Set (NM_001012504) Rat Tagged ORF Clone

CAT#: RR203618

Set (Myc-DDK-tagged ORF) - Rat SET nuclear oncogene (Set), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Set
• Synonyms: Ab1-115
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203618 representing NM_001012504
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGAAGCGGCAATCTGCGATCCTGCCTCAGCCCAAGAAACCCAGACCCGTTGCTGCCCCGAAGC
  TGGAGGACAAGTCGGCCTCTCCCGGCCTGCCGAAGGGAGAAAAAGAACAGCAAGAAGCAATTGAACATAT
  TGATGAAGTACAAAATGAAATAGACAGGCTTAATGAACAAGCCAGTGAGGAAATTTTGAAAGTAGAACAA
  AAATATAACAAACTCCGCCAACCATTTTTTCAGAAGAGGTCAGAATTGATCACCAAAATCCCAAATTTTT
  GGGTAACAACATTTGTCAACCATCCACAAGTGTCTGCACTGCTTGGGGAGGAAGACGAGGAGGCCCTGCA
  CTATTTGACCAGAGTTGAAGTGACAGAATTTGAAGACATTAAATCAGGTTACAGAATAGATTTTTATTTT
  GATGAAAATCCTTACTTTGAAAATAAAGTTCTCTCCAAAGAATTTCATCTGAATGAGAGCGGTGACCCAT
  CTTCAAAGTCCACCGAAATCAAATGGAAATCTGGAAAGGATTTGACAAAATGCTCAAGTCAAACGCAGAA
  TAAGGCCAGCAGGAAGAGGCAGCACGAAGAGCCAGAGAGCTTCTTTACCTGGTTTACTGACCATTCAGAC
  GCAGGTGCTGACGAGTTAGGAGAGGTCATCAAAGATGATATTTGGCCAAATCCTTTGCAGTACTATTTGG
  TTCCCGATATGGATGATGAAGAAGGAGAGGCAGAGGATGATGATGACGACGATGAAGAGGAGGAAGGGTT
  GGAAGACATTGATGAAGAAGGAGACGAGGACGAAGGTGAAGAAGACAATGATGAGGATGAAGGGGAGGAA
  GGAGAGGAGGATGAAGGCGAGGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203618 representing NM_001012504
  Red=Cloning site Green=Tags(s)
  
  MAPKRQSAILPQPKKPRPVAAPKLEDKSASPGLPKGEKEQQEAIEHIDEVQNEIDRLNEQASEEILKVEQ
  KYNKLRQPFFQKRSELITKIPNFWVTTFVNHPQVSALLGEEDEEALHYLTRVEVTEFEDIKSGYRIDFYF
  DENPYFENKVLSKEFHLNESGDPSSKSTEIKWKSGKDLTKCSSQTQNKASRKRQHEEPESFFTWFTDHSD
  AGADELGEVIKDDIWPNPLQYYLVPDMDDEEGEAEDDDDDDEEEEGLEDIDEEGDEDEGEEDNDEDEGEE
  GEEDEGEDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012504
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012504.1, NP_001012522.1
• RefSeq Size: 1519 bp
• RefSeq ORF: 870 bp
• Locus ID: 307947
• Cytogenetics: 19q12
• MW: 33.4 kDa