Asns (NM_013079) Rat Tagged ORF Clone

CAT#: RR203498

Asns (Myc-DDK-tagged ORF) - Rat asparagine synthetase (Asns), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asns
• Synonyms: MGC93148
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203498 representing NM_013079
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGGCATCTGGGCCCTCTTCGGCAGCGATGACTGCCTTTCCGTGCAGTGTCTGAGTGCGATGAAGA
  TTGCGCACAGGGGCCCAGATGCATTCCGTTTTGAGAACGTCAATGGATACACCAACTGCTGTTTTGGCTT
  CCACCGGCTGGCGGTGGTTGACCCCCTGTTTGGAATGCAGCCAATAAGAGTGAGGAAATATCCTTATCTG
  TGGCTGTGTTACAACGGTGAAATCTACAACCACAAGGCGCTACAACAACGTTTCGAATTTGAGTATCAGA
  CCAATGTGGACGGTGAGATAATTCTCCATCTCTATGACAAAGGCGGCATCGAGAAAACCATCTGTATGTT
  GGATGGGGTGTTTGCATTTATCTTACTGGACACTGCCAATAAGAAAGTATTCCTGGGCAGAGATACCTAT
  GGTGTCAGGCCTTTGTTTAAAGCCTTGACAGAAGATGGATTTCTGGCTGTGTGTTCAGAAGCCAAAGGCC
  TTGTCTCCTTGAAACACTCCACCACCCCCTTCCTAAAAGTGGAGCCCTTTCTTCCTGGACACTATGAAGT
  TTTGGATTTAAAACCAAATGGCAAAGTCGCGTCTGTGGAAATGGTCAAATACCATCACTGTACGGATGAA
  CCACTGCATGCCATCTATGACAGTGTGGAGAAACTCTTCCCAGGCTTTGAGATAGAGACCGTGAAAAACA
  ATCTGCGTATCCTTTTTAACAACGCTATCAAGAAACGCTTGATGACTGACCGGAGGATTGGCTGCCTTTT
  ATCAGGAGGCCTGGACTCCAGCTTGGTTGCTGCCTCCCTGCTGAAGCAACTCAAGGAGGCCCAAGTGCCC
  TATGCTCTCCAGACATTTGCTATCGGCATGGAAGACAGCCCTGATCTACTGGCTGCCAGAAAGGTGGCAA
  ATTATATTGGAAGTGAGCATCATGAAGTCCTTTTTAACTCTGAAGAAGGCATTCAGTCCCTGGACGAAGT
  CATATTTTCCTTGGAAACTTATGATATTACGACAGTTCGAGCATCTGTAGGTATGTATTTAATTTCCAAG
  TATATTCGGAAGAACACAGACAGCGTGGTGATCTTCTCCGGAGAGGGGTCAGATGAGCTTACACAGGGCT
  ATATATATTTCCACAAGGCGCCTTCTCCTGAGAAGGCGGAGGAGGAGAGTGAGAGGCTCCTGAAGGAACT
  CTACCTGTTTGATGTCCTCCGTGCCGACCGCACTACTGCTGCTCACGGTCTCGAACTGAGAGTCCCGTTT
  CTGGATCATCGGTTTTCTTCCTATTACCTGTCTCTGCCACCAGAAATGAGAATTCCAAAAGATGGCATAG
  AAAAACATCTCCTGAGAGAGACTTTTGAGGACTCCAACCTGCTACCCAAAGAGATTCTCTGGCGACCCAA
  GGAAGCCTTCAGTGATGGGATCACCTCAGTCAAGAACTCCTGGTTCAAGATTTTACAGGACTTCGTTGAA
  CATCAGGTTGATGATGCGATGATGTCTGAGGCCTCCCAGAAATTTCCCTTCAATACTCCCCAAACTAAAG
  AAGGCTATTACTACCGTCAGATCTTTGAACACCATTACCCCGGCCGGGCTGATTGGCTGACCCATTATTG
  GATGCCCAAGTGGATCAATGCCACCGACCCTTCTGCCCGCACTCTGACCCATTACAAGTCAACTGCCAAA
  GCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203498 representing NM_013079
  Red=Cloning site Green=Tags(s)
  
  MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVDPLFGMQPIRVRKYPYL
  WLCYNGEIYNHKALQQRFEFEYQTNVDGEIILHLYDKGGIEKTICMLDGVFAFILLDTANKKVFLGRDTY
  GVRPLFKALTEDGFLAVCSEAKGLVSLKHSTTPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDE
  PLHAIYDSVEKLFPGFEIETVKNNLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKEAQVP
  YALQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFSLETYDITTVRASVGMYLISK
  YIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLKELYLFDVLRADRTTAAHGLELRVPF
  LDHRFSSYYLSLPPEMRIPKDGIEKHLLRETFEDSNLLPKEILWRPKEAFSDGITSVKNSWFKILQDFVE
  HQVDDAMMSEASQKFPFNTPQTKEGYYYRQIFEHHYPGRADWLTHYWMPKWINATDPSARTLTHYKSTAK
  A
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013079
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013079.1, NM_013079.2, NP_037211.2
• RefSeq Size: 2040
• RefSeq ORF: 1686
• Locus ID: 25612
• MW: 64.2 kDa
• Gene Summary: crucial for asparagine synthesis; may be important for progression through the G1 phase of the cell cycle [RGD, Feb 2006]