Pcdhga1 (NM_001037140) Rat Tagged ORF Clone

CAT#: RR202877

Pcdhga1 (Myc-DDK-tagged ORF) - Rat protocadherin gamma subfamily A, 1 (Pcdhga1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pcdhga1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202877 representing NM_001037140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATTCCAGAGAAGCTAACCGGTTGGAGCCTGCTGATGCTGCTGTGCCTTTCTCTGGAGCTACTGT
  TGGAAGCTGGGGCTGGAAACATCCGATACTCCGTGCCAGAAGAAACCGACAAAGGCTCCTTCGTGGGCAG
  CATCGCCAAGGACCTGGGACTGGAAACCCGGGAGCTGGCGGAGCGCAGGATCCGCATCGTCTCCAGAGGT
  AGGTCGCAGCTTTTCTCCCTGAACCCGCGAAGCGGCAGCTTGGTCACCGCGGGCAGGATAGACCGGGAAG
  AGCTGTGCGCTCAGAGCGTGCCGTGTGTCGTGAGCTTTAACATCCTTATGGAGGATAAAGTAAAGCTTGT
  CCCTATCGAAGTAGAGATAATTGATATCAATGACAACACTCCCCAATTCCAGTTAGAAGAGCTAGAATTA
  AAAATGAGTGAAATAACGACCCCAGGTACCAGGATCCCTTTGCCTCTCGGGCAAGACCTGGATGTGGGTA
  TAAATTCACTTCAGAGCTACCAGCTAAGCGCCAACCCTCACTTCTTCCTGGATGTGCAACAGGGACCTGA
  TGGACCACAACAGCCGGAGATGGTACTGCAGAGACCTCTAGACAGAGAAAAAGATGCTGTTCATTACCTG
  ATCCTCACTGCCTCTGATGGGGGCAACCCAATACATTCAGGGACTTTGCAAATTCATGTTCAGGTGGTAG
  ATGTGAACGACAACCCACCAGCATTTACTCAGGCAGAGTATCACGTGAGAGTCCCTGAGAACGTTCCATT
  AGGCACTCGGCTTCTCAAGGTGAATGCCTCTGACCCGGATGAGGGAGCTAATGGCAGAGTAACCTACTCC
  TTTCACAATGTAGACCACAGCGTGGTCCGGAAATTTCAGTTGGATTCTTACACAGGAGAACTATCGAATA
  AAGAACCACTAGACTTTGAAGAATACAAAGTTTATCCAATGGAAATTCAAGCTCAGGATGGTGCTGGACT
  CATGGCCAGAGCGAAGGTGCTGGTCACTGTTTTGGATGTTAATGATAATGCCCCAGAAATTGGAGTCACG
  TCTGTAACTACCACAATACCAGAAAACTTTCCTCCTGGGACCATAGTTGCTCTCATCAGTGTGCATGATC
  AAGACGCTGATGACAATGGTCACACCACTTGTTCCATTCCTGGCAACCTACCCTTTAAACTGGAAAAGTT
  AGTGGACAATTATTACCGCTTAGTAACAGAAAGAACACTGGACAGAGAACATAGCTCTAGGTACAACATC
  ACAATAACAGCAACAGATCAGGGAACTCCACCCCTATCTACCCAGGCTCACATCTCACTGCTAGTGACAG
  ATATCAATGACAACCCTCCAGTTTTCGAACAGGACTCCTATTCTGTCTACATCCCTGAAAACAACCCCAG
  AGGGGCTTCCATCTTCTCAGTAAAGGCCCAGGATGCTGACCACAATGAGAATGCTCTAGTGACTTACGCC
  CTGGTGGAAGACACTATTCAGGGAGTACCTTTGTCTTCTTATTTCTCCATCAACTCTGACACTGGAGTTG
  TCTATGCACTGCAATCCTTTGACTATGAGCAGTTTCGAGATTTGCAGCTAAGAGTGATGGCCCGTGATAG
  TGGGAACCCACAACTCAGCAGCAATGTATCTCTGAGTCTATTCATGATAGATCAGAATGACAACGCCCCA
  GAGATCCTGTACCCTGTCCTCCCCACTGATGGCTCCACTGGTGTGGAGCTAGCACCTCGATCTGCCGAGC
  CCGGTTACCTGGTGACCAAGGTAGTAGCAGTAGACAAAGACTCAGGACAGAACTCCTGGCTGTCCTACCG
  TCTGCTCAAAGCCAGCGAGCCAGGGCTCTTCTCGGTGGGGCTGCACACAGGTGAGGTACGCACAGCTCGG
  GCCCTGCTGGACAGAGATGCTCTCAAACAGAGCCTGGTGGTGGCTGTGCAGGACCACGGCCAGCCTCCTC
  TGTCCACCACAGTCACATTTACTATAGCAGTGGCTGACAACATCCCTGACCTCCTGGCTGACCTGGGCAG
  CATTGGGACCCCTGCTGACCCCCAGGATTCAGATCTCACACTCTACCTGGTGGTGGCAGTGGCCGTAGTC
  TCCTGCATCTTCCTAGCCTTTGTAATAGTGCTCTTGGTGCTCAAGCTGCGACATTGGCACTCCTCACGCT
  TGCTCCAGACTGCAGCAAATGGACTGACTGACCTGCCCACCTCCAACTTTGTAGGTGTTGATGGGGTACA
  TGCATTTCTACAGACCTATTCCCATGAGATCTCACTCACTGCTGACTCTCGTAAGAGCCACTTGATCTTC
  CCGCAACCCAACTACGCAGACACACTCATCAGCCAAGAGAGCTGTGAGAAAAAAGAATTTCTGTCAGCAC
  CACAGTCTCTGCTTGAAGATAAAGAGGAAATATTTTCTCAGCAAGCCCCGCCCAACACTGACTGGCGTTT
  CTCTCAAGCCCAGAGACCCGGCACGAGCGGATCCCAAAATGGGGATGAAACCGGCACCTGGCCCAACAAC
  CAGTTCGATACAGAGATGCTGCAAGCCATGATCTTGGCCTCTGCCAGTGAAGCCGCTGATGGGAGCTCCA
  CTCTGGGAGGGGGCACTGGCACTATGGGTTTGAGCGCTCGATATGGACCCCAGTTTACCCTGCAGCACGT
  GCCTGACTACCGCCAGAACGTGTACATCCCTGGCAGCAATGCCACACTGACCAACGCAGCTGGCAAACGA
  GATGGCAAGGCTCCGGCAGGCGGCAATGGCAACAAGAAGAAGTCGGGCAAGAAAGAGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202877 representing NM_001037140
  Red=Cloning site Green=Tags(s)
  
