Prkx (NM_001033963) Rat Tagged ORF Clone

CAT#: RR203103

Prkx (Myc-DDK-tagged ORF) - Rat protein kinase, X-linked (Prkx), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203103 representing NM_001033963
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCACCCGCTTGTGCCGAGGCGGCCGTAAAAGATCCTGCAGCCAGAGACCATGACCCCGTGGACG
  CAAAGATCTCAGCGCCCGCCGCGGATCCCCTACCCAGGACGTCGTCACAGAAAGAGGGGCACTCGTTACA
  AGATTGGGACACCATAGCCACCGTGGGCACTGGAACCTTTGGCCGAGTAAACCTGGTGAAGGAGAAGACA
  GGCAGGCGATACTGTGCCTTGAAGATTATGAGCATCCCAGATGTCATCCGCCTGAAGCAGGAACAACATG
  TGCAGAATGAGAAAGCAGTCCTGAAGGAAATAAACCACCCCTTCCTCATTAAACTGCTTTGGACAGACCA
  TGACAACCGCTTCCTGTACATGCTGATGGAGTTTGTGCCTGGTGGTGAGCTGTTCACCTACCTGCGTAAT
  CGTGGCCGCTTTTCCTCAGTTGCTGCAATCTTCTATGCCACAGAGATTGTGTGTGCCATAGAATACCTGC
  ACTCCAAGGAAATCGTATACAGGGACCTGAAGCCTGAAAACATCCTTCTGGACAGGGAAGGACATATCAA
  ACTCACTGACTTCGGCTTCGCCAAGAAACTGGTGGATAGGACATGGACTCTCTGTGGGACACCGGAATAC
  CTTGCCCCAGAAGTTATCCAGAGCAAAGGTCATGGAAGAGCTGTGGACTGGTGGGCATTGGGCATCCTTA
  TATTCGAGATGCTGTCTGGGTTTCCCCCATTTTTCGATGACAACCCATTTGGGATTTACCAGAAAATCCT
  TGCATGCAAAATAGATTTCCCCAGACAATTGGATTTCACCTCTAAGGACCTCATCAAGAAGCTTCTGGTG
  GTAGACAGGACCCGGCGCCTGGGCAACATGAAGAATGGGGCAGAAGACATCAAGCGGCACCGGTGGTTCC
  GAGGTGTGGAGTGGGAGTCCGTGCCACAGAGGAAACTTAAGCCACCCATTGTGCCCAAGTTATCCAGTGA
  TGGTGACATCTCCAACTTTGAGACTTATCCAGAGGGTGAGTTGGACAAGACTCCTTCTGTATCTGATGAA
  GATCTGGAAACATTCAAAAATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203103 representing NM_001033963
  Red=Cloning site Green=Tags(s)
  
  MEPPACAEAAVKDPAARDHDPVDAKISAPAADPLPRTSSQKEGHSLQDWDTIATVGTGTFGRVNLVKEKT
  GRRYCALKIMSIPDVIRLKQEQHVQNEKAVLKEINHPFLIKLLWTDHDNRFLYMLMEFVPGGELFTYLRN
  RGRFSSVAAIFYATEIVCAIEYLHSKEIVYRDLKPENILLDREGHIKLTDFGFAKKLVDRTWTLCGTPEY
  LAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILACKIDFPRQLDFTSKDLIKKLLV
  VDRTRRLGNMKNGAEDIKRHRWFRGVEWESVPQRKLKPPIVPKLSSDGDISNFETYPEGELDKTPSVSDE
  DLETFKNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033963
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033963.1, NP_001029135.1
• RefSeq Size: 4078 bp
• RefSeq ORF: 1077 bp
• Locus ID: 501563
• Cytogenetics: Xq21
• MW: 40.9 kDa