Gpat3 (NM_001025670) Rat Tagged ORF Clone

CAT#: RR203038

Agpat9 (Myc-DDK-tagged ORF) - Rat 1-acylglycerol-3-phosphate O-acyltransferase 9 (Agpat9), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpat3
• Synonyms: Agpat9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203038 representing NM_001025670
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGACAGACCTGGTGGTGAAGCTCCTCTCCACCTGGCTGACGCTGGTGGGCAGCCTCATCCTTT
  TGCCCTCCGCCTTCGGACTATCCCTGGGCATCTCGGAGATCTACATGAAGATCCTGGTGAAAACCTTGGA
  GTGGGCCACGTTACGGATCGAGAAAGGAGCCCCTAAGGAGTCAGTTCTCAAAAGCCCAGCTTCTATGGGT
  ATTATCCAAAGAGATGAGTCACCCATGGAAAAAGGGCTCTCCGGTCTTCGAGGAAGGGACTTCGAGCTCT
  CCGATGTGTTTTATTTCTCCAAGAAGGGGTTGGAAGCCATTGTGGAGGATGAAGTGACCCAGAGGTTCTC
  CTCCGAGGAGCTAGTATCATGGAATCTCCTCACAAGAACCAATGTCAACTTTCACTACATCAGTCCAAAG
  CTCACCATCGTGTGGGTGCTGGGCGTCCTCGTACGCTATTGCTTCCTGCTCCCTCTGAGGGTGACCCTGG
  CTTTCATTGGGATCAGCTTGCTGATTATAGGAACAACACTGGTTGGCCAGCTTCCGGACAGCAGCCTCAA
  GAACTGGCTGAGTGAACTGGTGCACCTGACCTGCTGCAGGATCTGTGTTCGCTCCCTGTCTGGCACCATC
  CATTACCATAACAAGCAGTACAGACCCCAGAAGGGAGGCATCTGTGTCGCCAATCACACCTCTCCCATCG
  ATGTCTTAATCTTGGCGACGGATGGATGTTATGCCATGGTTGGACAGGTTCATGGTGGACTGATGGGGAT
  CATTCAGAGAGCCATGGTTAAGGCTTGTCCACATGTCTGGTTTGAGCGCTCAGAAATAAAGGACCGGCAT
  CTGGTGACTAAGAGGTTAAAAGAACACATCGCTGATAAGAAAAAGTTACCCATTCTAATCTTCCCTGAAG
  GTACCTGCATCAACAATACTTCAGTCATGATGTTCAAAAAGGGAAGCTTTGAAATTGGAGGAACCATATA
  TCCAGTGGCAATAAAGTATAACCCTCAGTTCGGCGATGCCTTCTGGAACAGTAGTAAATACAACCTGGTG
  AGCTATTTGCTTCGGATAATGACAAGCTGGGCCATTGTCTGCGATGTGTGGTACATGCCTCCCATGACCA
  GAGAGGAAGGAGAAGATGCGGTTCAGTTTGCCAACAGGGTTAAATCCGCTATTGCTGTGCAAGGAGGACT
  GACTGAACTTCCCTGGGACGGGGGCCTGAAGAGAGCAAAGGTGAAGGACACCTTTAAGGAAGAACAACAG
  AAGACTTACAGCAAAATGATCGTGGGCAACGGATCTCCCAGCCTGGCCCTGGACTCAAGCACTGTGGACA
  ATCATGGCTCCCCAGAGCCGGCCTTCAGAAGTGAAAGCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203038 representing NM_001025670
  Red=Cloning site Green=Tags(s)
  
  MEGTDLVVKLLSTWLTLVGSLILLPSAFGLSLGISEIYMKILVKTLEWATLRIEKGAPKESVLKSPASMG
  IIQRDESPMEKGLSGLRGRDFELSDVFYFSKKGLEAIVEDEVTQRFSSEELVSWNLLTRTNVNFHYISPK
  LTIVWVLGVLVRYCFLLPLRVTLAFIGISLLIIGTTLVGQLPDSSLKNWLSELVHLTCCRICVRSLSGTI
  HYHNKQYRPQKGGICVANHTSPIDVLILATDGCYAMVGQVHGGLMGIIQRAMVKACPHVWFERSEIKDRH
  LVTKRLKEHIADKKKLPILIFPEGTCINNTSVMMFKKGSFEIGGTIYPVAIKYNPQFGDAFWNSSKYNLV
  SYLLRIMTSWAIVCDVWYMPPMTREEGEDAVQFANRVKSAIAVQGGLTELPWDGGLKRAKVKDTFKEEQQ
  KTYSKMIVGNGSPSLALDSSTVDNHGSPEPAFRSESL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025670
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025670.1, NP_001020841.1
• RefSeq Size: 2017 bp
• RefSeq ORF: 1374 bp
• Locus ID: 305166
• Cytogenetics: 14p22
• MW: 51 kDa