Fah (NM_017181) Rat Tagged ORF Clone

CAT#: RR203746

Fah (Myc-DDK-tagged ORF) - Rat fumarylacetoacetate hydrolase (Fah), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fah
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203746 representing NM_017181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTTATTCCGGTGGCCGAGGACTCCGACTTTCCCATCCAAAACCTGCCCTATGGCGTTTTCTCCA
  CTCAAAGCAACCCAAAGCCACGGATTGGTGTGGCCATCGGTGACCAGATCTTGGACCTGAGTGTCATTAA
  ACACCTCTTTACCGGACCTGTCCTCTCCAAACATCAGCATGTCTTCGATGAGACAACTCTCAATAGCTTT
  ATGGGCCTCGGCCAAGCGGCATGGAAGGAGGCAAGAGCATCCTTACAGAACTTACTGTCTGCCAGCCAAG
  CCCAGCTCAGAGATGACAAGGAGCTTCGGCAGCGTGCATTCACCTCCCAGGCTTCTGCCACGATGCACCT
  TCCTGCTACCATAGGAGACTACACGGACTTCTACTCCTCTCTGCAGCATGCCACTAACGTTGGCATTATG
  TTCAGGGGCAAGGAGAATGCGCTGTTGCCCAATTGGCTCCACTTACCTGTGGGATACCATGGCCGAGCTT
  CCTCCGTTGTGGTGTCTGGTACCCCAATTCGAAGACCCATGGGACAGATGAGACCTGATAACTCAAAGCC
  TCCTGTGTATGGTGCCAGCAAACGCTTAGACATGGAGTTGGAAATGGCTTTCTTTGTAGGCCCTGGGAAC
  AGATTCGGCGAGCCAATCCCCATTTCCAAGGCCCAGGAGCACATTTTCGGGATGGTCCTCATGAACGACT
  GGAGTGCTCGAGACATCCAGCAATGGGAGTACGTCCCCCTTGGGCCATTCCTGGGGAAAAGTTTTGGAAC
  CACCATCTCCCCATGGGTGGTGCCCATGGATGCTCTCATGCCCTTTGTGGTGCCAAACCCAAAGCAGGAC
  CCTAAGCCCCTGCCATATCTCTGCCACAGCCAGCCCTACACATTTGATATCAACCTGTCCGTTGCTTTGA
  AAGGAGAAGGAATGAGCCAGGCAGCTACCATCTGCAGGTCCAACTTTAAGCACATGTACTGGACCATTCT
  GCAGCAACTGACACACCACTCTGTTAATGGATGCAATCTGAGACCTGGGGACCTCTTGGCTTCTGGAACC
  ATCAGTGGATCAGACCCTGAAAGCTTTGGCTCCATGCTGGAACTGTCCTGGAAGGGAACAAAGGCTATCG
  ATGTGGGGCAGGGGCAAACCAGGACCTTTCTTCTGGACGGAGATGAAGTCATCATAACAGGTCACTGCCA
  GGGGGATGGCTACCGTGTTGGTTTTGGCCAATGTGCTGGGAAAGTGCTGCCTGCCCTCTCGCCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203746 representing NM_017181
  Red=Cloning site Green=Tags(s)
  
  MSFIPVAEDSDFPIQNLPYGVFSTQSNPKPRIGVAIGDQILDLSVIKHLFTGPVLSKHQHVFDETTLNSF
  MGLGQAAWKEARASLQNLLSASQAQLRDDKELRQRAFTSQASATMHLPATIGDYTDFYSSLQHATNVGIM
  FRGKENALLPNWLHLPVGYHGRASSVVVSGTPIRRPMGQMRPDNSKPPVYGASKRLDMELEMAFFVGPGN
  RFGEPIPISKAQEHIFGMVLMNDWSARDIQQWEYVPLGPFLGKSFGTTISPWVVPMDALMPFVVPNPKQD
  PKPLPYLCHSQPYTFDINLSVALKGEGMSQAATICRSNFKHMYWTILQQLTHHSVNGCNLRPGDLLASGT
  ISGSDPESFGSMLELSWKGTKAIDVGQGQTRTFLLDGDEVIITGHCQGDGYRVGFGQCAGKVLPALSPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017181
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017181.1, NM_017181.2, NP_058877.1
• RefSeq Size: 1493
• RefSeq ORF: 1260
• Locus ID: 29383
• MW: 46 kDa
• Gene Summary: deficiency leads to human hereditary tyrosinemia type 1 [RGD, Feb 2006]