Gpt (NM_031039) Rat Tagged ORF Clone

CAT#: RR202833

Gpt (Myc-DDK-tagged ORF) - Rat glutamic-pyruvate transaminase (alanine aminotransferase) (Gpt), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpt
• Synonyms: ALAT; Gpt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202833 representing NM_031039
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACGGGTGAATGATCAAAGCCAGGCTTCAAGGAATGGGCTGAAGGGAAAGGTGCTAACTCTGG
  ACACTATGAACCCATGTGTGCGGAGGGTGGAGTATGCAGTTCGAGGACCCATTGTGCAGCGTGCCTTGGA
  GCTGGAGCAGGAGCTGCGTCAGGGTGTGAAGAAGCCGTTTACTGAGGTCATCCGTGCCAACATTGGGGAT
  GCACAAGCCATGGGGCAGAGACCCATCACCTTCTTCCGCCAGGTCCTGGCCCTCTGTGTCTACCCCAATC
  TTCTGAGCAGTCCTGACTTCCCAGAGGATGCCAAGAGAAGGGCAGAACGCATCTTGCAGGCCTGCGGGGG
  CCACAGCCTGGGTGCCTATAGCATTAGCTCTGGAATCCAGCCGATCCGGGAGGATGTGGCGCAATACATT
  GAGAGAAGAGACGGAGGCATCCCCGCAGACCCGAACAACATATTTCTATCCACAGGGGCCAGCGATGCCA
  TCGTGACAATGCTCAAGCTGCTGGTATCTGGCGAGGGCCGTGCACGAACAGGTGTACTCATTCCCATTCC
  TCAGTACCCACTGTACTCAGCCGCGCTGGCTGAACTGGACGCCGTGCAAGTGGACTACTACCTGGACGAA
  GAGCGCGCCTGGGCTCTGGACATCGCAGAGCTGCGGCGCGCTCTGTGCCAGGCACGTGACCGTTGCTGCC
  CTCGAGTACTGTGCGTCATCAACCCCGGCAACCCCACTGGGCAGGTGCAGACCCGTGAGTGCATCGAGGC
  CGTAATCCGCTTTGCTTTCAAAGAAGGACTCTTCTTGATGGCTGATGAGGTATACCAGGACAACGTGTAT
  GCCGAGGGCTCTCAGTTCCATTCATTCAAGAAGGTGCTCATGGAGATGGGGCCACCGTATTCCACGCAGC
  AGGAGCTTGCTTCTTTCCACTCAGTCTCTAAGGGCTACATGGGCGAGTGCGGGTTTCGTGGTGGCTATGT
  GGAGGTGGTAAACATGGATGCTGAGGTGCAGAAACAGATGGGGAAGCTGATGAGTGTGCGGCTGTGTCCA
  CCAGTGCCAGGCCAGGCCTTGATGGACATGGTGGTCAGTCCGCCAACACCCTCCGAGCCGTCCTTCAAGC
  AGTTTCAAGCAGAGAGACAGGAGGTGCTGGCTGAACTGGCAGCCAAGGCTAAGCTCACGGAGCAGGTCTT
  CAATGAGGCTCCCGGGATCCGCTGCAACCCAGTGCAGGGCGCCATGTATTCCTTCCCTCAAGTGCAGCTG
  CCCTTGAAAGCGGTGCAGCGTGCTCAGGAACTGGGCCTGGCCCCTGACATGTTCTTCTGCCTGTGCCTCC
  TGGAAGAGACTGGCATCTGCGTTGTGCCCGGGAGTGGCTTTGGGCAGCAGGAGGGCACCTATCATTTCCG
  GATGACCATTCTGCCCCCCATGGAGAAACTGCGGCTGCTGCTGGAAAAACTCAGTCACTTCCATGCCAAG
  TTCACCCATGAGTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202833 representing NM_031039
  Red=Cloning site Green=Tags(s)
  
  MASRVNDQSQASRNGLKGKVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGD
  AQAMGQRPITFFRQVLALCVYPNLLSSPDFPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYI
  ERRDGGIPADPNNIFLSTGASDAIVTMLKLLVSGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDE
  ERAWALDIAELRRALCQARDRCCPRVLCVINPGNPTGQVQTRECIEAVIRFAFKEGLFLMADEVYQDNVY
  AEGSQFHSFKKVLMEMGPPYSTQQELASFHSVSKGYMGECGFRGGYVEVVNMDAEVQKQMGKLMSVRLCP
  PVPGQALMDMVVSPPTPSEPSFKQFQAERQEVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPQVQL
  PLKAVQRAQELGLAPDMFFCLCLLEETGICVVPGSGFGQQEGTYHFRMTILPPMEKLRLLLEKLSHFHAK
  FTHEYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031039
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031039.1, NP_112301.1
• RefSeq Size: 1744
• RefSeq ORF: 1491
• Locus ID: 81670
• MW: 55.1 kDa
• Gene Summary: human homolog is a cytosolic alanine aminotransferase [RGD, Feb 2006]