Farsa (NM_001024237) Rat Tagged ORF Clone

CAT#: RR203109

Farsa (Myc-DDK-tagged ORF) - Rat phenylalanyl-tRNA synthetase, alpha subunit (Farsa), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Farsa
• Synonyms: Farsla
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203109 representing NM_001024237
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATAATCCCGTCTTGGAGCAGTTGCTGCGGAGGCTGGAGGTAGCTGATGGCGGCCTGGACAGCG
  CGGAGTTGGCTACGCAGCTGGGCGTGGAGCACCAGGCAGTGGTGGGCGCTGTGAAGAGCCTCCAGGCTCT
  GGGTGAAGTCATTGAAGCCGAGCTTCGTTCTACCAAGTGCTGGGAGCTGACTACTGAGGGTGAGGAGATT
  GCCCGGGAGGGCAGCCATGAGGCCCGTGTGTTTAGAAGCATCCCTCTCGAGGGCCTGGTGCAAAGTGAGC
  TCATGCAACTGCCCAGCGGCAAGGTGGGCTTCAGCAAGGCCATGTCCAACAAGTGGATCCGAGTGGACAA
  GAGTGCAGCCGATGGGCCCCGGGTGTTCCGCGTGGTGGACAGTATAGAGGATGAAGTACAGAGGCGGCTG
  CAGCAGGTCCAGGCAGGCCAGGCTGAGAAGCTGGCTGAAAAGGAAAGGAATGAGCTCCGGAAGAGGAAGC
  TGCTGACCGAAGTGATCCTGAAAACCTACTGGGTGAGCAAGGGCAAGGGCTTCAGCACAAGTGTGTCTAA
  GCAGGAGGCCGAGCTGAGCCCAGAGATGATCTCCAGTGGCTCCTGGAGGGACCGACCCTTCAAACCCTAC
  AACTTCTCTGCCCGTGGTGTGCTCCCAGACAGTGGCCACCTGCACCCCTTACTCAAGGTCCGCTCCCAGT
  TCCGACAAATCTTCTTGGAGATGGGGTTCACAGAGATGCCTACGGACAATTTCATCGAGAGCTCCTTCTG
  GAACTTTGATGCACTCTTCCAGCCTCAGCAGCACCCAGCGCGTGACCAACATGATACTTTCTTCCTGCGA
  GATCCAGCCGAGGCCCTGCAGCTCCCAATGGACTATGTCCAGCGGGTGAAACGGACCCACTCCCAGGGTG
  GCTACGGCTCACAAGGCTACAAGTATACCTGGAAGCTGGAAGAGGCCCGGAAAAACCTGCTTCGCACACA
  TACCACAGCAGCCAGCGCCCGAGCCCTCTACCGTTTAGCCCAGAAGAAGCCATTTACACCAGCCAAGTAC
  TTCTCTATTGATCGTGTGTTTCGGAATGAGACGCTGGACGCCACACACCTGGCCGAGTTCCACCAGATTG
  AGGGTGTGATAGCTGACCATGGTCTCACCCTGGGCCACCTCATGGGTGTACTGAGGGAGTTCTTCACCAA
  GCTGGGAATCACACAGCTGCGCTTCAAGCCGGCCTACAACCCCTACACAGAGCCCAGCATGGAAGTGTTC
  AGCTACCACCAAGGTCTAAAGAAGTGGGTTGAAGTTGGCAACTCTGGAGTCTTCCGCCCTGAGATGCTCC
  TGCCTATGGGGCTCCCTGAGAATGTGTCTGTCATTGCTTGGGGTCTCTCTCTGGAGCGCCCAACAATGAT
  CAAATATGGTATCAATAACATCCGAGAGCTGGTGGGCCACAAGGTGAACCTGCAGATGGTATACGACAGC
  CCCGTGTGCCGACTGGACATTGAGCCCAGGTCCTCAAAGACACAGGAGGCTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203109 representing NM_001024237
  Red=Cloning site Green=Tags(s)
  
  MADNPVLEQLLRRLEVADGGLDSAELATQLGVEHQAVVGAVKSLQALGEVIEAELRSTKCWELTTEGEEI
  AREGSHEARVFRSIPLEGLVQSELMQLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSIEDEVQRRL
  QQVQAGQAEKLAEKERNELRKRKLLTEVILKTYWVSKGKGFSTSVSKQEAELSPEMISSGSWRDRPFKPY
  NFSARGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLR
  DPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYTWKLEEARKNLLRTHTTAASARALYRLAQKKPFTPAKY
  FSIDRVFRNETLDATHLAEFHQIEGVIADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVF
  SYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDS
  PVCRLDIEPRSSKTQEAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024237
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024237.1, NP_001019408.1
• RefSeq Size: 1825 bp
• RefSeq ORF: 1527 bp
• Locus ID: 288917
• Cytogenetics: 19q11
• MW: 57.7 kDa