Rmnd5b (NM_001017473) Rat Tagged ORF Clone

CAT#: RR203154

Rmnd5b (Myc-DDK-tagged ORF) - Rat required for meiotic nuclear division 5 homolog B (S. cerevisiae) (Rmnd5b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rmnd5b
• Synonyms: MGC112682
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203154 representing NM_001017473
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGTGCGCATGTGTGGAGAGGGAGCTCGACAAGGTCCTGCACAAGTTCCTGACCTATGGGCAAC
  ATTGTGAGCAGAGCCTGGAGGAGCTGCTGCACTACGTGGGCCAGTTGAGAGCTGAACTGGCCAGTGCAGC
  TCTCCAGGGGACCCCTCTCTCAGCCACGCTGTCCCTAGTAATGTCCCAGTGCTGTCGGAAGATCAAAGAC
  ACAGTCCAGAAGCTGGCTTCCGACCATAAGGACATTCACAGCAGCGTCTCCCGAGTGGGCAAAGCCATTG
  ACCGGAACTTTGACTCTGAGATCTGCGGCGTGGTCTCAGATGCTGTGTGGGACTCCCGTGAGAAGCAGCA
  GCAGATCCTGCAGATGGCCATTGTCGAGCACCTGTACCAGCAGGGCATGCTCAGCGTAGCTGAGGAGCTG
  TGCCAGGAATCAACATTGAATGTGGACCTGGACTTCAAGCAGCCCTTCTTGGAGTTGAATCGGATCTTGG
  AGGCTCTGCATGAGCAAGACCTAGGACCTGCACTGGAATGGGCCGTTTCCCACAGGCAGCGCCTGCTGGA
  GCTCAACAGCTCACTGGAGTTCAAGCTGCACCGGCTGCACTTTATCCGCCTCCTAGCTGGTGGCCCGGAG
  AAGCAGCTGGAGGCCCTCAGCTATGCCCGACACTTCCAGCCTTTTGCTCGGCTACACCAGAGAGAGATCC
  AGGTGATGATGGGCAGTCTGGTGTACCTGCGGCTGGGCTTGGAGAAGTCACCCTACTGCCACCTCTTAGA
  CAACAGCCACTGGGCAGAGATCTGTGAGACCTTTACTCGGGATGCATGCTCCCTCCTGGGGCTTTCAGTG
  GAGTCCCCACTCAGTGTCAGCTTTGCTTCTGGCTGTGTGGCACTGCCAGTGCTGATGAACATTAAAGCTG
  TGATCGAACAGAGGCAGTGCACTGGAGTGTGGAGTCACAAGGATGAGTTGCCGATTGAGATTGAGCTGGG
  CATGAAGTGCTGGTATCACTCTGTGTTTGCGTGCCCTATCCTGCGACAGCAGACCTCAGATTCCAACCCC
  CCTATCAAGCTCATCTGTGGCCACGTCATCTCCAGAGATGCACTCAACAAGCTCATCAATGGAGGAAAGC
  TAAAGTGTCCCTATTGTCCCATGGAGCAGAACCCAGCAGATGGGAAACGTATCATATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203154 representing NM_001017473
  Red=Cloning site Green=Tags(s)
  
  MEQCACVERELDKVLHKFLTYGQHCEQSLEELLHYVGQLRAELASAALQGTPLSATLSLVMSQCCRKIKD
  TVQKLASDHKDIHSSVSRVGKAIDRNFDSEICGVVSDAVWDSREKQQQILQMAIVEHLYQQGMLSVAEEL
  CQESTLNVDLDFKQPFLELNRILEALHEQDLGPALEWAVSHRQRLLELNSSLEFKLHRLHFIRLLAGGPE
  KQLEALSYARHFQPFARLHQREIQVMMGSLVYLRLGLEKSPYCHLLDNSHWAEICETFTRDACSLLGLSV
  ESPLSVSFASGCVALPVLMNIKAVIEQRQCTGVWSHKDELPIEIELGMKCWYHSVFACPILRQQTSDSNP
  PIKLICGHVISRDALNKLINGGKLKCPYCPMEQNPADGKRIIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017473
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017473.1, NP_001017473.1
• RefSeq Size: 1806 bp
• RefSeq ORF: 1182 bp
• Locus ID: 497900
• Cytogenetics: 10q21
• MW: 44.5 kDa