged ORF) - Rat polymerase (RNA) I polypeptide A (Polr1a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polr1a
• Synonyms: Rpa1; Rpo1-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202782 representing NM_031772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCCTCCAAGCACACGCCATGGCGGCGGCTTCAGGGCATTTCCTTCGGGATGTATTCGGCCGAGG
  AGCTCAAGAAGTTAAGTGTTAAGTCCATTACAAATCCTCGCTATGTGGACAGCCTGGGAAACCCTTCGGC
  AGATGGCCTGTATGATTTGGCTTTGGGTCCTGCAGATTCCAAAGAAGTATGCTCTACCTGTGTGCAGGAC
  TTCAACAACTGCTCCGGACACCTGGGCCACATTGACCTCCCGCTCACAGTGTACAACCCTCTCCTCTTCG
  ATAAACTCTACCTGCTGCTGCGAGGCTCCTGTTTAAATTGTCATATGCTGACTTGTCCTCGGGCTGCAAT
  TCACCTATTGGTCTGCCAGCTGAAGGTCCTAGACGTTGGAGCCCTACAAGCAGTCTATGAGCTTGAGCGG
  ATTCTCAGCAGGTTTCTGGAAGAAACTTCGGATCCTTCTGCCTTCGAAATTCAAGAAGAGTTAGAAGAGT
  ACACAAGCAAAATTCTGCAGAATAACCTCCTGGGGTCCCAGGGTGCACATGTGAAAAATGTGTGCGAGAG
  CAGAAGCAAACTTGTCGCTCACTTCTGGAAGACACACATGGCTGCTAAGCGCTGTCCACACTGCAAGACG
  GGACGGTCAGTGGTTCGAAAGGAGCACAATAGTAAGCTAACAATAACATATCCAGCCATGGTGCACAAGA
  AGTCTGGCCAGAAGGATGCAGAACTCCCAGAAGGAGCACCAGCAGCTCCAGGAATTGATGAGGCTCAGAT
  GGGGAAACGAGGATACTTGACCCCAAGCAGCGCCCAAGAACATCTTTTCGCCATTTGGAAGAATGAAGGA
  TTCTTCCTGAATTATCTTTTTTCGGGATTGGATGATATTGGCCCAGAGTCCAGTTTCAACCCCAGTATGT
  TCTTTCTAGATTTCATCGTGGTACCGCCATCCAGGTATCGTCCCATCAATCGCCTGGGTGACCAGATGTT
  CACTAATGGCCAGACAGTGAATTTGCAAGCGGTCATGAAGGATGCAGTTTTGATCCGGAAACTTCTGGCA
  GTGATGGCCCAAGAACAGAAACTGCCCTGTGAGATGACAGAAATCACCATAGACAAGGAAAATGACTCCT
  CGGGTGCTATCGACCGGTCCTTTCTGAGTTTGCTTCCAGGACAGTCTCTCACAGATAAACTTTACAATAT
  TTGGATTCGCCTCCAGAGCCACGTCAATATTGTGTTTGACAGTGACATGGACAAACTGATGCTGGAAAAG
  TACCCTGGCATAAGACAGATCCTCGAGAAGAAGGAAGGCCTGTTCCGGAAGCACATGATGGGAAAGCGAG
  TGGACTACGCAGCCCGCTCGGTCATCTGCCCAGATATGTACATCAACACCAATGAGATCGGGATTCCCAT
  GGTATTTGCCACAAAACTGACTTACCCCCAGCCAGTTACCCCCTGGAACGTGCAGGAACTGAGACAGGCA
  GTCATCAATGGACCCAATGTGCACCCAGGAGCCTCCATGGTCATCAACGAGGATGGCAGTCGCACAGCCC
  TGAGCGCAGTGGATGCTACACAGCGGGAAGCTGTGGCCAAACAGCTCCTGACACCGTCCACAGGGATCCC
  TAAGCCTCAGGGAGCAAAAGTTGTGTGCCGGCATGTGAAGAATGGGGACATTCTCCTCCTGAACCGACAG
  CCCACCTTGCACAGACCCTCCATCCAGGCCCACCGTGCCCACATTCTGCCTGAGGAAAAAGTCCTGCGCC
  TTCACTATGCCAACTGCAAGGCCTACAATGCTGACTTCGATGGAGACGAGATGAATGCCCACTTCCCCCA
  GAGTGAGCTGGGCCGAGCTGAAGCCTATGTCCTGGCCTGCACTGACCAGCAGTATCTTGTTCCCAAGGAT
  GGCCAGCCCTTGGCAGGACTGATTCAGGATCACATGGTGTCGGGTGCAAACATGACCATTCGGGGATGCT
  TTTTCACCCGGGAACAGTACATGGAGCTGGTGTACCGAGGGCTCACAGACAAAGTGGGACGCGTGAAGCT
  CTTTCCTCCTGCCATCCTGAAGCCCTTCCCGCTGTGGACAGGAAAGCAGGTCGTGTCGACCCTCCTCATA
  AACATAATCCCAGAGGACTACACCCCCCTGAATTTAACTGGAAAGGCAAAAATCGGCAGTAAAGCCTGGG
  TGAAGGAAAAGCCTCGGCCTGTTCCTGACTTTGACCCTGACTCCATGTGTGAGTCCCAGGTGATCATCAG
