Bag1 (NM_001106647) Rat Tagged ORF Clone

CAT#: RR203877

Bag1 (Myc-DDK-tagged ORF) - Rat BCL2-associated athanogene (Bag1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bag1
• Synonyms: Bag-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203877 representing NM_001106647
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGCGGATCGCGGCGGCGCGCGGCGACCGCGAGGCGACCAGGAGCCGCTGGGACCCCGGCTGCGCGCCC
  CTCGGTCGGCCCGGGAGACGCGCCAGTCGGAGTCCCGGGCCGAGCGTGGCTTGCCTCCCTCTCAGCGTTC
  ATCTGTCCGCAGTGCAGCCAGCGGGCATGACCGATCCACCAGGGGCGCCGCCTCCGGCGCTTGCAAGCCG
  CGGGTGAAGAAGAAAGTCCGGCCCCGCTCTTCTCAGAGCGAGAAGGTAGCTCACAGCAAGGAGTTGACTA
  GAAGTAAGAAGTTGACCCGCAGCAAGAAAGTGACCGGGACCCAGGAGGCGACCCAAGTAGAGGAGGTGAC
  CACGATCGAGGAGGCGACCCAGACCGAGGAGATTACGGTAGCGGAAGAGGTGACCCAGACCGAGAACATG
  GCCCAGACCGAGGAGATGGTCCAGACGGAGGAAATGGAACCACCCACACTCAGCGTGGTCGTCACCCACA
  GCAATGAGAGGTATGACCTTCTTGTTACCCCACAGCAAGGTAACAGTGAGCCAATTGTCCAAGACCTGGC
  TCAGCTTGTTGAAGAGGCCACAGGAGTTCCACTACCTTTTCAGAAGCTCATATTTAAGGGCAAATCTCTG
  AAAGAAATGGAAACACCCTTGTCAGCACTTGGAATGCAAAATGGTTGCCGAGTCATGTTAATTGGTGAAA
  AGAGCAATCCAGAAGAAGAGGCTGAGTTGAAAAAGCTGAAGGACTTGGAGGTATCTGTGGAGAAGACAGC
  TAACCACCTGGAAGAGTTGAATAAAGAGCTTTCTGACATCCAGCAGGGTTTTCTGGCTAAGGAATTACAA
  GCGGAGGCTCTCTGCAGACTTGATAGGAAAATAAAGGCCACAATTGAGCAATTCATGAAGATCTTGGAGG
  AGATTGACACAATGGTCCTACCAGAAAACTTTAAAGACAGCAGGCTAAAAAGGAAGAATTTGGTGAAAAA
  GGTTCAGGTGTTCCTAGCAGAGTGTGATACAGTGGAGCAGTACATCTGCCAAGAGACAGAGCGGCTGCAG
  TCTACAAACTTGGCCCTGCCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203877 representing NM_001106647
  Red=Cloning site Green=Tags(s)
  
  LADRGGARRPRGDQEPLGPRLRAPRSARETRQSESRAERGLPPSQRSSVRSAASGHDRSTRGAASGACKP
  RVKKKVRPRSSQSEKVAHSKELTRSKKLTRSKKVTGTQEATQVEEVTTIEEATQTEEITVAEEVTQTENM
  AQTEEMVQTEEMEPPTLSVVVTHSNERYDLLVTPQQGNSEPIVQDLAQLVEEATGVPLPFQKLIFKGKSL
  KEMETPLSALGMQNGCRVMLIGEKSNPEEEAELKKLKDLEVSVEKTANHLEELNKELSDIQQGFLAKELQ
  AEALCRLDRKIKATIEQFMKILEEIDTMVLPENFKDSRLKRKNLVKKVQVFLAECDTVEQYICQETERLQ
  STNLALPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106647
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001106647.2, NM_001106647.3, NP_001100117.2
• RefSeq Size: 1308
• RefSeq ORF: 1077
• Locus ID: 297994
• MW: 40.1 kDa
• Gene Summary: The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. Studies in human and mouse suggest that the protein encoded by this gene (referred to as Bcl2-associated athanogene) binds to Bcl2 protein. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative, downstream, AUG translation initiation site. [provided by RefSeq, Jul 2008]