Cacna1s (NM_053873) Rat Tagged ORF Clone

CAT#: RR202973

Cacna1s (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1s
• Synonyms: Cchl1a3; RGD1565743
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202973 representing NM_053873
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGTCCTCACCCCAGGATGAGGGCCTGAGGAAGAAGCAGCCTAAGAAACCCGTCCCTGAGATTC
  TGCCGAGGCCACCCCGGGCCTTGTTCTGCTTGACTCTCCAGAACCCCCTGAGGAAGGCCTGCATCAGCGT
  CGTGGAGTGGAAACCCTTCGAGACCATCATCTTGCTCACCATCTTCGCCAACTGTGTGGCCCTGGCAGTG
  TACCTGCCCATGCCAGAGGATGACAACAACACTCTGAACCTTGGCCTGGAGAAACTGGAGTACTTCTTCC
  TCATCGTCTTCTCCATTGAGGCTGCTATGAAGATCATAGCCTATGGCTTCCTATTCCATCAGGATGCTTA
  CCTGCGCAGTGGCTGGAACGTGCTGGACTTCATCATTGTCTTTCTGGGTGTCTTCACCGCGATTCTGGAA
  CAAGTTAACATCATTCAAACCAACACGGCCCCCATGAGCAGCAAAGGAGCCGGCTTGGATGTGAAGGCCC
  TGCGAGCTTTCCGCGTGCTCAGACCCCTGCGGCTGGTGTCAGGGGTGCCCAGCCTTCAGGTAGTCCTCAA
  CTCCATCTTCAAGGCCATGCTCCCCCTGTTCCACATCGCCCTGCTCGTCCTCTTCATGGTCATCATCTAT
  GCCATCATCGGGCTGGAGCTCTTCAAGGGCAAGATGCACAAGACGTGCTACTTCATTGGGACAGACATCG
  TGGCCACAGTAGAGAATGAGAAACCATCACCCTGTGCTAGAACTGGCTCCGGGCGCCCCTGCACCATCAA
  CGGCAGCGAGTGCCGAGGAGGTTGGCCAGGACCCAACCATGGCATCACCCACTTCGACAACTTTGGCTTC
  TCTATGCTCACTGTGTACCAGTGCATCTCCATGGAGGGGTGGACCGATGTCCTGTACTGGGTCAATGATG
  CCATCGGGAACGAGTGGCCCTGGATCTACTTTGTCACCCTCATTCTGCTGGGCTCCTTCTTCATCCTGAA
  CCTGGTGCTGGGTGTCCTGAGTGGAGAATTCACCAAGGAGCGAGAGAAGGCCAAGTCCAGAGGAACCTTC
  CAAAAGCTCCGGGAGAAGCAGCAGCTGGAGGAAGACCTTCGTGGTTACATGAGTTGGATCACACAGGGCG
  AGGTCATGGACGTGGACGACTTGAGAGAAGGCAAGCTGTCTTTGGATGAAGGGGGCTCGGATACAGAGAG
  CTTATATGAAATCGAGGGCTTGAACAAAATCATCCAATTCATTCGGCACTGGCGGCAGTGGAATCGCGTC
  TTCCGCTGGAAGTGCCATGACCTAGTGAAATCCAAGGTCTTCTATTGGCTGGTGATCCTGATTGTGGCCC
  TCAACACCCTGTCCATTGCCTCAGAGCACCACAACCAGCCACTCTGGCTGACGCATTTGCAAGATGTCGC
  CAACCGCGTGCTGCTGGCCCTCTTCACCATCGAGATGCTCATGAAGATGTACGGGCTGGGCCTGCGCCAG
  TACTTCATGTCCATCTTCAACCGCTTCGACTGCTTCGTGGTGTGCAGCGGCATCCTGGAGATCTTGCTGG
  TGGAGTCGGGAGCCATGACGCCCCTGGGCATCTCTGTGCTCCGCTGCATCCGCCTCCTGAGGCTCTTCAA
  GATCACCAAGTACTGGACATCGCTGAGCAACCTGGTGGCCTCGCTGCTCAACTCCATCCGCTCTATCGCT
  TCCCTCTTGCTGCTGCTCTTTCTTTTCATGATCATCTTTGCCCTGCTAGGCATGCAGCTCTTTGGAGGCA
  GATACGACTTTGAAGACACGGAAGTACGACGAAGCAACTTTGACAACTTCCCCCAGGCCCTCATCAGTGT
  CTTCCAGGTCCTGACGGGTGAAGACTGGAACTCTGTGATGTACAATGGGATCATGGCCTACGGAGGCCCG
  TCCTACCCTGGCGTGCTCGTGTGCATTTATTTCATCATCCTTTTTGTCTGTGGTAACTACATCCTTCTCA
  ATGTCTTCCTGGCTATCGCTGTGGACAACCTGGCAGAGGCTGAGAGCCTGACATCTGCCCAGAAGGCCAA
  GGCTGAGGAGAGAAAGCGCAGGAAGATGTCTAGAGGGCTCCCAGACAAGTCAGAAGAGGAGAGATCCACA
  ATGACCAAGAAGCTGGAGCAGAAACCCAAGGGTGAAGGCATCCCCACCACTGCCAAGCTGAAAATCGATG
  AGTTTGAATCGAACGTCAATGAGGTGAAAGACCCCTACCCTTCAGCTGACTTCCCAGGGGACGACGAGGA
  AGATGAGCCTGAGATCCCAGCAAGCCCCCGACCACGCCCTCTGGCTGAGCTGCAGCTGAAAGAGAAAGCG
  GTGCCCATCCCAGAGGCCAGCTCCTTCTTCATCTTCAGCCCCACCAATAAGATCCGCGTCCTATGTCACC
