Acap2 (NM_001034006) Rat Tagged ORF Clone

CAT#: RR202961

  • TrueORF®

Acap2 (Myc-DDK-tagged ORF) - Rat ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 (Acap2), (10 ug)


  "NM_001034006" in other vectors (3)

Reconstitution Protocol

USD 1,040.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Acap2
Synonyms Centb2; cnt-b2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR202961 representing NM_001034006
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATGACGGTGGATTTCGAGGAGTGTCTGAAGGACTCGCCCCGTTTCAGGGCTGCTTTGGAAGAAG
TAGAAGGAGATGTGGCTGAGCTGGAACTGAAACTGGACAAGCTGGTAAAACTTTGTATCGCGATGATTGA
TACCGGCAAAGCCTTTTGTGCCGCAAATAAACAGTTCATGAATGGGATCCGAGACCTGGCCCAGTATTCT
AGCAATGACGCTGTGGTTGAGACGAGTTTGACCAAGTTTTCTGACAGTCTTCAGGAAATGATCAATTTCC
ACACAATTCTGTTTGACCAAACTCAGAGATCAATTAAGGCCCAGCTTCAGAACTTTGTCAAAGAAGATCT
TAGAAAATTCAAAGATGCCAAGAAACAATTTGAAAAAGTCAGTGAAGAAAAGGAAAACGCTCTAGTAAAA
AACGCCCAAGTTCAAAGAAACAAGCAGCACGAGGTTGAAGAGGCTGCAAACATTCTCACGGCCACGCGGA
AGTGCTTCCGGCACATAGCGCTCGACTATGTCCTGCAGATTAACGTGCTTCAATCAAAAAGGAGATCGGA
AATCCTGAAATCAATGCTGTCCTTCATGTATGCACATCTGGCCTTCTTCCATCAAGGGTATGACCTGTTC
AGTGAGCTTGGGCCCTACATGAAAGATCTTGGCGCACAGTTGGATCGACTGGTTGTGGATGCAGCAAAGG
AGAAAAGAGAAATGGAGCAAAAACATTCTACGATCCAGCAGAAGGATTTCTCCAGTGATGATTCCAAGTT
AGAGTATAACGTAGACGCTGCAAACGGCATTGTCATGGAAGGGTATCTGTTCAAACGAGCCAGCAACGCC
TTCAAAACGTGGAACAGACGCTGGTTCTCCATACAGAACAATCAGCTGGTTTACCAGAAAAAGTTTAAGG
ACAGCCCCACTGTGGTGGTGGAAGACCTCAGGCTCTGCACAGTGAAGCACTGTGAAGACATCGAGCGACG
GTTCTGCTTTGAGGTCGTCTCTCCCACAAAAAGTTGCATGCTCCAAGCAGATTCCGAAAAGCTTCGCCAG
GCCTGGATTAAGGCTGTTCAGACCAGTATTGCAACTGCTTACAGAGAGAAAGGCGATGAATCAGAGAAAC
TGGATAAGAAATCGTCTCCATCGACAGGAAGCCTAGATTCTGGAAGTGAGTCTAAAGAGAAATTACTGAA
AGGAGAAAGTGCTCTGCAGCGTGTGCAGTGTATCCCTGGCAACACCAGCTGCTGCGACTGTGGTCTCGCA
GACCCCCGCTGGGCCAGCATCAACCTGGGCATTACCTTGTGTATTGAGTGCTCTGGGATTCATCGGAGTC
TCGGGGTTCATTTTTCGAAAGTGAGATCTTTAACTTTAGACACTTGGGAGCCTGAGCTCTTAAAGCTTAT
GTGTGAACTGGGGAACGATGTTATAAATCGTGTTTATGAAGCTAAACTGGAAAAAATGGGAGTAAAGAAA
CCACAGCCAGGACAAAGACAGGAGAAGGAGGCATATATCAGAGCAAAATATGTGGAGAGGAAATTCGTGG
ATAAATACTCTACATTGCTGTCACCTTCCGAGCAGGAGAAAAGGATCATCTCCAAGAGCTGTGAGGACCA
GAGACTGAGCCACACCAGAGTGTCTGTCCACACCCCAGTCAAAAGTAATGACAGTGGGATCCAGCAATGC
TCTGATGATGGACGGGAGTCTTTACCTTCCACCGTGTCAGCCAATAGCTTATACGAGCCCGAAGGAGAAA
GGCAAGAATCTTCTGTGTTTCTCGACTCTAAACATCTTAATCCAGGACTTCAGCTTTATAGGGCTTCGTA
TGAAAAAAACCTTCCCAAAATGGCCGAGGCTTTGGCTCATGGTGCAGATGTGAACTGGGCTAATTCAGAT
GAAAACCAAGCAACACCACTCATTCAGGCTGTATTAGGGGGCTCTTTGGTGACATGTGAGTTCCTGTTGC
AGAATGGTGCTAATGTGAACCAAAGAGATGTCCAAGGGCGGGGACCGCTGCACCACGCCACCGTCTTAGG
ACACACAGGGCAGGTATGTTTATTCCTAAAGCGAGGTGCCAATCAACATGCCACTGATGAAGAGGGGAAG
GACCCTCTGAGTATAGCTGTGGAAGCAGCCAATGCTGACATAGTAACGCTGTTACGCTTAGCAAGAATGA
ATGAGGAAATGCGGGAATCCGAAGGACTTTATGGACAGCCAGGTGATGAAACTTACCAGGATATCTTCCG
TGATTTTTCTCAAATGGCATCCAATAATCCAGAGAAACTCAATCGTTTCCAGCAAGATTCACAGAAGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR202961 representing NM_001034006
Red=Cloning site Green=Tags(s)

