Slc1a3 (NM_019225) Rat Tagged ORF Clone

CAT#: RR203125

Slc1a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc1a3
• Synonyms: EAAT1; GLAST; GLAST-1; GluT-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203125 representing NM_019225
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAAAAGCAACGGAGAAGAGCCCAGGATGGGAAGCAGGATGGAAAGATTCCAGCAAGGGGTGCGCA
  AGCGGACGCTCCTGGCCAAGAAGAAAGTTCAGAACATCACCAAGGAGGATGTGAAGAGCTACCTGTTTCG
  GAATGCCTTTGTGCTACTCACCGTCAGCGCTGTCATTGTGGGTACAATCCTTGGATTTGCCCTCCGACCG
  TATAAAATGAGCTACCGGGAGGTCAAGTACTTCTCCTTTCCTGGGGAGCTTCTGATGCGGATGCTGCAGA
  TGTTGGTCTTACCCCTGATCATCTCCAGTCTTGTCACAGGAATGGCGGCCCTAGATAGTAAGGCATCTGG
  GAAGATGGGGATGCGAGCTGTGGTCTATTACATGACTACCACCATCATTGCTGTGGTGATCGGCATAATC
  ATTGTCATCATCATCCACCCCGGAAAGGGCACGAAAGAAAACATGTACAGAGAAGGTAAAATCGTGCAGG
  TGACTGCCGCAGATGCCTTCCTGGATTTAATCAGGAACATGTTCCCACCCAATCTGGTAGAAGCCTGCTT
  TAAACAGTTTAAAACCAGCTATGAGAAGAGAAGCTTTAAAGTGCCCATCCAGGCCAACGAAACACTGTTG
  GGCGCCGTGATCAACAACGTGTCAGAGGCCATGGAGACTCTGACCCGGATCCGGGAGGAGATGGTACCCG
  TTCCTGGGTCTGTGAATGGGGTCAATGCCCTGGGCCTCGTTGTCTTCTCCATGTGCTTCGGCTTCGTGAT
  CGGAAACATGAAGGAGCAGGGGCAAGCGCTAAGAGAGTTCTTCGACTCTCTCAATGAAGCCATCATGAGA
  TTGGTAGCGGTGATAATGTGGTATGCACCTCTGGGCATCCTCTTCTTGATCGCAGGGAAGATTGTTGAGA
  TGGAAGATATGGGTGTGATTGGGGGGCAGCTTGCCATGTATACAGTGACAGTCATCGTCGGCCTCCTCAT
  TCATGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCTGGGTTTTCATTGGA
  GGGTTGCTGCAAGCACTCATCACAGCCCTGGGGACCTCCTCAAGTTCTGCCACCCTGCCCATCACTTTCA
  AGTGCCTGGAAGAAAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTTCCCGTGGGGGCCACCAT
  TAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCCGCCATTTTCATCGCTCAAGTTAACAACTTTGAC
  CTGAATTTTGGACAGATTATAACAATAAGTATCACAGCCACAGCCGCAAGCATTGGGGCAGCTGGCATTC
  CTCAGGCCGGTCTAGTCACCATGGTCATCGTGCTGACATCTGTGGGCCTGCCCACGGATGACATCACACT
  CATCATTGCAGTGGACTGGTTTCTGGACCGCCTCCGAACCACCACCAACGTGCTGGGTGACTCCCTCGGG
  GCCGGGATTGTTGAACACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAATGGGGAACTCCGTGA
  TTGAGGAGAATGAAATGAAAAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCAGAGAAACCCGTGGC
  AGACAGCGAAACCAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203125 representing NM_019225
  Red=Cloning site Green=Tags(s)
  
  MTKSNGEEPRMGSRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVSAVIVGTILGFALRP
  YKMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGII
  IVIIIHPGKGTKENMYREGKIVQVTAADAFLDLIRNMFPPNLVEACFKQFKTSYEKRSFKVPIQANETLL
  GAVINNVSEAMETLTRIREEMVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMR
  LVAVIMWYAPLGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIG
  GLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGATINMDGTALYEALAAIFIAQVNNFD
  LNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLG
  AGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNEPEKPVADSETKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019225
• ORF Size: 1629 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019225.1, NM_019225.2, NP_062098.1
• RefSeq Size: 3864
• RefSeq ORF: 1632
• Locus ID: 29483
• MW: 60.1 kDa
• Gene Summary: carries out Na+ dependent transport of glutamate and aspartate; may regulate neurotransmitter concentration in the central nervous system [RGD, Feb 2006]