  GGAAGGGGAGCTGCTCTGCGGGGTGCTGGACAAGGCACACTATGGGAGCTCTGCCTACGGCCTGGTCCAC
  TGCTGCTACGAGATCTATGGGGGTGAGACCAGCGGCAGGGTTCTCACCTGCTTAGCCCGCCTCTTCACTG
  CCTACCTACAGCTCTATCGAGGTTTCACCTTGGGTGTGGAAGACATTTTGGTTAAGCCAAATGCAGATGT
  GATGAGACAGCGCATCATTGAAGAATCTACCCAGTGTGGACCCCGGGCTGTCAGGGCTGCGTTGAACCTG
  CCAGAGGCTGCATCATGTGATGAGATCCAGGGGAAATGGCAGGATGCCATCTGGCGCAAAGACCAGAGGG
  ATTTTAACATGATTGATATGAAGTTCAAGGAGGAAGTGAACCATTACAGCAATGAGATTAACAAGGCATG
  TATGCCTTTTGGCTTGCACAGACAATTCCCTGAAAACAACCTGCAGATGATGGTGCAGTCGGGAGCCAAA
  GGGTCGACCGTGAACACGATGCAAATCTCTTGCCTGCTGGGCCAAATTGAGTTGGAAGGTCGGAGACCCC
  CACTGATGGCGTCTGGCAAGTCACTGCCCTGCTTTGAGCCTTACGAGTTCACCCCAAGGGCTGGTGGCTT
  TGTCACTGGAAGGTTCCTTACCGGTATCAGGCCACCTGAGTTCTTCTTCCACTGCATGGCGGGGCGAGAA
  GGCCTGGTGGATACTGCTGTCAAGACCAGCCGTTCTGGCTATCTCCAAAGGTGTATCATCAAGCACCTGG
  AGGGTCTGGTCATCCAGTACGATCTCACGGTCCGTGACAGTGATGGCAGTGTGGTACAGTTCCTGTATGG
  GGAGGATGGCCTGGACATCCCCAAGACACAGTTCCTACAACCCAAGCAGTTCCCCTTTCTGGCCAGCAAT
  TATGAGGTGATAATGAAATCCAAGCATCTTCATGAAGTTTTGTCTAGAGCAGATCCCCAGAAAGTGCTCC
  GCCACTTCAGAGCCATTAAAAAATGGCACCACAGGCATTCCAGTGCCCTGCTGAGAAAAGGCGCCTTCCT
  GAGTTTTTCCCAGAAGATTCAGGCAGCTGTGAAAGCCTTGAACCTAGAGGGCAAGACCCAGAATGGGCGC
  AGCCCAGAGACCCAGCAGATGTTACAGATGTGGCATGAATTAGATGAGCAGAGCCGACGGAAATACCAGA
  AGAGGGCTGCCCCGTGTCCCGACCCTAGCCTTTCTGTGTGGCGCCCAGACATCCACTTCGCATCTGTGTC
  TGAAACATTTGAAAAAAAGATTGATGACTACAGTCAGGAGTGGGCAGCTCAAGCAGAGAAGAGTCACAAC
  AGATCAGAGCTTTCTCTTGACAGGCTGAGAACCTTACTGCAGCTCAAGTGGCAGCGCTCACTGTGTGACC
  CAGGCGAGGCTGTTGGCCTCCTGGCCGCCCAGAGCATCGGAGAGCCCTCCACACAGATGACACTGAACAC
  CTTCCACTTTGCAGGCAGAGGCGAGATGAACGTCACCCTGGGCATTCCAAGATTGAGGGAGATTCTCATG
  GTGGCCAGTGCCAACATCAAAACACCCATGATGAGCGTGCCTGTGTTCAACACCAAGAAAGCACTGAGGA
  GAGTGAAGAGTCTGAAGAAGCAGCTCACCAGGGTGTGCCTGGGGGAGGTATTGCAGAAAGTTGACATCCA
  GGAGTCCTTCTGTATGGGAGAGAAACAGAATAAATTCCGGGTGTATGAGCTCCGGTTTCAGTTCCTGCCA
  CATGCATATTACCAGCAGGAGAAGTGCCTGAGACCCGAGGACATCTTGCACTTCATGGAAACCAGATTCT
  TTAAGCTACTAATGGAAGCTATCAAAAAGAAGAATAGCAAAGCATCAGCCTTCAGAAGTGTGAATACCAG
  GAGAGCTACACAGAAGGACCTCGATGACACTGAGGACTCGGGGAGGAATCGGAGAGAGGAGGAAAGGGAT
  GAGGAAGAGGAGGGGAACATTGTGGATGCTGAAGCTGAGGAGGGGGATGCAGATGCTTCTGACACCAAAC
  GCAAGGAGAAGCAAGAAGAAGAGGTTGACTATGAGAGTGAGGAAGAAGGGGAGGAGGAAGAGGAAGAGGA
  TGTACAAGAAGAGGAGAACATCAAAGGTGAAGGTGCCCACCAGACCCATGAGCCAGATGAGGAAGAAGGT
  TCAGGCCTTGAGGAGGAATCATCCCAGAACCCTCCCTGCCGCCATTCCAGGCCTCAGGGAGCTGAGGCCA
  TGGAACGCCGAATCCAGGCTGTACGGGAGTCCCATTCGTTCATTGAGGACTACCAGTATGACACAGAGGA
  GAGCCTGTGGTGCCAGGTGACAGTGAAGCTCCCATTGATGAAGATTAATTTTGACATGAGCTCCTTGGTT
  GTATCCTTGGCCCACAATGCCATTGTCTATACGACTAAGGGCATCACTCGGTGTCTCCTGAACGAAACGA
  TCAACAGTAAGAATGAGAAGGAGTTTGTCCTGAACACGGAGGGAATCAACCTCCCCGAGCTGTTCAAGTA