  MAIPEKLTGWSLLMLLCLSLELLLEAGAGNIRYSVPEETDKGSFVGSIAKDLGLETRELAERRIRIVSRG
  RSQLFSLNPRSGSLVTAGRIDREELCAQSVPCVVSFNILMEDKVKLVPIEVEIIDINDNTPQFQLEELEL
  KMSEITTPGTRIPLPLGQDLDVGINSLQSYQLSANPHFFLDVQQGPDGPQQPEMVLQRPLDREKDAVHYL
  ILTASDGGNPIHSGTLQIHVQVVDVNDNPPAFTQAEYHVRVPENVPLGTRLLKVNASDPDEGANGRVTYS
  FHNVDHSVVRKFQLDSYTGELSNKEPLDFEEYKVYPMEIQAQDGAGLMARAKVLVTVLDVNDNAPEIGVT
  SVTTTIPENFPPGTIVALISVHDQDADDNGHTTCSIPGNLPFKLEKLVDNYYRLVTERTLDREHSSRYNI
  TITATDQGTPPLSTQAHISLLVTDINDNPPVFEQDSYSVYIPENNPRGASIFSVKAQDADHNENALVTYA
  LVEDTIQGVPLSSYFSINSDTGVVYALQSFDYEQFRDLQLRVMARDSGNPQLSSNVSLSLFMIDQNDNAP
  EILYPVLPTDGSTGVELAPRSAEPGYLVTKVVAVDKDSGQNSWLSYRLLKASEPGLFSVGLHTGEVRTAR
  ALLDRDALKQSLVVAVQDHGQPPLSTTVTFTIAVADNIPDLLADLGSIGTPADPQDSDLTLYLVVAVAVV
  SCIFLAFVIVLLVLKLRHWHSSRLLQTAANGLTDLPTSNFVGVDGVHAFLQTYSHEISLTADSRKSHLIF
  PQPNYADTLISQESCEKKEFLSAPQSLLEDKEEIFSQQAPPNTDWRFSQAQRPGTSGSQNGDETGTWPNN
  QFDTEMLQAMILASASEAADGSSTLGGGTGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKR
  DGKAPAGGNGNKKKSGKKEKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037140
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037140.1, NP_001032217.1
• RefSeq Size: 4548 bp
• RefSeq ORF: 2796 bp
• Locus ID: 553129
• Cytogenetics: 18p11
• MW: 101.6 kDa