Plk2 (NM_031821) Rat Tagged ORF Clone

CAT#: RR203879

Plk2 (Myc-DDK-tagged ORF) - Rat polo-like kinase 2 (Drosophila) (Plk2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plk2
• Synonyms: Snk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203879 representing NM_031821
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCTGCGGACTATCACCTACCAGCCGGCCGCCGGCACCAAGATGTGCGAGCAGGCGCTGGGCA
  AAGCTTGCGGCGGGGACTCAAAGAAGAAGCGACCGCAGCAGCCTTCTGAAGACGGGCAGTCCCAAGCCCA
  GGTGACCCCGGCGGCCCCACACCACCATCATCACCATTCTCACTCCGGACCCGAGATCTCGCGGATTATA
  GTCGACCCCACGACGGGGAAGCGCTACTGCCGGGGCAAAGTGCTGGGCAAGGGTGGATTTGCAAAGTGTT
  ACGAAATGACAGATCTGACAAACAACAAAGTCTACGCTGCAAAAATTATCCCTCACAGCAGAGTAGCTAA
  ACCTCATCAAAGGGAAAAGATCGACAAAGAAATTGAGCTTCACAGAATCCTGCACCATAAGCATGTAGTG
  CAGTTTTACCACTACTTTGAAGACAAAGAAAACATTTACATCCTCTTGGAGTACTGCAGTAGAAGGTCCA
  TGGCTCATATCTTGAAAGCAAGAAAGGTGTTGACAGAGCCAGAAGTCCGATACTACCTCAGGCAGATTGT
  GTCGGGACTCAAGTATCTTCACGAACAAGAAATCTTGCATAGGGATCTCAAGCTAGGGAACTTTTTTATT
  AATGAGGCCATGGAGCTGAAGGTTGGAGACTTCGGCTTGGCTGCCAGACTGGAACCTTTGGAACACAGAA
  GGAGAACAATATGTGGAACCCCAAATTATCTCTCCCCTGAAGTCCTCAACAAACAAGGACATGGCTGTGA
  ATCAGACATCTGGGCCTTAGGCTGTGTAATGTATACAATGCTGCTAGGAAGACCTCCATTCGAAACAACA
  AATCTGAAAGAAACGTACAGGTGCATAAGAGAAGCAAGATATACAATGCCGTCTTCATTACTGGCCCCCG
  CCAAGCACTTGATAGCCAGCATGCTGTCCAAAAACCCAGAGGACCGCCCCAGTTTGGATGACATCATTCG
  GCATGACTTCTTTCTGCAGGGCTTCACTCCAGACAGACTCTCTTCTAGCTGTTGCCACACAGTTCCAGAC
  TTCCACTTGTCAAGCCCAGCTAAGAATTTCTTTAAGAAAGCTGCTGCCGCTCTTTTTGGTGGCAAAAAGG
  ACAAAGCAAGATATAATGACACACACAATAAAGTGTCTAAGGAAGATGAAGACATCTACAAGCTTCGGCA
  TGATTTGAAGAAAACATCGATAACCCAGCAACCCAGCAAACACAGAACAGATGAGGAGCTCCAGCCTCCT
  CCAACTACCTTTGCCAAATCTGGGACATCCGCAGTGGAAAACAAACAGCAGATTGGAGATGCAATCCGGA
  TGATAGTTAGAGGGACGCTGGGCAGCTGCAGCAGCAGCAGTGAATGCCTTGAGGATAGCACCATGGGAAG
  TGTTGCAGACACAGTGGCAAGAGTCCTTCGAGGGTGTCTAGAAAACATGCCTGAAGCTGACTGTATTCCC
  AAAGAGCAGTTGAGCACGTCCTTTCAGTGGGTCACCAAATGGGTCGACTACTCCAACAAATACGGCTTTG
  GATACCAGCTCTCAGACCACACTGTTGGAGTCCTTTTCAACAACGGCGCTCACATGAGCCTCCTTCCAGA
  CAAAAAGACAGTTCACTATTATGCGGAACTTGGCCAATGCTCTGTTTTCCCAGCAACAGATGCCCCCGAA
  CAATTTATTAGTCAAGTGACGGTGCTGAAATACTTTTCCCACTACATGGAGGAGAACCTCATGGATGGTG
  GCGATCTCCCTAGTGTTACTGACATTCGAAGACCTCGGCTCTACCTCCTTCAGTGGTTAAAGTCTGATAA
  AGCCTTAATGATGCTCTTCAATGACGGCACATTTCAGGTGAATTTCTACCACGATCATACAAAGATAATT
  ATCTGTAACCAAAATGAAGAATACCTTCTCACATACATCAACGAAGACAGGATATCTACAACTTTCAGAC
  TGACGACCCTGTTGATGTCTGGCTGTTCGCTAGAATTGAAACATCGAATGGAATACGCCCTGAACATGCT
  CTTGCAGAGATGTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203879 representing NM_031821
  Red=Cloning site Green=Tags(s)
  
  MELLRTITYQPAAGTKMCEQALGKACGGDSKKKRPQQPSEDGQSQAQVTPAAPHHHHHHSHSGPEISRII
  VDPTTGKRYCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHKHVV
  QFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEILHRDLKLGNFFI
  NEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPFETT
  NLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFFLQGFTPDRLSSSCCHTVPD
  FHLSSPAKNFFKKAAAALFGGKKDKARYNDTHNKVSKEDEDIYKLRHDLKKTSITQQPSKHRTDEELQPP
  PTTFAKSGTSAVENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEADCIP
  KEQLSTSFQWVTKWVDYSNKYGFGYQLSDHTVGVLFNNGAHMSLLPDKKTVHYYAELGQCSVFPATDAPE
  QFISQVTVLKYFSHYMEENLMDGGDLPSVTDIRRPRLYLLQWLKSDKALMMLFNDGTFQVNFYHDHTKII
  ICNQNEEYLLTYINEDRISTTFRLTTLLMSGCSLELKHRMEYALNMLLQRCN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031821
• ORF Size: 2046 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031821.1, NP_114009.1
• RefSeq Size: 2781
• RefSeq ORF: 2049
• Locus ID: 83722
• MW: 78.4 kDa
• Gene Summary: binds Ca2+ and integrin-binding protein Cib; may phosphorylate proteins in the dendrites of activated neurons; may play a role in long term synaptic plasticity [RGD, Feb 2006]