Eef2 (NM_017245) Rat Tagged ORF Clone

CAT#: RR202878

Eef2 (Myc-DDK-tagged ORF) - Rat eukaryotic translation elongation factor 2 (Eef2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eef2
• Synonyms: Ef-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202878 representing NM_017245
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAACTTCACAGTAGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCCGGAACATGTCAG
  TCATCGCTCACGTGGACCACGGCAAGTCCACGTTGACCGACTCCCTTGTGTGCAAGGCTGGCATCATCGC
  CTCTGCCCGAGCCGGGGAGACACGCTTCACTGACACTCGAAAGGATGAGCAGGAGCGCTGCATCACCATC
  AAGTCCACTGCCATCTCCCTCTTCTATGAGCTCTCCGAGAACGACCTGAACTTCATTAAGCAGAGCAAGG
  ATGGCTCGGGCTTCCTCATCAACCTCATTGACTCTCCAGGTCATGTGGACTTCTCCTCAGAGGTGACCGC
  TGCTCTGCGTGTCACTGATGGAGCACTGGTGGTGGTGGACTGTGTGTCTGGTGTCTGTGTGCAGACAGAG
  ACAGTGCTGCGGCAGGCCATCGCTGAGCGCATCAAGCCCGTGCTGATGATGAACAAGATGGACCGGGCCC
  TGCTGGAACTGCAACTGGAGCCTGAGGAGCTCTACCAGACCTTCCAGCGCATCGTGGAGAACGTCAATGT
  CATCATCTCCACCTATGGCGAGGGCGAGAGTGGACCCATGGGCAATATCATGATCGATCCTGTCCTGGGC
  ACTGTAGGCTTTGGCTCTGGCCTGCATGGGTGGGCCTTCACACTGAAGCAGTTTGCAGAGATGTATGTGG
  CCAAGTTTGCAGCCAAGGGTGAGGGCCAGCTGGGTGCAGCTGAGCGGGCCAAGAAGGTAGAAGACATGAT
  GAAGAAGCTGTGGGGAGACCGGTACTTTGACCCGGCCAACGGCAAGTTCAGCAAGTCAGCCAATAGCCCA
  GACGGGAAGAAACTTCCCCGCACCTTCTGCCAGCTCATCTTGGACCCCATCTTCAAGGTGTTTGATGCCA
  TCATGAACTTCAGGAAGGAGGAGACAGCCAAGCTGATCGAGAAGCTGGACATCAAGCTGGACAGTGAGGA
  CAAGGACAAGGAGGGCAAACCACTGCTGAAGGCTGTGATGCGTCGCTGGCTGCCTGCTGGTGACGCCCTG
  CTACAGATGATCACCATCCACTTGCCGTCCCCGGTCACTGCACAGAAGTACCGTTGTGAGCTGCTGTACG
  AGGGCCCACCTGATGACGAGGCCGCCATGGGTATTAAGAGCTGCGACCCCAAAGGCCCCCTAATGATGTA
  CATCTCCAAGATGGTGCCAACATCTGACAAAGGCCGCTTCTATGCCTTCGGTAGAGTGTTCTCTGGGGTG
  GTGTCCACAGGTCTGAAGGTCCGGATCATGGGCCCCAACTATACACCTGGGAAGAAGGAGGACCTGTACC
  TGAAGCCTATCCAGAGGACCATTCTGATGATGGGCCGCTATGTGGAACCCATTGAGGATGTGCCCTGTGG
  CAACATTGTGGGGTTGGTCGGGGTGGACCAGTTCCTTGTGAAGACCGGCACCATCACTACCTTTGAGCAC
  GCTCACAACATGCGGGTGATGAAGTTCAGCGTCAGCCCTGTCGTCAGGGTGGCAGTGGAGGCCAAGAACC
  CAGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAAGCGGCTGGCTAAGTCTGACCCTATGGTGCAGTGCAT
  CATCGAGGAGTCTGGGGAGCACATCATTGCTGGTGCTGGAGAGCTGCACCTGGAGATCTGCCTTAAGGAC
  CTGGAGGAGGACCATGCCTGCATCCCCATCAAGAAATCTGACCCTGTCGTGTCCTACCGGGAGACAGTCA
  GTGAGGAGTCCAACGTGCTCTGTCTTTCCAAGTCCCCAAACAAGCACAACAGGCTGTACATGAAGGCCAG
  GCCCTTCCCTGATGGCCTAGCAGAGGACATCGATAAGGGTGAGGTATCTGCCCGCCAGGAGCTCAAGGCG
  CGTGCCCGTTACCTGGCCGAAAAGTATGAGTGGGATGTTGCTGAAGCCCGCAAGATCTGGTGCTTTGGAC
  CTGATGGCACTGGTCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGA
  CAGTGTGGTGGCTGGATTCCAGTGGGCCACTAAGGAGGGTGCTCTTTGTGAGGAGAACATGCGTGGTGTG
  CGATTTGATGTCCATGATGTGACCCTGCATGCTGATGCCATCCACCGAGGAGGTGGTCAGATCATCCCCA
  CAGCGCGCCGCTGCCTCTATGCCAGTGTGCTGACTGCACAGCCCCGCCTCATGGAGCCTATCTACCTGGT
  GGAGATTCAGTGTCCTGAGCAAGTGGTGGGCGGAATCTATGGTGTCCTGAACAGGAAGCGTGGCCATGTC
  TTTGAGGAGTCCCAGGTAGCTGGGACCCCCATGTTCGTGGTCAAGGCCTACCTGCCTGTCAATGAATCCT
  TTGGCTTCACTGCCGACCTTCGATCCAACACTGGTGGCCAGGCCTTCCCCCAGTGTGTGTTTGACCACTG
  GCAGATCCTGCCTGGGGACCCCTTTGACAACAGCAGCCGCCCCAGCCAAGTTGTAGCTGAGACCCGCAAG
  CGCAAAGGCCTGAAGGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202878 representing NM_017245
  Red=Cloning site Green=Tags(s)
  
  MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITI
  KSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTE
  TVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLG
  TVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGAAERAKKVEDMMKKLWGDRYFDPANGKFSKSANSP
  DGKKLPRTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDAL
  LQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGV
  VSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEH
  AHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKD
  LEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKA
  RARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGV
  RFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHV
  FEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRK
  RKGLKEGIPALDNFLDKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017245
• ORF Size: 2574 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017245.1, NM_017245.2, NP_058941.1
• RefSeq Size: 2944
• RefSeq ORF: 2577
• Locus ID: 29565
• MW: 95.3 kDa
• Gene Summary: catalyzes the translocation of peptidyl-tRNA from the ribosomal A site to the P site in translational elongation [RGD, Feb 2006]