Ablim2 (NM_001001514) Rat Tagged ORF Clone

CAT#: RR203206

Ablim2 (Myc-DDK-tagged ORF) - Rat actin binding LIM protein family, member 2 (Ablim2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ablim2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203206 representing NM_001001514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCAGTGTCGCAGCCCCAGGCTGCTCATGCCCCACTGGAGAAGCCGGCCAGCACTGCGATCCTGT
  GTAATACCTGTGGGAATGTGTGCAAGGGAGAGGTGCTGCGGGTGCAGAACAAGCACTTCCACATTAGGTG
  CTTTGTGTGCAAAGCATGCGGCTGTGACCTGGCTGAGGGTGGCTTCTTCGTGAGGCAGGGTGAGCACATT
  TGCACACGTGACTACCAGCGGCTCTATGGGACTCGCTGCTTCAGCTGTGACTGCTTCATTGAAGGGGAAG
  TGGTGTCAGCGCTTGGGAAGACCTACCACCCTGACTGCTTCGTATGCGCTGTCTGCAGGCTGCCTTTTCC
  TCCTGGGGACCGTGTGACCTTCAATGGGAAGGATTGTATGTGCCAGAAGTGTTCTCCACCCACATTGGTG
  GGCAACAGTGCCCATGTGGCCCAGGGCCTCCGGAGTTGTGGGGGCTGTGGCTTAGAAATCAAGAATGGCC
  AGGCACTGGTGGCTTTGGACAAGCACTGGCACCTGGGATGCTTCAAGTGCAAGACTTGTGGGAAACTCCT
  CAATGCAGAGTACATCAGCAAGGATGGGCTGCCCTACTGTGAGGCTGATTATCACACCAAGTTTGGCATC
  CGCTGCGATGGCTGTGAGAAGTACATCACTGGTCGGGTGCTGGAGGCTGGAGAGAAGCACTACCACCCTT
  CATGTGCGCTATGCGTCAGGTGCGGCCAGATGTTCTCAGAGGGCGAGGAGATGTACCTGCAAGGTTCCTC
  CATCTGGCATCCGGCATGTCGGCAAGCAGCCAGGACTGAAGACAAGAGCAAGGAAACCAGGACTTCCTCA
  GAGAGCATTGTCTCTGTGCCTGCCTCCAGCACATCGGGGTCCCCAAGCCGTGTGATCTATGCAAAGCTTG
  GTGATGAGATCCTGGACTACAGGGATTTGGCTGCTCTTCCCAAAAACAAAGCGATCTACAACATCGACCG
  CCCCGACATGATCTCCTATTCGCCCTACATTAGCCACTCGGCTGTGGGGGACAGGCAGAGCTATGGTGAG
  GGGGATCAGGATGATCGGTCCTACAAGCAGTGCAGGACCTCCAGCCCCAGCTCTGCTGGCTCAGTCAGCC
  TTGGGCACTACACTCCAACGTCACGATCACCCCAGCACTACAGTCGTCCAGGCAGCGAAAGTGGCCGTAG
  CACTCCAAGTCTCTCAGTGCACTCCGACAGCAGGCCTCCTTCCTCTACCTATCAGCAGGCTCCGAGACAC
  TTCCACGTCCCAGACACTGGCGTAAAAGATAACATCTATAGGAAACCCCCTATCTACAAACAGCACGCCG
  CCAGGCGACTGGACGTGGAGGACAGCAGTTTCGACCAGGATAGCAGGAAGAAGACAACGTGGCTGCTCCT
  CAAGGGAGATGCAGACACCAGAACTAACTCTCCGGACTTGGACAGTCAGTCTCTGTCCCTCAGCAGTGGG
  GCTGATCAAGAACCTCTCCAAAGGATGCCAGGGGACAGCCTCTACTCACAGTATAAGATTTATCCCTATG
  ATTCCCTCATCGTAACAAACCGAATTCGTGTGAAACTGCCCAAAGATGTAGACCGGACACGGCTGGAGAG
  ACACCTATCACCTGAAGAGTTCCAAGAAGTGTTTGGGATGAGCATCGAGGAGTTTGACCGCCTGGCCCTG
  TGGAAGAGGAATGACCTCAAGAAGAAAGCCTTGCTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203206 representing NM_001001514
  Red=Cloning site Green=Tags(s)
  
  MSAVSQPQAAHAPLEKPASTAILCNTCGNVCKGEVLRVQNKHFHIRCFVCKACGCDLAEGGFFVRQGEHI
  CTRDYQRLYGTRCFSCDCFIEGEVVSALGKTYHPDCFVCAVCRLPFPPGDRVTFNGKDCMCQKCSPPTLV
  GNSAHVAQGLRSCGGCGLEIKNGQALVALDKHWHLGCFKCKTCGKLLNAEYISKDGLPYCEADYHTKFGI
  RCDGCEKYITGRVLEAGEKHYHPSCALCVRCGQMFSEGEEMYLQGSSIWHPACRQAARTEDKSKETRTSS
  ESIVSVPASSTSGSPSRVIYAKLGDEILDYRDLAALPKNKAIYNIDRPDMISYSPYISHSAVGDRQSYGE
  GDQDDRSYKQCRTSSPSSAGSVSLGHYTPTSRSPQHYSRPGSESGRSTPSLSVHSDSRPPSSTYQQAPRH
  FHVPDTGVKDNIYRKPPIYKQHAARRLDVEDSSFDQDSRKKTTWLLLKGDADTRTNSPDLDSQSLSLSSG
  ADQEPLQRMPGDSLYSQYKIYPYDSLIVTNRIRVKLPKDVDRTRLERHLSPEEFQEVFGMSIEEFDRLAL
  WKRNDLKKKALLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001514
• ORF Size: 1719 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001514.1, NM_001001514.2, NP_001001514.1
• RefSeq Size: 2568 bp
• RefSeq ORF: 1722 bp
• Locus ID: 360958
• Cytogenetics: 14q21
• MW: 63.8 kDa
• Gene Summary: contains a zinc binding domain [RGD, Feb 2006]