MKMTVDFEECLKDSPRFRAALEEVEGDVAELELKLDKLVKLCIAMIDTGKAFCAANKQFMNGIRDLAQYS
SNDAVVETSLTKFSDSLQEMINFHTILFDQTQRSIKAQLQNFVKEDLRKFKDAKKQFEKVSEEKENALVK
NAQVQRNKQHEVEEAANILTATRKCFRHIALDYVLQINVLQSKRRSEILKSMLSFMYAHLAFFHQGYDLF
SELGPYMKDLGAQLDRLVVDAAKEKREMEQKHSTIQQKDFSSDDSKLEYNVDAANGIVMEGYLFKRASNA
FKTWNRRWFSIQNNQLVYQKKFKDSPTVVVEDLRLCTVKHCEDIERRFCFEVVSPTKSCMLQADSEKLRQ
AWIKAVQTSIATAYREKGDESEKLDKKSSPSTGSLDSGSESKEKLLKGESALQRVQCIPGNTSCCDCGLA
DPRWASINLGITLCIECSGIHRSLGVHFSKVRSLTLDTWEPELLKLMCELGNDVINRVYEAKLEKMGVKK
PQPGQRQEKEAYIRAKYVERKFVDKYSTLLSPSEQEKRIISKSCEDQRLSHTRVSVHTPVKSNDSGIQQC
SDDGRESLPSTVSANSLYEPEGERQESSVFLDSKHLNPGLQLYRASYEKNLPKMAEALAHGADVNWANSD
ENQATPLIQAVLGGSLVTCEFLLQNGANVNQRDVQGRGPLHHATVLGHTGQVCLFLKRGANQHATDEEGK
DPLSIAVEAANADIVTLLRLARMNEEMRESEGLYGQPGDETYQDIFRDFSQMASNNPEKLNRFQQDSQKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001034006
ORF Size 2310 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001034006.1, NM_001034006.2, NP_001029178.1
RefSeq Size 3579 bp
RefSeq ORF 2313 bp
Locus ID 619382
Cytogenetics 11q22
MW 87.2 kDa

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