  GCATTGTCAACGCCACCTGGTTCACCAACTTTATCCTACTCTTCATCCTGCTCAGTAGTGCTGCTCTGGC
  CGCTGAGGACCCCATCCGGGCCGACTCCATGAGGAATCAGATCCTTGAGTATTTTGATTATGTGTTCACT
  GCTGTTTTCACTGTGGAGATCGTCCTTAAGATGACCACCTATGGAGCCTTCCTGCACAAAGGCTCCTTCT
  GCCGCAACTACTTCAACATCCTGGACCTGCTGGTAGTGGCTGTGTCTCTCATCTCCATGGGCCTCGAGTC
  CAGCGCCATCTCCGTGGTGAAGATCTTGAGGGTCCTGAGGGTGTTGCGACCACTCCGGGCCATAAACAGA
  GCCAAAGGGTTGAAGCACGTGGTGCAGTGCGTGTTTGTTGCTATCCGCACCATCGGGAACATCGTGCTGG
  TCACCACGCTCCTGCAGTTCATGTTTGCATGCATCGGAGTCCAGCTTTTCAAGGGAAAATTCTACAGCTG
  CAATGATTTATCCAAGATGACTGAAGAGGAGTGCAGGGGCTACTACTACATATACAAGGATGGAGACCCC
  ACGCAGATAGAGTTGCGACCCCGTCAGTGGATACACAATGACTTCCACTTTGACAATGTCCTGTCTGCTA
  TGATGTCACTCTTCACGGTCTCCACCTTTGAGGGTTGGCCTCAGCTACTGTACAAGGCCATAGACTCCAA
  TGAGGAGGACACAGGCCCCGTCTACAACAACCGAGTGGAGATGGCCATCTTCTTCATCATCTACATCATT
  CTCATCGCCTTCTTCATGATGAACATCTTTGTGGGCTTCGTCATCGTCACCTTCCAGGAGCAGGGGGAGA
  CTGAGTATAAGAACTGTGAGCTGGACAAGAACCAGCGCCAATGTGTGCAGTATGCCCTGAAGGCCCGCCC
  GCTGAGGTGTTACATCCCCAAAAACCCATACCAGTATCAGGTGTGGTATGTCGTCACTTCCTCCTACTTT
  GAATACCTGATGTTTGCTCTCATCATGCTCAACACTATCTGCCTAGGCATGCAGCATTACAACCAGTCGG
  AACAGATGAACCACATATCGGACATCCTCAATGTGGCTTTCACCATCATCTTCACCCTGGAGATGATCCT
  CAAGCTCATAGCTTTCAAGCCCAGGGGCTATTTTGGAGACCCCTGGAACGTGTTTGACTTTCTAATCGTC
  ATTGGCAGCATCATTGACGTTATCCTGAGTGAGATCGATACTTTGCTGGCCTCCAGCGGGGGACTATATT
  GCCTGGGTGGAGGCTGCGGGAACGTTGACCCAGATGAGAGCGCCCGCATCTCCAGTGCCTTCTTCCGCCT
  GTTCCGAGTCATGCGACTGATCAAGCTGCTGAGCCGGGCGGAGGGTGTGCGCACCCTGCTCTGGACGTTC
  ATCAAGTCCTTCCAGGCCCTGCCGTATGTGGCTTTGCTGATCGTCATGCTCTTCTTCATCTACGCTGTCA
  TCGGCATGCAGATGTTCGGGAAGATCGCCATGGTGGACGGGACGCAAATAAACCGGAACAACAACTTCCA
  GACTTTCCCACAAGCTGTGCTGCTGCTCTTCAGGTGTGCCACAGGGGAAGCCTGGCAGGAGATCCTGCTG
  GCCTGCAGCTACGGGAAACGCTGCGACCCGGAGTCTGACTACGCACCGGGGGAGGAGTACGCGTGTGGCA
  CCAACTTTGCCTACTACTACTTCATCAGCTTCTACATGCTCTGCGCCTTCCTGATCATCAACCTCTTTGT
  GGCTGTCATCATGGACAACTTTGACTACCTCACAAGGGACTGGTCCATTCTGGGCCCTCACCACCTGGAT
  GAGTTCAAGGCCATCTGGGCTGAGTACGACCCAGAGGCTAAGGGCAGGATCAAGCACCTGGACGTGGTGA
  CCCTGCTGAGGAGGATCCAGCCCCCTCTGGGCTTTGGGAAGTTCTGCCCACACAGGGTAGCATGTAAGAG
  GCTGGTGGGCATGAACATGCCCCTGAATAGTGATGGCACAGTCACCTTCAATGCCACACTCTTCGCCCTG
  GTACGCACAGCCCTCAAGATCAAGACAGAAGGCAATTTTGAACAAGCCAATGAGGAGCTGAGAGCCATCA
  TCAAAAAGATCTGGAAGAGAACCAGCATGAAGCTCCTGGACCAAGTCATCCCTCCAATAGGAGATGACGA
  GGTGACAGTGGGGAAGTTCTATGCCACATTCCTCATCCAGGAGCACTTTCGGAAGTTCATGAAGCGCCAG
  GAGGAATATTATGGGTATCGACCCAAAAAGGACACAGTTCAGATACAGGCTGGATTGCGGACCATAGAGG
  AAGAGGCAGCTCCTGAGATCCACCGAGCCATCTCTGGAGACCTGACGGCGGAGGAGGAGCTGGAGAGGGC
  CATGGTAGAGGCTGCCATGGAGGAGGGGATATTCCGGAGAACTGGAGGCCTGTTTGGTCAAGTGGACAAC
  TTCCTGGAAAGGACCAACTCTCTGCCCCCTGTCATGGCTAACCAAAGACCCCTCCAATTTGCCGAAATGG
  AGATGGAAGAGCTGGAGTCACCAGTCTTTTTGGAGGACTTCCCTCAGAACCCAGGCACCCACCCTCTTGC
  TCGTGCCAATACCAACAACGCCAATGCCAATGTTGCCTATGGCAACAGCAGCCATAGAAACAGCCCAGTG
