Polr2b (NM_001106002) Rat Tagged ORF Clone

CAT#: RR203199

Polr2b (Myc-DDK-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (Polr2b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polr2b
• Synonyms: RpB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203199 representing NM_001106002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGACGCCGACGAGGATATGCAATATGATGAAGATGATGATGAAATCACTCCAGATTTGTGGCAGG
  AAGCATGCTGGATCGTGATCAGTTCTTATTTTGATGAAAAAGGCTTGGTCAGACAGCAGCTGGATTCCTT
  TGACGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGATGCACCACCGATTGACCTGCAGGCTGAA
  GCCCAGCACGCTAGTGGGGAGGTTGAAGAGCCGCCAAGATATTTACTGAAGTTTGAACAAATTTACCTCT
  CCAAACCTACTCATTGGGAAAGAGATGGTGCCCCTTCACCAATGATGCCAAATGAAGCCAGGTTAAGAAA
  TCTCACGTACTCCGCCCCGCTTTACGTGGACATAACGAAGACGGTCATTAAAGAGGGTGAAGAGCAGCTG
  CAGACTCAGCACCAGAAAACCTTCATCGGGAAAATCCCCATTATGCTGCGCTCCACGTATTGCTTGTTGA
  ATGGCTTAACAGACCGTGACTTGTGTGAGCTGAATGAGTGTCCTTTGGATCCTGGTGGCTATTTTATTAT
  TAATGGATCAGAAAAGGTTCTGATAGCTCAAGAGAAAATGGCAACAAACACAGTCTATGTGTTTGCCAAA
  AAGGATTCCAAGTATGCCTATACAGGAGAATGCCGCTCATGCCTAGAGAATTCTTCCCGACCCACCAGTA
  CTATATGGGTTAGCATGCTGGCCAGAGGAGGACAGGGTGCAAAGAAGAGTGCAATTGGCCAGCGCATCGT
  GGCGACATTACCATATATCAAGCAAGAAGTTCCCATCATAATTGTGTTCAGAGCATTAGGCTTTGTGTCT
  GATAGAGATATTTTAGAGCACATCATTTATGACTTCGAAGACCCAGAGATGATGGAAATGGTTAAACCAT
  CTCTCGATGAAGCATTTGTCATCCAAGAGCAGAATGTTGCCTTAAACTTCATTGGTTCAAGAGGGGCGAA
  GCCTGGTGTTACTAAGGAGAAAAGAATTAAATATGCAAAAGAAGTCTTACAGAAAGAGATGCTTCCGCAT
  GTCGGTGTCAGTGATTTCTGTGAGACCAAAAAGGCCTATTTCTTGGGGTACATGGTTCATAGGTTACTTC
  TAGCAGCCTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGCAACAAGAGATTGGATCTTGCTGG
  GCCTCTGCTTGCGTTCTTATTTAGAGGCATGTTTAAGAATTTGCTGAAAGAAGTACGGATCTATGCACAG
  AAGTTTATTGACCGCGGAAAGGACTTTAACCTAGAGTTGGCAATTAAGACAAGGATCATATCCGATGGGC
  TAAAATACTCTTTAGCCACTGGGAACTGGGGTGACCAGAAGAAAGCTCACCAAGCCAGAGCTGGAGTGTC
  GCAGGTACTGAACCGCCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAACTCTCCTATTGGC
  AGAGATGGCAAGCTAGCAAAGCCGAGACAGTTGCATAATACATTGTGGGGGATGGTGTGTCCTGCTGAAA
  CTCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCCTGATGGCTTATATTTCAGTTGGATCTCA
  ACCTTCTCCAATTTTGGAATTTTTGGAAGAATGGAGCATGGAAAACTTAGAGGAAATTTCCCCTGCAGCT
  ATTGCCGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATCCATAAAGACCCGGAACAACTCA
  TGAACACACTCAGGAAATTACGGCGTCAGATGGACATCATTGTATCCGAAGTTTCTATGATCAGAGATAT
  CCGCGAGCGTGAGATTCGGATCTATACAGACGCAGGCCGTATTTGTAGACCACTTCTAATTGTGGAAAAG
  CAAAAGCTTCTTTTGAAGAAGAGACACATTGACCAGTTGAAAGAGAGAGAATATAACAACTACAGTTGGC
  AGGATCTGGTAGCCAGTGGGGTAGTAGAGTATATTGATACCCTGGAAGAAGAAACCGTGATGCTTGCAAT
  GACTCCAGATGATTTGCAGGAGAAAGAAGTAGCTTATTGCTCCACCTATACCCACTGTGAGATTCACCCA
  TCAATGATTCTTGGCGTCTGTGCATCTATTATTCCTTTTCCTGATCATAACCAGTCCCCTAGAAACACGT
  ACCAGTCTGCTATGGGCAAGCAGGCTATGGGAGTTTATATCACCAACTTCCACGTTCGGATGGACACCTT
