Asah2 (NM_053646) Rat Tagged ORF Clone

CAT#: RR203862

Asah2 (Myc-DDK-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asah2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203862 representing NM_053646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGCGAACCTTCTCCTCCTTGGAGGCGTTCCTCATTTTCCTTCTGGTAATGATGACGGCCATCA
  CAGTGGCCCTTCTCACCCTCTTGTTCGTCACCAGTGGGACCATCGAAAACCACAAAGATTCAGGGAATCA
  CTGGGTTTCAACCACCCAGGGCCCCACAACCACCCAGTCCTCTCCAACCACACAAACTCCAACCACACAA
  ACTCCAGACCTCCCTCCCTCTCAGAACTTCAGTGGCTACTACATTGGTGTTGGGCGAGCTGACTGCACCG
  GACAAGTGTCAGATATCAATTTGATGGGCTATGGCAAAAATGGCCAGAACGCACAGGGTCTCCTCACCAG
  GCTGTTCAGCCGCGCTTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAA
  CTATGCATGATTTCCCAACGACTCAGGTTGGAGGTCTTGAAGAGACTACAGAGTAAATATGGCTCTCTGT
  ATCGAAGAGACAACGTTATCCTGAGTGCCACTCACACTCACTCTGGCCCAGCAGGATTTTTCCAATATAC
  ACTCTATATACTTGCCAGCGAGGGATTCAGCAACCGGACCTTTCAGTACATAGTCTCTGGGATCGTGAAG
  AGCATTGATATAGCACACACAAATCTTAAACCGGGCAAAGTCCTTATCAACAAAGGAAATGTTGCTAATG
  TGCAGATCAACCGCAGTCCCTCCTCTTACCTTCAGAATCCACCTTCGGAGAGAGCAAGGTATTCTTCCGA
  CACGGACAAGGAAATGGTCGTCTTGAAACTGGTGGATTTGAATGGAGAAGACTTGGGCCTTATCAGCTGG
  TTTGCCGTCCACCCCGTGAGCATGAACAACAGTAACCACCTCGTCAACAGTGACAATATGGGCTACGCGG
  CTTACCTTTTCGAGCAAGAAAAGAACAGAGGCTATCTGCCTGGACAGGGACCATTCGTAGCAGGCTTTGC
  TTCATCAAATCTCGGAGACGTGTCGCCCAACATTCTTGGCCCACATTGTGTCAACACAGGGGAGTCTTGT
  GACAACGACAAAAGCACCTGTCCCAGTGGTGGGCCTAGCATGTGCATGGCCAGTGGACCCGGACAAGATA
  TGTTTGAGAGCACACACATTATAGGACGGGTCATCTATCAGAAAGCCAAGGAGCTGCATGCCTCTGCCTC
  CCAGGAAGTGACCGGCCCAGTGCTCACAGCTCACCAGTGGGTGAACATGACGGATGTGAGCGTCCAACTC
  AATGCCACACACACAGTGAAGACGTGTAAAGCCGCCCTGGGCTACAGTTTTGCCGCAGGCACAATTGATG
  GAGTTTCGGGCCTCAATATTACACAGGGAACAACAGAAGGGAATCTATTTTGGGACACTCTTCGGGACCA
  GCTCTTGGGAAAACCATCTGAAGAAATCATAGAATGCCAGAAACCTAAACCAATCCTAATCCACACGGGA
  GAGCTGACAAAACCTCATCCTTGGCAACCAGATATTGTTGATATTCAGATCGTTACTCTTGGTTCGTTGG
  CCATAGCTGCTATCCCTGGGGAATTTACAACCATGTCAGGTCGAAGACTTCGTGAGGCAGTTAAAAAAGA
  ATTTGCACTTTATGGGATGAAGGATATGACTGTCGTTATCGCAGGGCTGAGCAATGTTTATACCCATTAC
  ATTACCACATATGAAGAATACCAGGCTCAGCGGTATGAGGCAGCATCTACGATTTATGGACCACACACCC
  TGTCTGCGTACATCCAGCTCTTCAGAGCCCTTGCTAAGGCCATTGCTACGGATACAGTAGCCAACATGAG
  CAGCGGTCCTGAGCCTCCATTCTTCAAAAACCTGATAGGTTCACTTATTCCTAATATTGCGGATAGAGCA
  CCAATAGGCAAACAATTTGGGGATGTCCTGCAGCCAGCAAAACCTGAATACAGAGTGGGAGAAGTGGTTG
  AAGTTGTCTTTGTAGGCGCTAACCCAAAGAATTCAGCAGAAAACCAGACCCATCAAACCTTCCTCACTGT
  GGAGAAATACGAGGACTCTGTAGCCAACTGGCAGATAATGCATAACGATGCCTCCTGGGAGACGAGATTT
  TATTGGCACAAGGGAGTACTGGGTCTGAGCAATGCAACAATACATTGGCATATTCCAGATACTGCCCTCC
  CTGGAGTCTACAGAATCAGATATTTTGGACACAATCGGAAGCAGGAACTTCTAAAGCCTGCTGTCATACT
  AGCATTTGAAGGAATTTCTTCCCCTTTTGAAATTGTCACTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203862 representing NM_053646
  Red=Cloning site Green=Tags(s)
  
  MAKRTFSSLEAFLIFLLVMMTAITVALLTLLFVTSGTIENHKDSGNHWVSTTQGPTTTQSSPTTQTPTTQ
  TPDLPPSQNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNAQGLLTRLFSRAFILADPDGSNRMAFVSVE
  LCMISQRLRLEVLKRLQSKYGSLYRRDNVILSATHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIVK
  SIDIAHTNLKPGKVLINKGNVANVQINRSPSSYLQNPPSERARYSSDTDKEMVVLKLVDLNGEDLGLISW
  FAVHPVSMNNSNHLVNSDNMGYAAYLFEQEKNRGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESC
  DNDKSTCPSGGPSMCMASGPGQDMFESTHIIGRVIYQKAKELHASASQEVTGPVLTAHQWVNMTDVSVQL
  NATHTVKTCKAALGYSFAAGTIDGVSGLNITQGTTEGNLFWDTLRDQLLGKPSEEIIECQKPKPILIHTG
  ELTKPHPWQPDIVDIQIVTLGSLAIAAIPGEFTTMSGRRLREAVKKEFALYGMKDMTVVIAGLSNVYTHY
  ITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRALAKAIATDTVANMSSGPEPPFFKNLIGSLIPNIADRA
  PIGKQFGDVLQPAKPEYRVGEVVEVVFVGANPKNSAENQTHQTFLTVEKYEDSVANWQIMHNDASWETRF
  YWHKGVLGLSNATIHWHIPDTALPGVYRIRYFGHNRKQELLKPAVILAFEGISSPFEIVTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053646
• ORF Size: 2283 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053646.1, NM_053646.2, NP_446098.1
• RefSeq Size: 4914
• RefSeq ORF: 2286
• Locus ID: 114104
• MW: 83.5 kDa
• Gene Summary: membrane-bound neutral ceramidase; hydrolyzes the N-acyl linkage of ceramide to produce sphingosine [RGD, Feb 2006]