  TTTTCCAGCATCCGCTATGAAAGGGAGCTCCTGGAAGAGGCAGGCAGGCCTGTTACCAGAGAAGGACCCT
  TCAGTCAGCCCTGCAGCGTCTCTGGAGTTAACAGCAGATCCCATGTGGACAAACTGGAAAGACAGATGTC
  CCAAAGAAGAATGCCCAAAGGCCAGGTGCCTCCTTCCCCATGCCAGCTCTCCCAGGAAAAGCATCCTGTG
  CACGAAGAGGGGAAGGGCCCCAGGTCATGGTCTACGGAAACATCTGACAGTGAGAGCTTTGAGGAGCGTG
  TTCCCAGGAATTCGGCTCACAAGTGCACAGCCCCAGCTACCACCATGCTGATCCAGGAGGCTCTGGTTCG
  AGGAGGACTAGACTCCTTAGCAGCAGATGCTAACTTCGTCATGGCAACAGGCCAGGCCCTGGCAGATGCC
  TGCCAGATGGAACCAGAGGAAGTCGAGGTGGCAGCAACAGAGCTACTGAAGCGAGAATCCCCAAAGGGCG
  GGGCCATGCCCAGGGAGCCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202973 representing NM_053873
  Red=Cloning site Green=Tags(s)
  
  MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAV
  YLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILE
  QVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIY
  AIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGF
  SMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTF
  QKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRV
  FRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLRQ
  YFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIA
  SLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGP
  SYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERST
  MTKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPASPRPRPLAELQLKEKA
  VPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFT
  AVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINR
  AKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDP
  TQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYII
  LIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYF
  EYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLIAFKPRGYFGDPWNVFDFLIV
  IGSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTF
  IKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILL
  ACSYGKRCDPESDYAPGEEYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLD
  EFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFAL
  VRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQ
  EEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDN
  FLERTNSLPPVMANQRPLQFAEMEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNSPV
  FSSIRYERELLEEAGRPVTREGPFSQPCSVSGVNSRSHVDKLERQMSQRRMPKGQVPPSPCQLSQEKHPV
  HEEGKGPRSWSTETSDSESFEERVPRNSAHKCTAPATTMLIQEALVRGGLDSLAADANFVMATGQALADA
  CQMEPEEVEVAATELLKRESPKGGAMPREP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053873
• ORF Size: 5550 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053873.1, NP_446325.1
• RefSeq Size: 6125
• RefSeq ORF: 5553
• Locus ID: 682930
• MW: 210.4 kDa
• Gene Summary: skeletal isoform and subunit of voltage dependent L-type Ca+2 channel; may mediate renal Ca+2 reabsorption; may play a role in bone metabolism [RGD, Feb 2006]