  CTCCGAGGTGCTGGACCTGCGCCGCCTGTACTCCAACGACATCCATGCTGTGGCCAATACGTACGGCATC
  GAGGCTGCGCTGCGGGTGATAGAGAAGGAGATTAAGGATGTGTTTGCTGTGTACGGCATTGCGGTTGATC
  CTCGCCATCTCTCCCTGGTTGCTGATTATATGTGTTTTGAGGGTGTTTATAAGCCACTCAATCGCTTTGG
  GATCCAGTCAAGCTCCTCCCCTTTGCAGCAGATGACGTTTGAAACCAGCTTCCAGTTTCTGAAACAGGCC
  ACCATGATGGGATCCCATGATGAACTGAAGTCACCTTCTGCCTGCCTTGTGGTGGGAAAGGTCGTCAAAG
  GCGGGACAGGCCTGTTTGAGCTCAAGCAGCCCCTAAGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202782 representing NM_031772
  Red=Cloning site Green=Tags(s)
  
  MLASKHTPWRRLQGISFGMYSAEELKKLSVKSITNPRYVDSLGNPSADGLYDLALGPADSKEVCSTCVQD
  FNNCSGHLGHIDLPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAAIHLLVCQLKVLDVGALQAVYELER
  ILSRFLEETSDPSAFEIQEELEEYTSKILQNNLLGSQGAHVKNVCESRSKLVAHFWKTHMAAKRCPHCKT
  GRSVVRKEHNSKLTITYPAMVHKKSGQKDAELPEGAPAAPGIDEAQMGKRGYLTPSSAQEHLFAIWKNEG
  FFLNYLFSGLDDIGPESSFNPSMFFLDFIVVPPSRYRPINRLGDQMFTNGQTVNLQAVMKDAVLIRKLLA
  VMAQEQKLPCEMTEITIDKENDSSGAIDRSFLSLLPGQSLTDKLYNIWIRLQSHVNIVFDSDMDKLMLEK
  YPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQA
  VINGPNVHPGASMVINEDGSRTALSAVDATQREAVAKQLLTPSTGIPKPQGAKVVCRHVKNGDILLLNRQ
  PTLHRPSIQAHRAHILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKD
  GQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLI
  NIIPEDYTPLNLTGKAKIGSKAWVKEKPRPVPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVH
  CCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVMRQRIIEESTQCGPRAVRAALNL
  PEAASCDEIQGKWQDAIWRKDQRDFNMIDMKFKEEVNHYSNEINKACMPFGLHRQFPENNLQMMVQSGAK
  GSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGRE
  GLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASN
  YEVIMKSKHLHEVLSRADPQKVLRHFRAIKKWHHRHSSALLRKGAFLSFSQKIQAAVKALNLEGKTQNGR
  SPETQQMLQMWHELDEQSRRKYQKRAAPCPDPSLSVWRPDIHFASVSETFEKKIDDYSQEWAAQAEKSHN
  RSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILM
  VASANIKTPMMSVPVFNTKKALRRVKSLKKQLTRVCLGEVLQKVDIQESFCMGEKQNKFRVYELRFQFLP
  HAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKNSKASAFRSVNTRRATQKDLDDTEDSGRNRREEERD
  EEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEDVQEEENIKGEGAHQTHEPDEEEG
  SGLEEESSQNPPCRHSRPQGAEAMERRIQAVRESHSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSLV
  VSLAHNAIVYTTKGITRCLLNETINSKNEKEFVLNTEGINLPELFKYSEVLDLRRLYSNDIHAVANTYGI
  EAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQA
  TMMGSHDELKSPSACLVVGKVVKGGTGLFELKQPLR
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031772
• ORF Size: 5148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031772.1, NP_113960.1
• RefSeq Size: 6018
• RefSeq ORF: 5151
• Locus ID: 83581
• MW: 194.2 kDa
• Gene Summary: subunit of RNA polymerase I [RGD, Feb 2006]