  GGCGCACGTTCTCTACTATCCTCAGAAGCCCCTTGTGACTACACGGTCTATGGAGTATCTCCGGTTTAGA
  GAACTGCCAGCAGGAATCAACTCAATTGTGGCCATTGCATCATACACGGGCTATAATCAAGAAGACTCTG
  TTATCATGAATCGTTCAGCTGTGGATCGAGGCTTTTTCAGGTCTGTTTTCTACCGATCATACAAAGAACA
  AGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTGTTCGAGAAGCCTACACGTGAAACATGTCAGGGCATG
  AGGCATGCCATTTATGACAAGTTGGACGATGATGGCTTGATAGCGCCAGGGGTCCGAGTGTCGGGAGACG
  ATGTTATTATAGGCAAAACGGTCACCCTCCCTGAGAACGAGGATGAGCTGGAGAGCACCAACAGGCGCTA
  CACCAAGAGGGACTGCAGCACCTTCCTCAGAACCAGTGAGACAGGCATTGTGGACCAGGTCATGGTCACT
  CTCAACCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGAATTCCGCAGATCGGAGACA
  AATTTGCTAGCCGACATGGTCAGAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGACATGCCGTTCAC
  TTGTGAAGGCATAACTCCTGACATCATCATAAACCCCCACGCCATCCCCTCTCGGATGACGATTGGTCAC
  TTGATTGAATGTCTCCAAGGGAAGGTCTCTGCGAACAAGGGTGAAATTGGTGATGCTACTCCGTTTAATG
  ATGCTGTCAATGTGCAGAAGATTTCTAATCTTTTATCTGACTACGGCTACCATCTCAGAGGAAATGAGGT
  GCTCTACAATGGATTCACTGGTCGCAAAATCACATCGCAGATTTTTATTGGCCCCACTTACTACCAGCGT
  TTGAAGCATATGGTGGATGACAAAATTCATTCTCGTGCTCGGGGGCCCATTCAGATTCTTAACAGACAGC
  CCATGGAGGGCAGATCGCGTGATGGTGGCCTGCGCTTTGGAGAAATGGAGCGAGACTGTCAGATCGCACA
  TGGTGCAGCACAGTTCTTAAGAGAAAGGCTGTTTGAAGCATCAGATCCGTATCAGGTTCACGTTTGTAAT
  CTTTGTGGAATAATGGCAATTGCTAACACCAGGACTCACACATACGAATGCAGGGGATGCCGCAATAAGA
  CCCAGATCTCCTTGGTGCGAATGCCGTATGCGTGCAAGCTGTTGTTCCAGGAGCTCATGTCCATGAGCAT
  CGCACCACGGATGATGAGTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203199 representing NM_001106002
  Red=Cloning site Green=Tags(s)
  
  MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAE
  AQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQL
  QTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAK
  KDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVS
  DRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPH
  VGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ
  KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIG
  RDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAA
  IADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEK
  QKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHP
  SMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFR
  ELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM
  RHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVT
  LNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGH
  LIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQR
  LKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCN
  LCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106002
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001106002.1, NP_001099472.1
• RefSeq Size: 3829
• RefSeq ORF: 3525
• Locus ID: 289561
• MW: 133.9 kDa