Pck1 (NM_198780) Rat Tagged ORF Clone

CAT#: RR202791

Pck1 (Myc-DDK-tagged ORF) - Rat phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pck1
• Synonyms: GTP; PCK; Pepck; PEPCK-C; RATPEPCK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202791 representing NM_198780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCTCAGCTGCATAATGGTCTGGACTTCTCTGCCAAGGTCATCCAGGGCAGCCTCGACAGCCTGC
  CCCAGGAAGTGAGGAAGTTTGTGGAAGGCAATGCCCAGCTGTGCCAGCCAGAGTATATTCACATCTGCGA
  CGGCTCCGAGGAGGAGTACGGGCGGCTGCTGGCCCACATGCAGGAGGAGGGTGTCATCCGCAAGCTGAAG
  AAATATGACAACTGTTGGCTGGCTCTCACTGACCCCAGGGATGTGGCCAGGATCGAAAGCAAGACGGTCA
  TCATTACCCAAGAGCAGAGAGACACCGTGCCCATCCCCAAAAGTGGGCAGAGCCAGCTGGGCCGCTGGAT
  GTCAGAAGAGGACTTCGAGAAAGCATTCAACGCCAGGTTCCCGGGGTGCATGAAAGGCCGCACCATGTAT
  GTCATCCCATTCAGCATGGGGCCGCTGGGCTCACCTCTGGCCAAGATTGGTATTGAGCTGACAGACTCGC
  CCTATGTGGTGGCCAGCATGCGGATCATGACACGGATGGGGACCTCTGTGCTGGAGGCCCTGGGCGATGG
  GGAGTTTATCAAGTGCCTCCACTCCGTGGGGTGCCCTCTCCCCTTAAAAAAGCCTTTGGTCAACAACTGG
  GCCTGCAACCCCGAGCTGACCCTGATTGCTCACCTCCCGGACCGCAGAGAGATCATCTCCTTCGGAAGCG
  GATACGGTGGGAACTCACTGCTTGGGAAGAAATGCTTTGCGCTGCGGATCGCCAGCAGGCTGGCTAAGGA
  GGAAGGGTGGCTGGCGGAGCACATGCTGATCCTGGGCATAACTAACCCCGAAGGCAAGAAGAAATACCTG
  GCAGCAGCCTTCCCCAGTGCCTGTGGGAAAACCAACCTGGCCATGATGAACCCCACCCTCCCCGGGTGGA
  AAGTTGAATGTGTGGGTGATGACATTGCCTGGATGAAGTTTGATGCCCAAGGCAACTTAAGGGCCATCAA
  CCCAGAAAACGGTTTTTTTGGAGTTGCTCCGGGCACCTCAGTGAAGACAAATCCGAACGCCATTAAGACC
  ATCCAGAAAAACACCATCTTCACCAACGTGGCTGAGACAAGTGATGGGGGTGTTTACTGGGAAGGCATCG
  ATGAGCCCCTGGCCCCAGGAGTCACCATCACTTCCTGGAAGAACAAAGAGTGGAGACCACAGGATGAGGA
  ACCGTGCGCCCATCCCAACTCGCGATTCTGCACCCCTGCCAGCCAATGTCCCATTATTGACCCCGCCTGG
  GAATCTCCTGAAGGAGTGCCCATCGAAGGCATCATTTTTGGTGGCCGTAGACCTGCAGGTGTCCCCCTTG
  TCTACGAAGCTCTCAGCTGGCAGCATGGGGTGTTTGTAGGAGCTGCCATGAGATCAGAGGCCACCGCTGC
  TGCAGAGCATAAGGGCAAGGTCATCATGCACGACCCCTTCGCTATGCGGCCCTTCTTTGGCTACAACTTC
  GGCAAGTACCTGGCGCACTGGCTGAGCATGGCCCACCGCCCAGCAGCCAAGTTGCCCAAGATCTTCCACG
  TCAACTGGTTCCGGAAAGACAAAAACGGCAAGTTCCTCTGGCCCGGATTTGGTGAGAACTCCCGCGTGCT
  GGAGTGGATGTTCGGACGCATCGAAGGGGAAGACAGCGCCAAGCTCACTCCCATTGGCTACGTCCCTAAG
  GAAGACGCCCTGAACTTGAAAGGCCTGGGGGACGTCAACGTGGAGGAGCTGTTCGGAATCTCTAAGGAAT
  TCTGGGAGAAGGAGGTGGAGGAGATCGACAAGTATCTGGAGGACCAGGTCAACGCCGACCTCCCTTACGA
  AATAGAGAGGGAGCTCCGAGCCCTGAAACAGAGAATCAGCCAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202791 representing NM_198780
  Red=Cloning site Green=Tags(s)
  
  MPPQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLK
  KYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMY
  VIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNW
  ACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYL
  AAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKT
  IQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAW
  ESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNF
  GKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPK
  EDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198780
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_198780.1, NM_198780.2, NM_198780.3, NP_942075.1
• RefSeq Size: 2644
• RefSeq ORF: 1869
• Locus ID: 362282
• MW: 69.4 kDa
• Gene Summary: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. [UniProtKB/Swiss-Prot Function]